Atomistry » Zinc » PDB 2xjy-2xxg » 2xxg
Atomistry »
  Zinc »
    PDB 2xjy-2xxg »
      2xxg »

Zinc in PDB 2xxg: Structure of the N90S Mutant of Nitrite Reductase From Alcaligenes Xylosoxidans

Protein crystallography data

The structure of Structure of the N90S Mutant of Nitrite Reductase From Alcaligenes Xylosoxidans, PDB code: 2xxg was solved by M.A.Hough, R.R.Eady, S.S.Hasnain, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 29.16 / 1.60
Space group H 3
Cell size a, b, c (Å), α, β, γ (°) 89.552, 89.552, 287.678, 90.00, 90.00, 120.00
R / Rfree (%) 16.75 / 21.074

Other elements in 2xxg:

The structure of Structure of the N90S Mutant of Nitrite Reductase From Alcaligenes Xylosoxidans also contains other interesting chemical elements:

Copper (Cu) 4 atoms

Zinc Binding Sites:

The binding sites of Zinc atom in the Structure of the N90S Mutant of Nitrite Reductase From Alcaligenes Xylosoxidans (pdb code 2xxg). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 4 binding sites of Zinc where determined in the Structure of the N90S Mutant of Nitrite Reductase From Alcaligenes Xylosoxidans, PDB code: 2xxg:
Jump to Zinc binding site number: 1; 2; 3; 4;

Zinc binding site 1 out of 4 in 2xxg

Go back to Zinc Binding Sites List in 2xxg
Zinc binding site 1 out of 4 in the Structure of the N90S Mutant of Nitrite Reductase From Alcaligenes Xylosoxidans


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Structure of the N90S Mutant of Nitrite Reductase From Alcaligenes Xylosoxidans within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Zn1339

b:24.8
occ:1.00
OE2 C:GLU195 1.9 26.9 1.0
NE2 A:HIS165 2.0 21.4 1.0
O A:HOH2331 2.1 32.2 1.0
OD2 A:ASP167 2.2 19.4 1.0
OD1 A:ASP167 2.5 22.4 1.0
CG A:ASP167 2.6 20.7 1.0
CD C:GLU195 2.7 24.7 1.0
CE1 A:HIS165 2.9 19.8 1.0
OE1 C:GLU195 3.0 25.3 1.0
CD2 A:HIS165 3.0 19.9 1.0
O C:HOH2212 3.7 34.1 1.0
OG1 A:THR234 3.9 17.3 1.0
ND1 A:HIS165 4.0 19.1 1.0
O C:HOH2206 4.0 29.1 1.0
O A:HOH2337 4.1 32.8 1.0
CG C:GLU195 4.1 22.9 1.0
CG A:HIS165 4.1 18.9 1.0
CB A:ASP167 4.1 20.2 1.0
CB A:THR234 4.3 17.6 1.0
CB C:ALA191 4.5 20.0 1.0
N A:THR234 4.5 18.7 1.0
N A:ASP167 4.6 19.7 1.0
O A:GLY232 4.8 23.2 1.0
CA A:ASP167 4.9 20.9 1.0

Zinc binding site 2 out of 4 in 2xxg

Go back to Zinc Binding Sites List in 2xxg
Zinc binding site 2 out of 4 in the Structure of the N90S Mutant of Nitrite Reductase From Alcaligenes Xylosoxidans


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Structure of the N90S Mutant of Nitrite Reductase From Alcaligenes Xylosoxidans within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Zn1341

b:39.2
occ:0.30
O A:HOH2332 1.6 63.6 1.0
O A:HOH2333 2.0 59.6 1.0
NE2 A:HIS8 2.0 36.0 1.0
CD2 A:HIS8 2.8 35.3 1.0
CE1 A:HIS8 3.0 35.2 1.0
O A:LEU6 3.5 34.7 1.0
O A:ASP4 3.6 47.8 1.0
CG A:HIS8 4.0 33.8 1.0
ND1 A:HIS8 4.0 33.7 1.0
C A:ASP4 4.1 47.0 1.0
O A:ALA3 4.3 47.1 1.0
CA A:ASP4 4.4 47.8 1.0
C A:LEU6 4.6 34.8 1.0
O A:HOH2106 4.7 51.9 1.0
CG2 A:VAL32 4.7 27.8 1.0
N A:LEU6 4.8 38.9 1.0

Zinc binding site 3 out of 4 in 2xxg

Go back to Zinc Binding Sites List in 2xxg
Zinc binding site 3 out of 4 in the Structure of the N90S Mutant of Nitrite Reductase From Alcaligenes Xylosoxidans


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 3 of Structure of the N90S Mutant of Nitrite Reductase From Alcaligenes Xylosoxidans within 5.0Å range:
probe atom residue distance (Å) B Occ
C:Zn1340

b:26.5
occ:1.00
O C:HOH2306 2.0 25.3 1.0
NE2 C:HIS165 2.0 20.0 1.0
OD2 C:ASP167 2.0 20.8 1.0
OD1 C:ASP167 2.4 22.5 1.0
CG C:ASP167 2.5 23.3 1.0
CE1 C:HIS165 2.9 21.0 1.0
CD2 C:HIS165 3.1 18.0 1.0
O C:HOH2245 3.9 40.8 1.0
OG1 C:THR234 3.9 20.0 1.0
CB C:ASP167 4.0 22.7 1.0
ND1 C:HIS165 4.1 20.2 1.0
CG C:HIS165 4.2 18.3 1.0
CB C:THR234 4.3 21.5 1.0
N C:THR234 4.4 21.7 1.0
N C:ASP167 4.6 21.9 1.0
O C:GLY232 4.7 26.1 1.0
CA C:ASP167 4.8 23.3 1.0

Zinc binding site 4 out of 4 in 2xxg

Go back to Zinc Binding Sites List in 2xxg
Zinc binding site 4 out of 4 in the Structure of the N90S Mutant of Nitrite Reductase From Alcaligenes Xylosoxidans


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 4 of Structure of the N90S Mutant of Nitrite Reductase From Alcaligenes Xylosoxidans within 5.0Å range:
probe atom residue distance (Å) B Occ
C:Zn1342

b:54.3
occ:0.50
O C:HOH2307 2.0 51.3 1.0
NE2 C:HIS8 2.5 38.8 1.0
O C:ASP4 3.4 50.9 1.0
CE1 C:HIS8 3.4 37.8 1.0
CD2 C:HIS8 3.4 38.3 1.0
C C:ASP4 3.8 50.0 1.0
CA C:ASP4 3.9 51.1 1.0
O C:ALA3 4.2 52.3 1.0
O C:HOH2095 4.3 48.2 1.0
O C:LEU6 4.3 38.3 1.0
ND1 C:HIS8 4.4 35.7 1.0
CG C:HIS8 4.5 37.4 1.0
CB C:ASP4 4.8 51.1 1.0
N C:LYS5 4.8 48.5 1.0
OD1 C:ASP4 4.8 51.4 1.0
N C:ASP4 4.9 51.7 1.0
C C:ALA3 4.9 52.3 1.0

Reference:

M.A.Hough, R.R.Eady, S.S.Hasnain. Identification of the Proton Channel to the Active Site Type 2 Cu Centre of Nitrite Reductase: Structural and Enzymatic Properties of the HIS254PHE and ASN90SER Mutants Biochemistry V. 47 13547 2008.
ISSN: ISSN 0006-2960
PubMed: 19053252
DOI: 10.1021/BI801369Y
Page generated: Wed Dec 16 04:00:47 2020

Last articles

Zn in 7RRP
Zn in 7OKY
Zn in 7OL0
Zn in 7OKX
Zn in 7ONB
Zn in 7RSF
Zn in 7OUF
Zn in 7OUG
Zn in 7OUH
Zn in 7RGN
© Copyright 2008-2020 by atomistry.com
Home   |    Site Map   |    Copyright   |    Contact us   |    Privacy