Zinc in PDB 2woo: Nucleotide-Free Form of S. Pombe GET3

All present enzymatic activity of Nucleotide-Free Form of S. Pombe GET3:
3.6.3.16;

The structure of Nucleotide-Free Form of S. Pombe GET3, PDB code: 2woo was solved by A.Mateja, A.Szlachcic, M.E.Downing, M.Dobosz, M.Mariappan, R.S.Hegde, R.J.Keenan, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	40.687 / 3.01
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	73.795, 92.922, 286.468, 90.00, 90.00, 90.00
R / Rfree (%)	23.73 / 28.8

The binding sites of Zinc atom in the Nucleotide-Free Form of S. Pombe GET3 (pdb code 2woo). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 3 binding sites of Zinc where determined in the Nucleotide-Free Form of S. Pombe GET3, PDB code: 2woo:
Jump to Zinc binding site number: 1; 2; 3;

Zinc binding site 1 out of 3 in the Nucleotide-Free Form of S. Pombe GET3


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Nucleotide-Free Form of S. Pombe GET3 within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn1323 b:0.8 occ:1.00 SG B:CYS268 2.3 96.7 1.0 SG A:CYS271 2.3 0.7 1.0 SG A:CYS268 2.3 0.2 1.0 SG B:CYS271 2.3 99.9 1.0 CB A:CYS268 3.2 0.1 1.0 CB B:CYS268 3.2 0.7 1.0 CB B:CYS271 3.3 99.0 1.0 CB A:CYS271 3.3 0.3 1.0 N B:CYS271 3.6 0.5 1.0 N A:CYS271 3.7 0.9 1.0 CA B:CYS271 3.9 0.5 1.0 CA A:CYS271 4.0 0.3 1.0 CB B:GLN270 4.3 0.5 1.0 CB A:GLN270 4.5 0.1 1.0 C B:GLN270 4.6 0.1 1.0 CA B:CYS268 4.6 0.1 1.0 CA A:CYS268 4.6 0.4 1.0 CG B:GLN270 4.7 0.3 1.0 C A:GLN270 4.8 0.3 1.0 CG A:GLN270 4.8 0.1 1.0 CA B:GLN270 4.9 0.6 1.0 C B:CYS268 5.0 0.3 1.0

Zinc binding site 2 out of 3 in the Nucleotide-Free Form of S. Pombe GET3


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Nucleotide-Free Form of S. Pombe GET3 within 5.0Å range: probe atom residue distance (Å) B Occ C:Zn1323 b:0.9 occ:1.00 SG D:CYS271 2.3 96.2 1.0 SG C:CYS271 2.3 93.3 1.0 SG D:CYS268 2.3 81.0 1.0 SG C:CYS268 2.3 0.6 1.0 CB C:CYS268 3.3 0.0 1.0 CB D:CYS268 3.4 0.8 1.0 CB C:CYS271 3.6 0.6 1.0 CB D:CYS271 3.6 0.4 1.0 N D:CYS271 4.1 0.2 1.0 N C:CYS271 4.2 0.9 1.0 CA D:CYS271 4.4 97.5 1.0 CA C:CYS271 4.4 0.1 1.0 CA C:CYS268 4.8 0.3 1.0 CB D:GLN270 4.8 97.8 1.0 CA D:CYS268 4.9 0.9 1.0 CB C:GLN270 4.9 0.2 1.0

Zinc binding site 3 out of 3 in the Nucleotide-Free Form of S. Pombe GET3


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 3 of Nucleotide-Free Form of S. Pombe GET3 within 5.0Å range: probe atom residue distance (Å) B Occ F:Zn1323 b:0.2 occ:1.00 SG F:CYS268 2.3 0.5 1.0 SG F:CYS271 2.3 0.8 1.0 SG E:CYS271 2.3 0.9 1.0 SG E:CYS268 2.4 1.0 1.0 CB E:CYS268 3.3 0.7 1.0 CB F:CYS271 3.3 0.3 1.0 N F:CYS271 3.4 0.8 1.0 CB F:CYS268 3.4 0.7 1.0 CB E:CYS271 3.5 0.8 1.0 CA F:CYS271 3.8 0.6 1.0 CB F:GLN270 4.0 0.5 1.0 N E:CYS271 4.0 0.9 1.0 CA E:CYS271 4.2 0.4 1.0 C F:GLN270 4.4 0.7 1.0 CG F:GLN270 4.4 0.6 1.0 CA F:GLN270 4.7 0.4 1.0 CB E:GLN270 4.7 0.6 1.0 CA E:CYS268 4.8 0.3 1.0 CA F:CYS268 4.8 0.4 1.0 N F:GLN270 4.9 0.3 1.0

A.Mateja, A.Szlachcic, M.E.Downing, M.Dobosz, M.Mariappan, R.S.Hegde, R.J.Keenan. The Structural Basis of Tail-Anchored Membrane Protein Recognition By GET3. Nature V. 461 361 2009.
ISSN: ISSN 0028-0836
PubMed: 19675567
DOI: 10.1038/NATURE08319