Zinc in PDB 3abk: Bovine Heart Cytochrome C Oxidase at the No-Bound Fully Reduced State (50K)

All present enzymatic activity of Bovine Heart Cytochrome C Oxidase at the No-Bound Fully Reduced State (50K):
1.9.3.1;

The structure of Bovine Heart Cytochrome C Oxidase at the No-Bound Fully Reduced State (50K), PDB code: 3abk was solved by K.Ohta, K.Muramoto, K.Shinzawa-Itoh, E.Yamashita, S.Yoshikawa, T.Tsukihara, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	40.00 / 2.00
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	182.247, 207.944, 177.997, 90.00, 90.00, 90.00
R / Rfree (%)	18.3 / 21.9

The structure of Bovine Heart Cytochrome C Oxidase at the No-Bound Fully Reduced State (50K) also contains other interesting chemical elements:

Magnesium	(Mg)	2 atoms
Iron	(Fe)	4 atoms
Copper	(Cu)	6 atoms
Sodium	(Na)	2 atoms

The binding sites of Zinc atom in the Bovine Heart Cytochrome C Oxidase at the No-Bound Fully Reduced State (50K) (pdb code 3abk). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Bovine Heart Cytochrome C Oxidase at the No-Bound Fully Reduced State (50K), PDB code: 3abk:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Bovine Heart Cytochrome C Oxidase at the No-Bound Fully Reduced State (50K)


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Bovine Heart Cytochrome C Oxidase at the No-Bound Fully Reduced State (50K) within 5.0Å range: probe atom residue distance (Å) B Occ F:Zn99 b:37.6 occ:1.00 SG F:CYS60 2.3 33.9 1.0 SG F:CYS82 2.3 35.5 1.0 SG F:CYS62 2.4 37.7 1.0 SG F:CYS85 2.4 35.9 1.0 CB F:CYS82 3.2 33.8 1.0 CB F:CYS62 3.3 37.3 1.0 CB F:CYS60 3.4 35.3 1.0 CB F:CYS85 3.4 35.5 1.0 CA F:CYS62 3.6 39.1 1.0 N F:CYS85 3.7 37.0 1.0 N F:CYS62 4.0 39.3 1.0 CA F:CYS85 4.1 36.8 1.0 O F:CYS60 4.4 37.2 1.0 O F:HOH2514 4.5 49.3 1.0 C F:CYS60 4.5 36.1 1.0 CB F:SER84 4.5 37.6 1.0 CA F:CYS60 4.5 34.8 1.0 CA F:CYS82 4.6 33.3 1.0 OG F:SER84 4.7 39.4 1.0 C F:SER84 4.7 37.6 1.0 CG2 F:THR87 4.8 39.4 1.0 C F:ILE61 4.8 38.1 1.0 C F:CYS85 4.8 37.2 1.0 CB F:ILE70 4.9 34.9 1.0 C F:CYS62 5.0 42.2 1.0

Zinc binding site 2 out of 2 in the Bovine Heart Cytochrome C Oxidase at the No-Bound Fully Reduced State (50K)


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Bovine Heart Cytochrome C Oxidase at the No-Bound Fully Reduced State (50K) within 5.0Å range: probe atom residue distance (Å) B Occ S:Zn99 b:43.4 occ:1.00 SG S:CYS62 2.3 43.3 1.0 SG S:CYS60 2.4 41.8 1.0 SG S:CYS85 2.4 43.9 1.0 SG S:CYS82 2.4 39.8 1.0 CB S:CYS82 3.1 37.8 1.0 CB S:CYS85 3.3 44.0 1.0 CB S:CYS62 3.3 46.3 1.0 CB S:CYS60 3.4 40.5 1.0 CA S:CYS62 3.6 47.7 1.0 N S:CYS85 3.7 44.8 1.0 CA S:CYS85 4.1 43.3 1.0 N S:CYS62 4.1 46.9 1.0 O S:CYS60 4.4 36.4 1.0 C S:CYS60 4.4 39.9 1.0 O S:HOH3514 4.4 46.7 1.0 OG1 S:THR87 4.5 49.2 1.0 CA S:CYS60 4.5 39.6 1.0 CB S:SER84 4.6 43.0 1.0 CA S:CYS82 4.6 38.5 1.0 C S:SER84 4.7 44.0 1.0 CG2 S:THR87 4.8 50.1 1.0 C S:CYS85 4.8 43.6 1.0 C S:ILE61 4.8 45.6 1.0 OG S:SER84 4.9 44.7 1.0 CB S:ILE70 5.0 35.1 1.0 C S:CYS62 5.0 50.5 1.0

K.Ohta, K.Muramoto, K.Shinzawa-Itoh, E.Yamashita, S.Yoshikawa, T.Tsukihara. X-Ray Structure of the No-Bound Cu(B) in Bovine Cytochrome C Oxidase Acta Crystallogr.,Sect.F V. 66 251 2010.
ISSN: ESSN 1744-3091
PubMed: 20208153
DOI: 10.1107/S1744309109055109