Atomistry » Zinc » PDB 3c3u-3cia » 3c8z
Atomistry »
  Zinc »
    PDB 3c3u-3cia »
      3c8z »

Zinc in PDB 3c8z: The 1.6 A Crystal Structure of Mshc: the Rate Limiting Enzyme in the Mycothiol Biosynthetic Pathway

Enzymatic activity of The 1.6 A Crystal Structure of Mshc: the Rate Limiting Enzyme in the Mycothiol Biosynthetic Pathway

All present enzymatic activity of The 1.6 A Crystal Structure of Mshc: the Rate Limiting Enzyme in the Mycothiol Biosynthetic Pathway:
6.1.1.16;

Protein crystallography data

The structure of The 1.6 A Crystal Structure of Mshc: the Rate Limiting Enzyme in the Mycothiol Biosynthetic Pathway, PDB code: 3c8z was solved by L.W.Tremblay, F.Fan, M.W.Vetting, J.S.Blanchard, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 37.09 / 1.60
Space group H 3
Cell size a, b, c (Å), α, β, γ (°) 123.521, 123.521, 186.026, 90.00, 90.00, 120.00
R / Rfree (%) 18.5 / 20.7

Zinc Binding Sites:

The binding sites of Zinc atom in the The 1.6 A Crystal Structure of Mshc: the Rate Limiting Enzyme in the Mycothiol Biosynthetic Pathway (pdb code 3c8z). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the The 1.6 A Crystal Structure of Mshc: the Rate Limiting Enzyme in the Mycothiol Biosynthetic Pathway, PDB code: 3c8z:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in 3c8z

Go back to Zinc Binding Sites List in 3c8z
Zinc binding site 1 out of 2 in the The 1.6 A Crystal Structure of Mshc: the Rate Limiting Enzyme in the Mycothiol Biosynthetic Pathway


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of The 1.6 A Crystal Structure of Mshc: the Rate Limiting Enzyme in the Mycothiol Biosynthetic Pathway within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Zn413

b:16.4
occ:1.00
NE2 A:HIS256 2.1 14.2 1.0
SG A:CYS43 2.3 15.8 1.0
SG A:CYS231 2.3 15.5 1.0
SG A:5CA418 2.4 15.7 1.0
CD2 A:HIS256 3.1 16.7 1.0
CE1 A:HIS256 3.1 13.4 1.0
CB A:CYS43 3.2 14.7 1.0
CB A:CYS231 3.3 17.3 1.0
CB A:5CA418 3.5 16.9 1.0
O A:HOH639 3.9 43.7 1.0
CA A:CYS43 4.1 15.0 1.0
NE1 A:TRP227 4.2 17.3 1.0
CG A:HIS256 4.2 14.8 1.0
ND1 A:HIS256 4.2 15.0 1.0
ND2 A:ASN81 4.3 17.5 1.0
OD1 A:ASN81 4.4 16.3 1.0
CE2 A:TYR41 4.6 18.5 1.0
CG A:ASN81 4.7 17.2 1.0
O A:HOH438 4.7 20.1 1.0
OH A:TYR41 4.7 17.9 1.0
CA A:CYS231 4.7 17.4 1.0
CE2 A:TRP227 4.8 16.4 1.0
CA A:5CA418 4.9 17.7 1.0
CZ2 A:TRP227 5.0 15.9 1.0
CD1 A:TRP227 5.0 16.8 1.0
C A:CYS43 5.0 15.3 1.0
NE2 A:HIS257 5.0 18.3 1.0

Zinc binding site 2 out of 2 in 3c8z

Go back to Zinc Binding Sites List in 3c8z
Zinc binding site 2 out of 2 in the The 1.6 A Crystal Structure of Mshc: the Rate Limiting Enzyme in the Mycothiol Biosynthetic Pathway


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of The 1.6 A Crystal Structure of Mshc: the Rate Limiting Enzyme in the Mycothiol Biosynthetic Pathway within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Zn413

b:13.3
occ:1.00
NE2 B:HIS256 2.1 11.4 1.0
SG B:CYS43 2.3 13.8 1.0
SG B:CYS231 2.3 13.5 1.0
SG B:5CA416 2.4 13.1 1.0
CD2 B:HIS256 3.1 11.8 1.0
CE1 B:HIS256 3.1 11.5 1.0
CB B:CYS43 3.2 12.6 1.0
CB B:CYS231 3.4 14.9 1.0
CB B:5CA416 3.5 13.0 1.0
O B:HOH743 3.9 41.0 1.0
CA B:CYS43 4.0 12.6 1.0
NE1 B:TRP227 4.2 14.0 1.0
ND1 B:HIS256 4.2 11.6 1.0
CG B:HIS256 4.2 11.1 1.0
ND2 B:ASN81 4.3 13.4 1.0
OD1 B:ASN81 4.4 13.6 1.0
CE2 B:TYR41 4.5 14.0 1.0
OH B:TYR41 4.7 12.0 1.0
O B:HOH471 4.7 17.2 1.0
CG B:ASN81 4.7 12.1 1.0
CA B:CYS231 4.8 13.7 1.0
CE2 B:TRP227 4.9 12.9 1.0
CA B:5CA416 4.9 13.8 1.0
CD1 B:TRP227 5.0 13.5 1.0
C B:CYS43 5.0 12.8 1.0
NE2 B:HIS257 5.0 13.3 1.0

Reference:

L.W.Tremblay, F.Fan, M.W.Vetting, J.S.Blanchard. The 1.6 A Crystal Structure of Mycobacterium Smegmatis Mshc: the Penultimate Enzyme in the Mycothiol Biosynthetic Pathway. Biochemistry V. 47 13326 2008.
ISSN: ISSN 0006-2960
PubMed: 19053270
DOI: 10.1021/BI801708F
Page generated: Thu Oct 24 11:45:40 2024

Last articles

Zn in 9JYW
Zn in 9IR4
Zn in 9IR3
Zn in 9GMX
Zn in 9GMW
Zn in 9JEJ
Zn in 9ERF
Zn in 9ERE
Zn in 9EGV
Zn in 9EGW
© Copyright 2008-2020 by atomistry.com
Home   |    Site Map   |    Copyright   |    Contact us   |    Privacy