Zinc in PDB 2y20: The Mechanisms of Hamp-Mediated Signaling in Transmembrane Receptors - the A291I Mutant

The structure of The Mechanisms of Hamp-Mediated Signaling in Transmembrane Receptors - the A291I Mutant, PDB code: 2y20 was solved by K.Zeth, H.U.Ferris, M.Hulko, A.N.Lupas, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	29.81 / 1.65
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	48.490, 64.760, 93.110, 90.00, 90.00, 90.00
R / Rfree (%)	18.9 / 23.6

The binding sites of Zinc atom in the The Mechanisms of Hamp-Mediated Signaling in Transmembrane Receptors - the A291I Mutant (pdb code 2y20). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 9 binding sites of Zinc where determined in the The Mechanisms of Hamp-Mediated Signaling in Transmembrane Receptors - the A291I Mutant, PDB code: 2y20:
Jump to Zinc binding site number: 1; 2; 3; 4; 5; 6; 7; 8; 9;

Zinc binding site 1 out of 9 in the The Mechanisms of Hamp-Mediated Signaling in Transmembrane Receptors - the A291I Mutant


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of The Mechanisms of Hamp-Mediated Signaling in Transmembrane Receptors - the A291I Mutant within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn1331 b:29.4 occ:1.00 O A:HOH2007 1.9 18.5 1.0 OE1 A:GLU286 2.0 25.7 1.0 O A:HOH2004 2.0 18.1 1.0 NE2 A:HIS305 2.0 26.0 1.0 O A:HOH2003 2.5 20.5 1.0 CD A:GLU286 2.7 29.3 1.0 OE2 A:GLU286 2.8 30.7 1.0 CE1 A:HIS305 3.0 29.4 1.0 CD2 A:HIS305 3.0 25.4 1.0 ND1 A:HIS305 4.1 26.7 1.0 CG A:GLU286 4.1 20.7 1.0 CG A:HIS305 4.1 23.0 1.0 NE A:ARG282 4.2 33.7 1.0 CB A:GLU286 4.6 18.3 1.0 CG A:ARG282 4.7 26.8 1.0 CA A:PRO283 4.7 19.4 1.0 CB A:PRO283 4.8 21.5 1.0 CD A:ARG282 4.9 34.2 1.0 CB A:PRO304 4.9 20.9 1.0 NH2 A:ARG282 4.9 43.6 1.0 CZ A:ARG282 5.0 42.5 1.0

Zinc binding site 2 out of 9 in the The Mechanisms of Hamp-Mediated Signaling in Transmembrane Receptors - the A291I Mutant


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of The Mechanisms of Hamp-Mediated Signaling in Transmembrane Receptors - the A291I Mutant within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn1332 b:28.2 occ:1.00 O B:HOH2005 1.5 26.3 1.0 NE2 B:HIS305 2.0 24.5 1.0 OE1 B:GLU286 2.0 20.0 1.0 O B:HOH2003 2.1 20.5 1.0 O B:HOH2002 2.4 21.2 1.0 CD B:GLU286 2.7 22.9 1.0 OE2 B:GLU286 2.9 26.5 1.0 CE1 B:HIS305 3.0 27.9 1.0 CD2 B:HIS305 3.0 23.9 1.0 ND1 B:HIS305 4.1 25.7 1.0 CG B:HIS305 4.1 21.6 1.0 NE B:ARG282 4.1 33.1 1.0 CG B:GLU286 4.2 19.2 1.0 O B:HOH2030 4.2 21.7 1.0 CA B:PRO283 4.6 17.2 1.0 CB B:GLU286 4.7 15.9 1.0 CG B:ARG282 4.7 20.5 1.0 CB B:PRO304 4.7 20.2 1.0 CB B:PRO283 4.7 18.2 1.0 NH2 B:ARG282 4.7 41.2 1.0 CZ B:ARG282 4.8 35.1 1.0 CD B:ARG282 4.8 23.8 1.0 CG B:PRO304 5.0 19.1 1.0 N B:PRO283 5.0 16.7 1.0

Zinc binding site 3 out of 9 in the The Mechanisms of Hamp-Mediated Signaling in Transmembrane Receptors - the A291I Mutant


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 3 of The Mechanisms of Hamp-Mediated Signaling in Transmembrane Receptors - the A291I Mutant within 5.0Å range: probe atom residue distance (Å) B Occ C:Zn1331 b:37.2 occ:1.00 NE2 C:HIS305 1.8 32.5 1.0 OE1 C:GLU286 1.9 31.3 1.0 O C:HOH2004 2.3 11.9 1.0 CE1 C:HIS305 2.6 35.5 1.0 O C:HOH2009 2.6 15.7 1.0 CD C:GLU286 2.9 28.9 1.0 CD2 C:HIS305 3.0 25.9 1.0 OE2 C:GLU286 3.2 29.7 1.0 ND1 C:HIS305 3.8 30.4 1.0 CG C:HIS305 4.0 26.5 1.0 CG C:GLU286 4.3 21.6 1.0 CG C:ARG282 4.6 18.5 1.0 CB C:GLU286 4.7 19.1 1.0 CB C:PRO304 4.7 28.2 1.0 CA C:PRO283 4.7 17.9 1.0 CD C:ARG282 4.7 27.0 1.0 CG C:PRO304 4.8 25.6 1.0 NE C:ARG282 4.9 32.0 1.0 CB C:PRO283 4.9 18.3 1.0

Zinc binding site 4 out of 9 in the The Mechanisms of Hamp-Mediated Signaling in Transmembrane Receptors - the A291I Mutant


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 4 of The Mechanisms of Hamp-Mediated Signaling in Transmembrane Receptors - the A291I Mutant within 5.0Å range: probe atom residue distance (Å) B Occ C:Zn1332 b:26.8 occ:1.00 NE2 C:HIS276 2.0 24.2 1.0 OE2 D:GLU311 2.0 30.5 1.0 CD D:GLU311 2.6 29.5 1.0 OE1 D:GLU311 2.7 34.3 1.0 CE1 C:HIS276 2.8 28.8 1.0 CD2 C:HIS276 3.1 23.0 1.0 OG1 C:THR281 3.1 22.1 1.0 ND1 C:HIS276 4.0 25.3 1.0 CG D:GLU311 4.1 28.6 1.0 CG C:HIS276 4.1 26.3 1.0 CB C:THR281 4.3 19.8 1.0 OD2 D:ASP310 4.4 47.6 1.0 CD1 D:ILE280 4.5 38.8 1.0 CG2 C:THR281 4.5 18.9 1.0 CA C:THR281 4.7 18.1 1.0 CG2 C:ILE280 4.8 26.4 1.0 N C:THR281 4.9 18.6 1.0

Zinc binding site 5 out of 9 in the The Mechanisms of Hamp-Mediated Signaling in Transmembrane Receptors - the A291I Mutant


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 5 of The Mechanisms of Hamp-Mediated Signaling in Transmembrane Receptors - the A291I Mutant within 5.0Å range: probe atom residue distance (Å) B Occ D:Zn1331 b:44.8 occ:1.00 OE1 D:GLU286 2.0 31.6 1.0 O D:HOH2004 2.2 24.4 1.0 NE2 D:HIS305 2.2 49.5 1.0 O D:HOH2010 2.3 23.7 1.0 CD D:GLU286 2.8 33.4 1.0 OE2 D:GLU286 3.0 41.2 1.0 CE1 D:HIS305 3.1 52.4 1.0 CD2 D:HIS305 3.3 43.8 1.0 CG D:GLU286 4.2 24.1 1.0 ND1 D:HIS305 4.2 48.1 1.0 CG D:HIS305 4.4 43.0 1.0 CA D:PRO283 4.5 22.5 1.0 CB D:GLU286 4.6 22.0 1.0 CB D:PRO283 4.6 24.2 1.0 CG D:ARG282 4.7 35.6 1.0 N D:PRO283 5.0 24.3 1.0 CB D:PRO304 5.0 41.4 1.0

Zinc binding site 6 out of 9 in the The Mechanisms of Hamp-Mediated Signaling in Transmembrane Receptors - the A291I Mutant


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 6 of The Mechanisms of Hamp-Mediated Signaling in Transmembrane Receptors - the A291I Mutant within 5.0Å range: probe atom residue distance (Å) B Occ E:Zn1331 b:23.1 occ:1.00 O E:HOH2009 1.8 30.2 1.0 OE1 E:GLU286 1.8 20.1 1.0 NE2 E:HIS305 2.0 19.5 1.0 O E:HOH2006 2.1 11.5 1.0 O E:HOH2005 2.5 12.6 1.0 CD E:GLU286 2.7 21.0 1.0 CE1 E:HIS305 2.9 21.5 1.0 OE2 E:GLU286 2.9 22.2 1.0 CD2 E:HIS305 3.1 20.8 1.0 ND1 E:HIS305 4.1 18.3 1.0 CG E:GLU286 4.1 18.1 1.0 CG E:HIS305 4.2 16.7 1.0 NE E:ARG282 4.3 26.4 1.0 CB E:GLU286 4.5 15.4 1.0 CA E:PRO283 4.7 14.3 1.0 CG E:ARG282 4.7 22.4 1.0 CB E:PRO304 4.7 21.9 1.0 NH2 E:ARG282 4.8 38.6 1.0 CZ E:ARG282 4.8 34.4 1.0 CD E:ARG282 4.9 25.6 1.0 CB E:PRO283 5.0 16.0 1.0

Zinc binding site 7 out of 9 in the The Mechanisms of Hamp-Mediated Signaling in Transmembrane Receptors - the A291I Mutant


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 7 of The Mechanisms of Hamp-Mediated Signaling in Transmembrane Receptors - the A291I Mutant within 5.0Å range: probe atom residue distance (Å) B Occ E:Zn1332 b:26.3 occ:1.00 NE2 E:HIS276 2.0 23.4 1.0 OE2 F:GLU311 2.3 36.0 1.0 OE1 F:GLU311 2.3 36.6 1.0 CD F:GLU311 2.4 38.9 1.0 CD2 E:HIS276 2.9 23.5 1.0 CE1 E:HIS276 3.1 25.7 1.0 CG F:GLU311 3.7 37.9 1.0 OG1 E:THR281 3.8 27.6 1.0 CG E:HIS276 4.1 24.9 1.0 ND1 E:HIS276 4.1 27.0 1.0 OD2 F:ASP310 4.2 34.6 1.0 CD1 F:ILE280 4.4 28.4 1.0 CB F:ASP310 4.6 30.1 1.0 CG F:ASP310 4.7 29.4 1.0 CE C:MET277 4.7 29.1 1.0 CB F:GLU311 4.8 27.2 1.0 CB E:THR281 4.9 24.2 1.0 N F:GLU311 4.9 25.6 1.0

Zinc binding site 8 out of 9 in the The Mechanisms of Hamp-Mediated Signaling in Transmembrane Receptors - the A291I Mutant


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 8 of The Mechanisms of Hamp-Mediated Signaling in Transmembrane Receptors - the A291I Mutant within 5.0Å range: probe atom residue distance (Å) B Occ F:Zn1331 b:21.4 occ:1.00 O F:HOH2005 2.0 8.0 1.0 OE1 F:GLU286 2.0 18.7 1.0 NE2 F:HIS305 2.0 18.2 1.0 CD F:GLU286 2.8 21.7 1.0 OE2 F:GLU286 2.9 22.6 1.0 CE1 F:HIS305 2.9 23.5 1.0 CD2 F:HIS305 3.1 23.9 1.0 ND1 F:HIS305 4.1 24.7 1.0 CG F:HIS305 4.2 19.9 1.0 CG F:GLU286 4.2 19.9 1.0 NE F:ARG282 4.4 30.0 1.0 CA F:PRO283 4.6 19.4 1.0 CB F:GLU286 4.6 19.3 1.0 CB F:PRO304 4.7 21.2 1.0 CB F:PRO283 4.7 19.7 1.0 CG F:ARG282 4.7 18.6 1.0 CD F:ARG282 4.9 23.2 1.0 CG F:PRO304 4.9 19.2 1.0 CZ F:ARG282 4.9 38.9 1.0 NH2 F:ARG282 5.0 43.5 1.0

Zinc binding site 9 out of 9 in the The Mechanisms of Hamp-Mediated Signaling in Transmembrane Receptors - the A291I Mutant


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 9 of The Mechanisms of Hamp-Mediated Signaling in Transmembrane Receptors - the A291I Mutant within 5.0Å range: probe atom residue distance (Å) B Occ F:Zn1332 b:41.8 occ:1.00 OE2 E:GLU311 2.0 28.3 1.0 NE2 F:HIS276 2.1 30.7 1.0 O E:HOH2020 2.2 19.6 1.0 CD E:GLU311 2.7 27.3 1.0 OG1 F:THR281 2.7 22.8 1.0 OE1 E:GLU311 2.7 27.0 1.0 CD2 F:HIS276 3.0 32.8 1.0 CE1 F:HIS276 3.2 34.2 1.0 O F:HOH2004 3.6 22.9 1.0 CB F:THR281 4.0 22.7 1.0 CG F:HIS276 4.2 33.3 1.0 ND1 F:HIS276 4.2 35.3 1.0 CG E:GLU311 4.2 19.4 1.0 CG2 F:THR281 4.4 24.1 1.0 CA F:THR281 4.4 20.7 1.0 CG2 F:ILE280 4.5 24.2 1.0 CD1 E:ILE280 4.5 28.9 1.0 N F:THR281 4.6 22.2 1.0

H.U.Ferris, S.Dunin-Horkawicz, L.G.Mondejar, M.Hulko, K.Hantke, J.Martin, J.E.Schultz, K.Zeth, A.N.Lupas, M.Coles. The Mechanisms of Hamp-Mediated Signaling in Transmembrane Receptors. Structure V. 19 378 2011.
ISSN: ISSN 0969-2126
PubMed: 21397188
DOI: 10.1016/J.STR.2011.01.006