Zinc in PDB 2y20: The Mechanisms of Hamp-Mediated Signaling in Transmembrane Receptors - the A291I Mutant
Protein crystallography data
The structure of The Mechanisms of Hamp-Mediated Signaling in Transmembrane Receptors - the A291I Mutant, PDB code: 2y20
was solved by
K.Zeth,
H.U.Ferris,
M.Hulko,
A.N.Lupas,
with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:
Resolution Low / High (Å)
|
29.81 /
1.65
|
Space group
|
P 21 21 21
|
Cell size a, b, c (Å), α, β, γ (°)
|
48.490,
64.760,
93.110,
90.00,
90.00,
90.00
|
R / Rfree (%)
|
18.9 /
23.6
|
Zinc Binding Sites:
The binding sites of Zinc atom in the The Mechanisms of Hamp-Mediated Signaling in Transmembrane Receptors - the A291I Mutant
(pdb code 2y20). This binding sites where shown within
5.0 Angstroms radius around Zinc atom.
In total 9 binding sites of Zinc where determined in the
The Mechanisms of Hamp-Mediated Signaling in Transmembrane Receptors - the A291I Mutant, PDB code: 2y20:
Jump to Zinc binding site number:
1;
2;
3;
4;
5;
6;
7;
8;
9;
Zinc binding site 1 out
of 9 in 2y20
Go back to
Zinc Binding Sites List in 2y20
Zinc binding site 1 out
of 9 in the The Mechanisms of Hamp-Mediated Signaling in Transmembrane Receptors - the A291I Mutant
Mono view
Stereo pair view
|
A full contact list of Zinc with other atoms in the Zn binding
site number 1 of The Mechanisms of Hamp-Mediated Signaling in Transmembrane Receptors - the A291I Mutant within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Zn1331
b:29.4
occ:1.00
|
O
|
A:HOH2007
|
1.9
|
18.5
|
1.0
|
OE1
|
A:GLU286
|
2.0
|
25.7
|
1.0
|
O
|
A:HOH2004
|
2.0
|
18.1
|
1.0
|
NE2
|
A:HIS305
|
2.0
|
26.0
|
1.0
|
O
|
A:HOH2003
|
2.5
|
20.5
|
1.0
|
CD
|
A:GLU286
|
2.7
|
29.3
|
1.0
|
OE2
|
A:GLU286
|
2.8
|
30.7
|
1.0
|
CE1
|
A:HIS305
|
3.0
|
29.4
|
1.0
|
CD2
|
A:HIS305
|
3.0
|
25.4
|
1.0
|
ND1
|
A:HIS305
|
4.1
|
26.7
|
1.0
|
CG
|
A:GLU286
|
4.1
|
20.7
|
1.0
|
CG
|
A:HIS305
|
4.1
|
23.0
|
1.0
|
NE
|
A:ARG282
|
4.2
|
33.7
|
1.0
|
CB
|
A:GLU286
|
4.6
|
18.3
|
1.0
|
CG
|
A:ARG282
|
4.7
|
26.8
|
1.0
|
CA
|
A:PRO283
|
4.7
|
19.4
|
1.0
|
CB
|
A:PRO283
|
4.8
|
21.5
|
1.0
|
CD
|
A:ARG282
|
4.9
|
34.2
|
1.0
|
CB
|
A:PRO304
|
4.9
|
20.9
|
1.0
|
NH2
|
A:ARG282
|
4.9
|
43.6
|
1.0
|
CZ
|
A:ARG282
|
5.0
|
42.5
|
1.0
|
|
Zinc binding site 2 out
of 9 in 2y20
Go back to
Zinc Binding Sites List in 2y20
Zinc binding site 2 out
of 9 in the The Mechanisms of Hamp-Mediated Signaling in Transmembrane Receptors - the A291I Mutant
Mono view
Stereo pair view
|
A full contact list of Zinc with other atoms in the Zn binding
site number 2 of The Mechanisms of Hamp-Mediated Signaling in Transmembrane Receptors - the A291I Mutant within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
B:Zn1332
b:28.2
occ:1.00
|
O
|
B:HOH2005
|
1.5
|
26.3
|
1.0
|
NE2
|
B:HIS305
|
2.0
|
24.5
|
1.0
|
OE1
|
B:GLU286
|
2.0
|
20.0
|
1.0
|
O
|
B:HOH2003
|
2.1
|
20.5
|
1.0
|
O
|
B:HOH2002
|
2.4
|
21.2
|
1.0
|
CD
|
B:GLU286
|
2.7
|
22.9
|
1.0
|
OE2
|
B:GLU286
|
2.9
|
26.5
|
1.0
|
CE1
|
B:HIS305
|
3.0
|
27.9
|
1.0
|
CD2
|
B:HIS305
|
3.0
|
23.9
|
1.0
|
ND1
|
B:HIS305
|
4.1
|
25.7
|
1.0
|
CG
|
B:HIS305
|
4.1
|
21.6
|
1.0
|
NE
|
B:ARG282
|
4.1
|
33.1
|
1.0
|
CG
|
B:GLU286
|
4.2
|
19.2
|
1.0
|
O
|
B:HOH2030
|
4.2
|
21.7
|
1.0
|
CA
|
B:PRO283
|
4.6
|
17.2
|
1.0
|
CB
|
B:GLU286
|
4.7
|
15.9
|
1.0
|
CG
|
B:ARG282
|
4.7
|
20.5
|
1.0
|
CB
|
B:PRO304
|
4.7
|
20.2
|
1.0
|
CB
|
B:PRO283
|
4.7
|
18.2
|
1.0
|
NH2
|
B:ARG282
|
4.7
|
41.2
|
1.0
|
CZ
|
B:ARG282
|
4.8
|
35.1
|
1.0
|
CD
|
B:ARG282
|
4.8
|
23.8
|
1.0
|
CG
|
B:PRO304
|
5.0
|
19.1
|
1.0
|
N
|
B:PRO283
|
5.0
|
16.7
|
1.0
|
|
Zinc binding site 3 out
of 9 in 2y20
Go back to
Zinc Binding Sites List in 2y20
Zinc binding site 3 out
of 9 in the The Mechanisms of Hamp-Mediated Signaling in Transmembrane Receptors - the A291I Mutant
Mono view
Stereo pair view
|
A full contact list of Zinc with other atoms in the Zn binding
site number 3 of The Mechanisms of Hamp-Mediated Signaling in Transmembrane Receptors - the A291I Mutant within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
C:Zn1331
b:37.2
occ:1.00
|
NE2
|
C:HIS305
|
1.8
|
32.5
|
1.0
|
OE1
|
C:GLU286
|
1.9
|
31.3
|
1.0
|
O
|
C:HOH2004
|
2.3
|
11.9
|
1.0
|
CE1
|
C:HIS305
|
2.6
|
35.5
|
1.0
|
O
|
C:HOH2009
|
2.6
|
15.7
|
1.0
|
CD
|
C:GLU286
|
2.9
|
28.9
|
1.0
|
CD2
|
C:HIS305
|
3.0
|
25.9
|
1.0
|
OE2
|
C:GLU286
|
3.2
|
29.7
|
1.0
|
ND1
|
C:HIS305
|
3.8
|
30.4
|
1.0
|
CG
|
C:HIS305
|
4.0
|
26.5
|
1.0
|
CG
|
C:GLU286
|
4.3
|
21.6
|
1.0
|
CG
|
C:ARG282
|
4.6
|
18.5
|
1.0
|
CB
|
C:GLU286
|
4.7
|
19.1
|
1.0
|
CB
|
C:PRO304
|
4.7
|
28.2
|
1.0
|
CA
|
C:PRO283
|
4.7
|
17.9
|
1.0
|
CD
|
C:ARG282
|
4.7
|
27.0
|
1.0
|
CG
|
C:PRO304
|
4.8
|
25.6
|
1.0
|
NE
|
C:ARG282
|
4.9
|
32.0
|
1.0
|
CB
|
C:PRO283
|
4.9
|
18.3
|
1.0
|
|
Zinc binding site 4 out
of 9 in 2y20
Go back to
Zinc Binding Sites List in 2y20
Zinc binding site 4 out
of 9 in the The Mechanisms of Hamp-Mediated Signaling in Transmembrane Receptors - the A291I Mutant
Mono view
Stereo pair view
|
A full contact list of Zinc with other atoms in the Zn binding
site number 4 of The Mechanisms of Hamp-Mediated Signaling in Transmembrane Receptors - the A291I Mutant within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
C:Zn1332
b:26.8
occ:1.00
|
NE2
|
C:HIS276
|
2.0
|
24.2
|
1.0
|
OE2
|
D:GLU311
|
2.0
|
30.5
|
1.0
|
CD
|
D:GLU311
|
2.6
|
29.5
|
1.0
|
OE1
|
D:GLU311
|
2.7
|
34.3
|
1.0
|
CE1
|
C:HIS276
|
2.8
|
28.8
|
1.0
|
CD2
|
C:HIS276
|
3.1
|
23.0
|
1.0
|
OG1
|
C:THR281
|
3.1
|
22.1
|
1.0
|
ND1
|
C:HIS276
|
4.0
|
25.3
|
1.0
|
CG
|
D:GLU311
|
4.1
|
28.6
|
1.0
|
CG
|
C:HIS276
|
4.1
|
26.3
|
1.0
|
CB
|
C:THR281
|
4.3
|
19.8
|
1.0
|
OD2
|
D:ASP310
|
4.4
|
47.6
|
1.0
|
CD1
|
D:ILE280
|
4.5
|
38.8
|
1.0
|
CG2
|
C:THR281
|
4.5
|
18.9
|
1.0
|
CA
|
C:THR281
|
4.7
|
18.1
|
1.0
|
CG2
|
C:ILE280
|
4.8
|
26.4
|
1.0
|
N
|
C:THR281
|
4.9
|
18.6
|
1.0
|
|
Zinc binding site 5 out
of 9 in 2y20
Go back to
Zinc Binding Sites List in 2y20
Zinc binding site 5 out
of 9 in the The Mechanisms of Hamp-Mediated Signaling in Transmembrane Receptors - the A291I Mutant
Mono view
Stereo pair view
|
A full contact list of Zinc with other atoms in the Zn binding
site number 5 of The Mechanisms of Hamp-Mediated Signaling in Transmembrane Receptors - the A291I Mutant within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
D:Zn1331
b:44.8
occ:1.00
|
OE1
|
D:GLU286
|
2.0
|
31.6
|
1.0
|
O
|
D:HOH2004
|
2.2
|
24.4
|
1.0
|
NE2
|
D:HIS305
|
2.2
|
49.5
|
1.0
|
O
|
D:HOH2010
|
2.3
|
23.7
|
1.0
|
CD
|
D:GLU286
|
2.8
|
33.4
|
1.0
|
OE2
|
D:GLU286
|
3.0
|
41.2
|
1.0
|
CE1
|
D:HIS305
|
3.1
|
52.4
|
1.0
|
CD2
|
D:HIS305
|
3.3
|
43.8
|
1.0
|
CG
|
D:GLU286
|
4.2
|
24.1
|
1.0
|
ND1
|
D:HIS305
|
4.2
|
48.1
|
1.0
|
CG
|
D:HIS305
|
4.4
|
43.0
|
1.0
|
CA
|
D:PRO283
|
4.5
|
22.5
|
1.0
|
CB
|
D:GLU286
|
4.6
|
22.0
|
1.0
|
CB
|
D:PRO283
|
4.6
|
24.2
|
1.0
|
CG
|
D:ARG282
|
4.7
|
35.6
|
1.0
|
N
|
D:PRO283
|
5.0
|
24.3
|
1.0
|
CB
|
D:PRO304
|
5.0
|
41.4
|
1.0
|
|
Zinc binding site 6 out
of 9 in 2y20
Go back to
Zinc Binding Sites List in 2y20
Zinc binding site 6 out
of 9 in the The Mechanisms of Hamp-Mediated Signaling in Transmembrane Receptors - the A291I Mutant
Mono view
Stereo pair view
|
A full contact list of Zinc with other atoms in the Zn binding
site number 6 of The Mechanisms of Hamp-Mediated Signaling in Transmembrane Receptors - the A291I Mutant within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
E:Zn1331
b:23.1
occ:1.00
|
O
|
E:HOH2009
|
1.8
|
30.2
|
1.0
|
OE1
|
E:GLU286
|
1.8
|
20.1
|
1.0
|
NE2
|
E:HIS305
|
2.0
|
19.5
|
1.0
|
O
|
E:HOH2006
|
2.1
|
11.5
|
1.0
|
O
|
E:HOH2005
|
2.5
|
12.6
|
1.0
|
CD
|
E:GLU286
|
2.7
|
21.0
|
1.0
|
CE1
|
E:HIS305
|
2.9
|
21.5
|
1.0
|
OE2
|
E:GLU286
|
2.9
|
22.2
|
1.0
|
CD2
|
E:HIS305
|
3.1
|
20.8
|
1.0
|
ND1
|
E:HIS305
|
4.1
|
18.3
|
1.0
|
CG
|
E:GLU286
|
4.1
|
18.1
|
1.0
|
CG
|
E:HIS305
|
4.2
|
16.7
|
1.0
|
NE
|
E:ARG282
|
4.3
|
26.4
|
1.0
|
CB
|
E:GLU286
|
4.5
|
15.4
|
1.0
|
CA
|
E:PRO283
|
4.7
|
14.3
|
1.0
|
CG
|
E:ARG282
|
4.7
|
22.4
|
1.0
|
CB
|
E:PRO304
|
4.7
|
21.9
|
1.0
|
NH2
|
E:ARG282
|
4.8
|
38.6
|
1.0
|
CZ
|
E:ARG282
|
4.8
|
34.4
|
1.0
|
CD
|
E:ARG282
|
4.9
|
25.6
|
1.0
|
CB
|
E:PRO283
|
5.0
|
16.0
|
1.0
|
|
Zinc binding site 7 out
of 9 in 2y20
Go back to
Zinc Binding Sites List in 2y20
Zinc binding site 7 out
of 9 in the The Mechanisms of Hamp-Mediated Signaling in Transmembrane Receptors - the A291I Mutant
Mono view
Stereo pair view
|
A full contact list of Zinc with other atoms in the Zn binding
site number 7 of The Mechanisms of Hamp-Mediated Signaling in Transmembrane Receptors - the A291I Mutant within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
E:Zn1332
b:26.3
occ:1.00
|
NE2
|
E:HIS276
|
2.0
|
23.4
|
1.0
|
OE2
|
F:GLU311
|
2.3
|
36.0
|
1.0
|
OE1
|
F:GLU311
|
2.3
|
36.6
|
1.0
|
CD
|
F:GLU311
|
2.4
|
38.9
|
1.0
|
CD2
|
E:HIS276
|
2.9
|
23.5
|
1.0
|
CE1
|
E:HIS276
|
3.1
|
25.7
|
1.0
|
CG
|
F:GLU311
|
3.7
|
37.9
|
1.0
|
OG1
|
E:THR281
|
3.8
|
27.6
|
1.0
|
CG
|
E:HIS276
|
4.1
|
24.9
|
1.0
|
ND1
|
E:HIS276
|
4.1
|
27.0
|
1.0
|
OD2
|
F:ASP310
|
4.2
|
34.6
|
1.0
|
CD1
|
F:ILE280
|
4.4
|
28.4
|
1.0
|
CB
|
F:ASP310
|
4.6
|
30.1
|
1.0
|
CG
|
F:ASP310
|
4.7
|
29.4
|
1.0
|
CE
|
C:MET277
|
4.7
|
29.1
|
1.0
|
CB
|
F:GLU311
|
4.8
|
27.2
|
1.0
|
CB
|
E:THR281
|
4.9
|
24.2
|
1.0
|
N
|
F:GLU311
|
4.9
|
25.6
|
1.0
|
|
Zinc binding site 8 out
of 9 in 2y20
Go back to
Zinc Binding Sites List in 2y20
Zinc binding site 8 out
of 9 in the The Mechanisms of Hamp-Mediated Signaling in Transmembrane Receptors - the A291I Mutant
Mono view
Stereo pair view
|
A full contact list of Zinc with other atoms in the Zn binding
site number 8 of The Mechanisms of Hamp-Mediated Signaling in Transmembrane Receptors - the A291I Mutant within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
F:Zn1331
b:21.4
occ:1.00
|
O
|
F:HOH2005
|
2.0
|
8.0
|
1.0
|
OE1
|
F:GLU286
|
2.0
|
18.7
|
1.0
|
NE2
|
F:HIS305
|
2.0
|
18.2
|
1.0
|
CD
|
F:GLU286
|
2.8
|
21.7
|
1.0
|
OE2
|
F:GLU286
|
2.9
|
22.6
|
1.0
|
CE1
|
F:HIS305
|
2.9
|
23.5
|
1.0
|
CD2
|
F:HIS305
|
3.1
|
23.9
|
1.0
|
ND1
|
F:HIS305
|
4.1
|
24.7
|
1.0
|
CG
|
F:HIS305
|
4.2
|
19.9
|
1.0
|
CG
|
F:GLU286
|
4.2
|
19.9
|
1.0
|
NE
|
F:ARG282
|
4.4
|
30.0
|
1.0
|
CA
|
F:PRO283
|
4.6
|
19.4
|
1.0
|
CB
|
F:GLU286
|
4.6
|
19.3
|
1.0
|
CB
|
F:PRO304
|
4.7
|
21.2
|
1.0
|
CB
|
F:PRO283
|
4.7
|
19.7
|
1.0
|
CG
|
F:ARG282
|
4.7
|
18.6
|
1.0
|
CD
|
F:ARG282
|
4.9
|
23.2
|
1.0
|
CG
|
F:PRO304
|
4.9
|
19.2
|
1.0
|
CZ
|
F:ARG282
|
4.9
|
38.9
|
1.0
|
NH2
|
F:ARG282
|
5.0
|
43.5
|
1.0
|
|
Zinc binding site 9 out
of 9 in 2y20
Go back to
Zinc Binding Sites List in 2y20
Zinc binding site 9 out
of 9 in the The Mechanisms of Hamp-Mediated Signaling in Transmembrane Receptors - the A291I Mutant
Mono view
Stereo pair view
|
A full contact list of Zinc with other atoms in the Zn binding
site number 9 of The Mechanisms of Hamp-Mediated Signaling in Transmembrane Receptors - the A291I Mutant within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
F:Zn1332
b:41.8
occ:1.00
|
OE2
|
E:GLU311
|
2.0
|
28.3
|
1.0
|
NE2
|
F:HIS276
|
2.1
|
30.7
|
1.0
|
O
|
E:HOH2020
|
2.2
|
19.6
|
1.0
|
CD
|
E:GLU311
|
2.7
|
27.3
|
1.0
|
OG1
|
F:THR281
|
2.7
|
22.8
|
1.0
|
OE1
|
E:GLU311
|
2.7
|
27.0
|
1.0
|
CD2
|
F:HIS276
|
3.0
|
32.8
|
1.0
|
CE1
|
F:HIS276
|
3.2
|
34.2
|
1.0
|
O
|
F:HOH2004
|
3.6
|
22.9
|
1.0
|
CB
|
F:THR281
|
4.0
|
22.7
|
1.0
|
CG
|
F:HIS276
|
4.2
|
33.3
|
1.0
|
ND1
|
F:HIS276
|
4.2
|
35.3
|
1.0
|
CG
|
E:GLU311
|
4.2
|
19.4
|
1.0
|
CG2
|
F:THR281
|
4.4
|
24.1
|
1.0
|
CA
|
F:THR281
|
4.4
|
20.7
|
1.0
|
CG2
|
F:ILE280
|
4.5
|
24.2
|
1.0
|
CD1
|
E:ILE280
|
4.5
|
28.9
|
1.0
|
N
|
F:THR281
|
4.6
|
22.2
|
1.0
|
|
Reference:
H.U.Ferris,
S.Dunin-Horkawicz,
L.G.Mondejar,
M.Hulko,
K.Hantke,
J.Martin,
J.E.Schultz,
K.Zeth,
A.N.Lupas,
M.Coles.
The Mechanisms of Hamp-Mediated Signaling in Transmembrane Receptors. Structure V. 19 378 2011.
ISSN: ISSN 0969-2126
PubMed: 21397188
DOI: 10.1016/J.STR.2011.01.006
Page generated: Thu Oct 17 05:39:34 2024
|