Zinc in PDB 2zxw: Bovine Heart Cytochrome C Oxidase at the Fully Oxidized State (1-S X- Ray Exposure Dataset)

All present enzymatic activity of Bovine Heart Cytochrome C Oxidase at the Fully Oxidized State (1-S X- Ray Exposure Dataset):
1.9.3.1;

The structure of Bovine Heart Cytochrome C Oxidase at the Fully Oxidized State (1-S X- Ray Exposure Dataset), PDB code: 2zxw was solved by H.Aoyama, K.Muramoto, K.Shinzawa-Itoh, K.Hirata, E.Yamashita, T.Tsukihara, T.Ogura, S.Yoshikawa, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	40.00 / 2.50
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	184.156, 207.621, 178.247, 90.00, 90.00, 90.00
R / Rfree (%)	18.5 / 23.3

The structure of Bovine Heart Cytochrome C Oxidase at the Fully Oxidized State (1-S X- Ray Exposure Dataset) also contains other interesting chemical elements:

Magnesium	(Mg)	2 atoms
Iron	(Fe)	4 atoms
Copper	(Cu)	6 atoms
Sodium	(Na)	2 atoms

The binding sites of Zinc atom in the Bovine Heart Cytochrome C Oxidase at the Fully Oxidized State (1-S X- Ray Exposure Dataset) (pdb code 2zxw). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Bovine Heart Cytochrome C Oxidase at the Fully Oxidized State (1-S X- Ray Exposure Dataset), PDB code: 2zxw:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Bovine Heart Cytochrome C Oxidase at the Fully Oxidized State (1-S X- Ray Exposure Dataset)


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Bovine Heart Cytochrome C Oxidase at the Fully Oxidized State (1-S X- Ray Exposure Dataset) within 5.0Å range: probe atom residue distance (Å) B Occ F:Zn99 b:26.2 occ:1.00 SG F:CYS82 2.3 24.8 1.0 SG F:CYS60 2.3 20.9 1.0 SG F:CYS62 2.3 26.3 1.0 SG F:CYS85 2.4 24.7 1.0 CB F:CYS82 3.1 25.6 1.0 CB F:CYS60 3.3 17.1 1.0 CB F:CYS62 3.4 21.9 1.0 CB F:CYS85 3.5 24.1 1.0 CA F:CYS62 3.5 25.0 1.0 N F:CYS85 3.7 25.3 1.0 N F:CYS62 3.8 22.2 1.0 CA F:CYS85 4.1 24.9 1.0 O F:CYS60 4.3 18.8 1.0 C F:CYS60 4.4 18.1 1.0 CA F:CYS60 4.5 17.2 1.0 CA F:CYS82 4.6 24.1 1.0 OG F:SER84 4.6 28.0 1.0 CB F:SER84 4.6 24.6 1.0 C F:ILE61 4.7 20.3 1.0 C F:SER84 4.7 25.5 1.0 O F:HOH2322 4.8 38.7 1.0 C F:CYS85 4.9 25.4 1.0 C F:CYS62 4.9 28.4 1.0 CG1 F:ILE70 4.9 17.5 1.0 CB F:ILE70 4.9 18.3 1.0 N F:ILE61 5.0 19.3 1.0

Zinc binding site 2 out of 2 in the Bovine Heart Cytochrome C Oxidase at the Fully Oxidized State (1-S X- Ray Exposure Dataset)


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Bovine Heart Cytochrome C Oxidase at the Fully Oxidized State (1-S X- Ray Exposure Dataset) within 5.0Å range: probe atom residue distance (Å) B Occ S:Zn99 b:28.0 occ:1.00 SG S:CYS85 2.3 22.6 1.0 SG S:CYS62 2.4 31.6 1.0 SG S:CYS60 2.4 27.1 1.0 SG S:CYS82 2.4 26.0 1.0 CB S:CYS82 3.1 27.2 1.0 CB S:CYS60 3.2 23.9 1.0 CB S:CYS62 3.3 30.5 1.0 CB S:CYS85 3.5 24.6 1.0 CA S:CYS62 3.7 31.1 1.0 N S:CYS85 3.8 25.9 1.0 OG1 S:THR87 4.0 35.9 1.0 N S:CYS62 4.0 28.7 1.0 CA S:CYS85 4.2 24.7 1.0 CA S:CYS60 4.5 24.4 1.0 C S:CYS60 4.5 24.1 1.0 O S:CYS60 4.5 23.6 1.0 CA S:CYS82 4.5 27.4 1.0 OG S:SER84 4.7 32.6 1.0 C S:SER84 4.7 27.6 1.0 CB S:SER84 4.7 29.2 1.0 C S:CYS85 4.8 25.7 1.0 CG1 S:ILE70 4.8 18.1 1.0 CB S:ILE70 4.9 20.4 1.0 C S:ILE61 4.9 27.1 1.0 N S:SER84 5.0 29.2 1.0 O S:HOH3322 5.0 46.8 1.0

H.Aoyama, K.Muramoto, K.Shinzawa-Itoh, K.Hirata, E.Yamashita, T.Tsukihara, T.Ogura, S.Yoshikawa. A Peroxide Bridge Between Fe and Cu Ions in the O2 Reduction Site of Fully Oxidized Cytochrome C Oxidase Could Suppress the Proton Pump Proc.Natl.Acad.Sci.Usa V. 106 2165 2009.
ISSN: ISSN 0027-8424
PubMed: 19164527
DOI: 10.1073/PNAS.0806391106