Zinc in PDB 2z8x: Crystal Structure of Extracellular Lipase From Pseudomonas Sp. MIS38

All present enzymatic activity of Crystal Structure of Extracellular Lipase From Pseudomonas Sp. MIS38:
3.1.1.3;

The structure of Crystal Structure of Extracellular Lipase From Pseudomonas Sp. MIS38, PDB code: 2z8x was solved by C.Angkawidjaja, D.J.You, H.Matsumura, Y.Koga, K.Takano, S.Kanaya, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	37.88 / 1.48
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	49.972, 84.300, 86.849, 90.00, 96.32, 90.00
R / Rfree (%)	17.6 / 19.4

The structure of Crystal Structure of Extracellular Lipase From Pseudomonas Sp. MIS38 also contains other interesting chemical elements:

Calcium

(Ca)

10 atoms

The binding sites of Zinc atom in the Crystal Structure of Extracellular Lipase From Pseudomonas Sp. MIS38 (pdb code 2z8x). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Crystal Structure of Extracellular Lipase From Pseudomonas Sp. MIS38, PDB code: 2z8x:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Crystal Structure of Extracellular Lipase From Pseudomonas Sp. MIS38


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Crystal Structure of Extracellular Lipase From Pseudomonas Sp. MIS38 within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn628 b:0.7 occ:1.00 OD2 A:ASP128 1.9 9.9 1.0 ND1 A:HIS132 2.1 13.3 1.0 CG A:ASP128 2.8 13.4 1.0 CE1 A:HIS132 3.0 11.8 1.0 OD1 A:ASP128 3.1 12.5 1.0 CG A:HIS132 3.1 11.8 1.0 CB A:HIS132 3.4 12.1 1.0 CB A:ASP128 4.1 13.8 1.0 CB A:GLN130 4.1 16.2 1.0 NE2 A:HIS132 4.1 13.4 1.0 CD2 A:HIS132 4.2 13.2 1.0 CG A:GLN130 4.6 17.6 1.0 CA A:HIS132 4.7 11.6 1.0 N A:HIS132 4.7 12.5 1.0

Zinc binding site 2 out of 2 in the Crystal Structure of Extracellular Lipase From Pseudomonas Sp. MIS38


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Crystal Structure of Extracellular Lipase From Pseudomonas Sp. MIS38 within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn629 b:11.1 occ:1.00 OE2 A:GLU537 2.0 10.4 1.0 NE2 A:HIS517 2.1 10.7 1.0 CD A:GLU537 2.7 11.2 1.0 OE1 A:GLU537 2.9 13.7 1.0 CE1 A:HIS517 3.0 11.6 1.0 CD2 A:HIS517 3.1 11.1 1.0 ND1 A:HIS517 4.1 9.7 1.0 CG A:GLU537 4.2 10.7 1.0 CG A:HIS517 4.2 10.1 1.0 CG1 A:ILE519 4.4 9.8 1.0 CD2 A:LEU535 4.4 12.6 1.0 CD1 A:ILE519 4.6 10.7 1.0

C.Angkawidjaja, D.J.You, H.Matsumura, K.Kuwahara, Y.Koga, K.Takano, S.Kanaya. Crystal Structure of A Family I.3 Lipase From Pseudomonas Sp. MIS38 in A Closed Conformation Febs Lett. V. 581 5060 2007.
ISSN: ISSN 0014-5793
PubMed: 17923123
DOI: 10.1016/J.FEBSLET.2007.09.048