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Zinc in PDB 3efo: Crystal Structure of the Mammalian Copii-Coat Protein SEC23/24 Bound to the Transport Signal Sequence of Syntaxin 5

Protein crystallography data

The structure of Crystal Structure of the Mammalian Copii-Coat Protein SEC23/24 Bound to the Transport Signal Sequence of Syntaxin 5, PDB code: 3efo was solved by J.Goldberg, J.D.Mancias, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 50.00 / 2.70
Space group P 21 21 21
Cell size a, b, c (Å), α, β, γ (°) 103.060, 140.830, 152.250, 90.00, 90.00, 90.00
R / Rfree (%) 21.1 / 26.1

Zinc Binding Sites:

The binding sites of Zinc atom in the Crystal Structure of the Mammalian Copii-Coat Protein SEC23/24 Bound to the Transport Signal Sequence of Syntaxin 5 (pdb code 3efo). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Crystal Structure of the Mammalian Copii-Coat Protein SEC23/24 Bound to the Transport Signal Sequence of Syntaxin 5, PDB code: 3efo:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in 3efo

Go back to Zinc Binding Sites List in 3efo
Zinc binding site 1 out of 2 in the Crystal Structure of the Mammalian Copii-Coat Protein SEC23/24 Bound to the Transport Signal Sequence of Syntaxin 5


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Crystal Structure of the Mammalian Copii-Coat Protein SEC23/24 Bound to the Transport Signal Sequence of Syntaxin 5 within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Zn766

b:46.3
occ:1.00
SG A:CYS88 2.3 50.5 1.0
SG A:CYS66 2.3 48.2 1.0
SG A:CYS85 2.3 44.1 1.0
SG A:CYS61 2.4 40.7 1.0
CB A:CYS85 3.1 46.2 1.0
CB A:CYS61 3.2 39.5 1.0
CB A:CYS66 3.2 46.7 1.0
CB A:CYS88 3.5 47.9 1.0
N A:CYS88 3.8 49.9 1.0
CA A:CYS88 4.2 52.5 1.0
CB A:GLN90 4.3 61.5 1.0
CA A:CYS85 4.6 47.8 1.0
N A:ARG67 4.6 51.8 1.0
CB A:PHE87 4.6 44.7 1.0
CA A:CYS66 4.6 48.8 1.0
CB A:ALA68 4.6 42.5 1.0
CA A:CYS61 4.7 43.4 1.0
N A:ALA68 4.7 47.3 1.0
CB A:ARG63 4.7 56.9 1.0
C A:CYS88 4.7 52.5 1.0
C A:PHE87 4.8 47.5 1.0
N A:TYR89 4.9 55.4 1.0
N A:GLN90 4.9 61.3 1.0
C A:CYS66 5.0 52.0 1.0

Zinc binding site 2 out of 2 in 3efo

Go back to Zinc Binding Sites List in 3efo
Zinc binding site 2 out of 2 in the Crystal Structure of the Mammalian Copii-Coat Protein SEC23/24 Bound to the Transport Signal Sequence of Syntaxin 5


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Crystal Structure of the Mammalian Copii-Coat Protein SEC23/24 Bound to the Transport Signal Sequence of Syntaxin 5 within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Zn1034

b:57.4
occ:1.00
SG B:CYS367 2.3 59.1 1.0
SG B:CYS389 2.3 63.6 1.0
SG B:CYS386 2.4 56.8 1.0
SG B:CYS364 2.6 60.8 1.0
CB B:CYS386 3.0 58.7 1.0
CB B:CYS364 3.2 57.8 1.0
CB B:CYS367 3.2 45.8 1.0
CB B:CYS389 3.6 67.9 1.0
N B:CYS367 3.6 51.0 1.0
N B:CYS389 3.7 63.2 1.0
CA B:CYS367 4.0 46.8 1.0
CA B:CYS389 4.2 65.9 1.0
CA B:CYS386 4.5 60.3 1.0
CB B:PHE388 4.5 47.3 1.0
CB B:ARG366 4.6 61.9 1.0
CB B:CYS391 4.6 76.1 1.0
N B:LYS368 4.7 46.3 1.0
CA B:CYS364 4.7 60.5 1.0
C B:PHE388 4.7 57.0 1.0
C B:CYS367 4.7 48.6 1.0
C B:CYS389 4.8 68.1 1.0
N B:PHE388 4.8 51.1 1.0
C B:ARG366 4.8 55.0 1.0
N B:CYS391 4.9 76.1 1.0
N B:ASN390 4.9 70.9 1.0
CA B:PHE388 4.9 53.2 1.0
C B:CYS386 4.9 60.6 1.0
CB B:ALA369 5.0 55.0 1.0

Reference:

J.D.Mancias, J.Goldberg. Structural Basis of Cargo Membrane Protein Discrimination By the Human Copii Coat Machinery. Embo J. V. 27 2918 2008.
ISSN: ISSN 0261-4189
PubMed: 18843296
DOI: 10.1038/EMBOJ.2008.208
Page generated: Thu Oct 24 12:47:02 2024

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