Zinc in PDB 2z8z: Crystal Structure of A Platinum-Bound S445C Mutant of Pseudomonas Sp. MIS38 Lipase

All present enzymatic activity of Crystal Structure of A Platinum-Bound S445C Mutant of Pseudomonas Sp. MIS38 Lipase:
3.1.1.3;

The structure of Crystal Structure of A Platinum-Bound S445C Mutant of Pseudomonas Sp. MIS38 Lipase, PDB code: 2z8z was solved by C.Angkawidjaja, D.J.You, H.Matsumura, Y.Koga, K.Takano, S.Kanaya, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	32.12 / 1.80
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	49.879, 84.404, 87.012, 90.00, 96.50, 90.00
R / Rfree (%)	17.4 / 20.5

The structure of Crystal Structure of A Platinum-Bound S445C Mutant of Pseudomonas Sp. MIS38 Lipase also contains other interesting chemical elements:

Platinum	(Pt)	1 atom
Calcium	(Ca)	11 atoms

The binding sites of Zinc atom in the Crystal Structure of A Platinum-Bound S445C Mutant of Pseudomonas Sp. MIS38 Lipase (pdb code 2z8z). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Crystal Structure of A Platinum-Bound S445C Mutant of Pseudomonas Sp. MIS38 Lipase, PDB code: 2z8z:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Crystal Structure of A Platinum-Bound S445C Mutant of Pseudomonas Sp. MIS38 Lipase


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Crystal Structure of A Platinum-Bound S445C Mutant of Pseudomonas Sp. MIS38 Lipase within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn629 b:23.8 occ:1.00 ND1 A:HIS132 2.0 23.4 1.0 OD2 A:ASP128 2.0 20.5 1.0 CG A:ASP128 2.9 22.0 1.0 CG A:HIS132 3.0 24.0 1.0 CE1 A:HIS132 3.0 25.8 1.0 OD1 A:ASP128 3.2 21.6 1.0 CB A:HIS132 3.3 20.1 1.0 NE2 A:HIS132 4.1 26.4 1.0 CD2 A:HIS132 4.1 25.4 1.0 CB A:ASP128 4.2 21.4 1.0 CB A:GLN130 4.3 25.6 1.0 CA A:HIS132 4.6 20.6 1.0 CG A:GLN130 4.7 27.0 1.0 N A:HIS132 4.8 21.1 1.0

Zinc binding site 2 out of 2 in the Crystal Structure of A Platinum-Bound S445C Mutant of Pseudomonas Sp. MIS38 Lipase


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Crystal Structure of A Platinum-Bound S445C Mutant of Pseudomonas Sp. MIS38 Lipase within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn630 b:86.3 occ:1.00 NE2 A:HIS517 2.1 18.9 1.0 OE2 A:GLU537 2.1 22.9 1.0 CD A:GLU537 2.7 22.6 1.0 OE1 A:GLU537 2.7 23.5 1.0 CE1 A:HIS517 3.0 20.6 1.0 CD2 A:HIS517 3.1 20.2 1.0 CG1 A:ILE519 4.1 20.1 1.0 ND1 A:HIS517 4.1 19.0 1.0 CG A:GLU537 4.1 18.4 1.0 CG A:HIS517 4.2 21.2 1.0 CD1 A:ILE519 4.3 20.4 1.0 CD2 A:LEU535 4.6 21.2 1.0 O A:HOH1082 5.0 43.5 1.0

C.Angkawidjaja, D.J.You, H.Matsumura, K.Kuwahara, Y.Koga, K.Takano, S.Kanaya. Crystal Structure of A Family I.3 Lipase From Pseudomonas Sp. MIS38 in A Closed Conformation Febs Lett. V. 581 5060 2007.
ISSN: ISSN 0014-5793
PubMed: 17923123
DOI: 10.1016/J.FEBSLET.2007.09.048