Atomistry » Zinc » PDB 2vqx-2w13 » 2vr8
Atomistry »
  Zinc »
    PDB 2vqx-2w13 »
      2vr8 »

Zinc in PDB 2vr8: Crystal Structure of G85R Als Mutant of Human Cu,Zn Superoxide Dismutase (Cuznsod) at 1.36 A Resolution

Enzymatic activity of Crystal Structure of G85R Als Mutant of Human Cu,Zn Superoxide Dismutase (Cuznsod) at 1.36 A Resolution

All present enzymatic activity of Crystal Structure of G85R Als Mutant of Human Cu,Zn Superoxide Dismutase (Cuznsod) at 1.36 A Resolution:
1.15.1.1;

Protein crystallography data

The structure of Crystal Structure of G85R Als Mutant of Human Cu,Zn Superoxide Dismutase (Cuznsod) at 1.36 A Resolution, PDB code: 2vr8 was solved by S.Antonyuk, X.Cao, S.V.Seetharaman, L.J.Whitson, A.B.Taylor, S.P.Holloway, R.W.Strange, P.A.Doucette, A.Tiwari, L.J.Hayward, S.Padua, J.A.Cohlberg, J.Selverstone Valentine, S.S.Hasnain, P.J.Hart, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 30.00 / 1.36
Space group P 1 21 1
Cell size a, b, c (Å), α, β, γ (°) 34.210, 56.647, 74.714, 90.00, 102.14, 90.00
R / Rfree (%) 11.3 / 15.3

Other elements in 2vr8:

The structure of Crystal Structure of G85R Als Mutant of Human Cu,Zn Superoxide Dismutase (Cuznsod) at 1.36 A Resolution also contains other interesting chemical elements:

Copper (Cu) 2 atoms

Zinc Binding Sites:

The binding sites of Zinc atom in the Crystal Structure of G85R Als Mutant of Human Cu,Zn Superoxide Dismutase (Cuznsod) at 1.36 A Resolution (pdb code 2vr8). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 6 binding sites of Zinc where determined in the Crystal Structure of G85R Als Mutant of Human Cu,Zn Superoxide Dismutase (Cuznsod) at 1.36 A Resolution, PDB code: 2vr8:
Jump to Zinc binding site number: 1; 2; 3; 4; 5; 6;

Zinc binding site 1 out of 6 in 2vr8

Go back to Zinc Binding Sites List in 2vr8
Zinc binding site 1 out of 6 in the Crystal Structure of G85R Als Mutant of Human Cu,Zn Superoxide Dismutase (Cuznsod) at 1.36 A Resolution


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Crystal Structure of G85R Als Mutant of Human Cu,Zn Superoxide Dismutase (Cuznsod) at 1.36 A Resolution within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Zn1157

b:11.8
occ:0.35
 CU A:CU1156 1.1 10.2 0.2
NE2 A:HIS120 1.9 11.4 1.0
O1 A:SO41159 2.1 9.9 0.5
NE2 A:HIS48 2.1 11.1 1.0
O A:HOH2265 2.2 25.6 0.5
ND1 A:HIS46 2.3 15.8 1.0
CE1 A:HIS120 2.6 10.8 1.0
CE1 A:HIS48 2.7 10.7 1.0
CD2 A:HIS120 3.1 10.3 1.0
CE1 A:HIS46 3.1 12.1 1.0
NE2 A:HIS63 3.2 12.5 1.0
S A:SO41159 3.3 9.9 0.5
CD2 A:HIS48 3.3 10.8 1.0
CG A:HIS46 3.4 11.1 1.0
O2 A:SO41159 3.6 10.8 0.5
CD2 A:HIS63 3.6 12.5 1.0
CB A:HIS46 3.8 11.4 1.0
ND1 A:HIS120 3.8 9.9 1.0
ND1 A:HIS48 4.0 9.9 1.0
CG A:HIS120 4.0 8.7 1.0
O4 A:SO41159 4.2 11.7 0.5
O3 A:SO41159 4.2 11.8 0.5
CG A:HIS48 4.3 9.4 1.0
CE1 A:HIS63 4.3 10.1 1.0
O A:HOH2269 4.3 12.1 0.3
NE2 A:HIS46 4.3 13.1 1.0
O A:HOH2241 4.3 20.1 0.7
CG1 A:VAL118 4.4 8.4 1.0
CD2 A:HIS46 4.5 12.4 1.0
O A:HOH2268 4.5 12.4 0.3
CB A:VAL118 4.5 8.3 1.0
NE A:ARG143 4.8 9.8 1.0
CG A:HIS63 4.8 11.7 1.0

Zinc binding site 2 out of 6 in 2vr8

Go back to Zinc Binding Sites List in 2vr8
Zinc binding site 2 out of 6 in the Crystal Structure of G85R Als Mutant of Human Cu,Zn Superoxide Dismutase (Cuznsod) at 1.36 A Resolution


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Crystal Structure of G85R Als Mutant of Human Cu,Zn Superoxide Dismutase (Cuznsod) at 1.36 A Resolution within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Zn1158

b:9.5
occ:0.60
 OD1 A:ASP83 2.0 9.0 1.0
ND1 A:HIS63 2.0 9.6 1.0
ND1 A:HIS71 2.0 10.1 1.0
ND1 A:HIS80 2.1 8.3 1.0
CG A:ASP83 2.8 8.7 1.0
OD2 A:ASP83 2.9 9.5 1.0
CE1 A:HIS71 2.9 8.3 1.0
CE1 A:HIS80 2.9 8.9 1.0
CE1 A:HIS63 2.9 10.1 1.0
CG A:HIS63 3.1 11.7 1.0
CG A:HIS80 3.1 8.3 1.0
CG A:HIS71 3.2 10.0 1.0
CB A:HIS63 3.5 10.4 1.0
CB A:HIS80 3.5 7.8 1.0
CB A:HIS71 3.7 9.1 1.0
CA A:HIS71 4.0 9.5 1.0
NE2 A:HIS80 4.0 10.0 1.0
NE2 A:HIS71 4.1 10.0 1.0
NE2 A:HIS63 4.1 12.5 1.0
O A:LYS136 4.1 12.8 1.0
CD2 A:HIS80 4.1 10.1 1.0
CD2 A:HIS63 4.2 12.5 1.0
CB A:ASP83 4.2 8.4 1.0
CD2 A:HIS71 4.2 9.8 1.0
CA A:ASP83 4.7 9.6 1.0
N A:HIS80 4.7 9.2 1.0
CA A:HIS80 4.8 8.6 1.0
O A:HOH2239 4.8 20.4 1.0
N A:GLY72 4.8 9.6 1.0
N A:ASP83 4.9 8.9 1.0
C A:HIS71 4.9 10.1 1.0
N A:HIS71 5.0 9.3 1.0
CA A:HIS63 5.0 10.3 1.0

Zinc binding site 3 out of 6 in 2vr8

Go back to Zinc Binding Sites List in 2vr8
Zinc binding site 3 out of 6 in the Crystal Structure of G85R Als Mutant of Human Cu,Zn Superoxide Dismutase (Cuznsod) at 1.36 A Resolution


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 3 of Crystal Structure of G85R Als Mutant of Human Cu,Zn Superoxide Dismutase (Cuznsod) at 1.36 A Resolution within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Zn1160

b:12.8
occ:0.70
 N A:SCN1162 1.9 12.2 1.0
N A:SCN1161 1.9 10.8 1.0
OE1 A:GLU24 2.0 10.4 1.0
CD A:GLU24 2.7 10.9 1.0
OE2 A:GLU24 2.7 12.2 1.0
C A:SCN1162 3.0 12.2 1.0
C A:SCN1161 3.0 10.9 1.0
CG A:GLU24 4.1 11.1 1.0
O A:HOH2062 4.1 18.6 1.0
S A:SCN1161 4.8 11.0 1.0
S A:SCN1162 4.8 16.6 1.0
O A:HOH2060 5.0 12.6 0.5

Zinc binding site 4 out of 6 in 2vr8

Go back to Zinc Binding Sites List in 2vr8
Zinc binding site 4 out of 6 in the Crystal Structure of G85R Als Mutant of Human Cu,Zn Superoxide Dismutase (Cuznsod) at 1.36 A Resolution


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 4 of Crystal Structure of G85R Als Mutant of Human Cu,Zn Superoxide Dismutase (Cuznsod) at 1.36 A Resolution within 5.0Å range:
probe atom residue distance (Å) B Occ
F:Zn1157

b:11.1
occ:0.35
 CU F:CU1156 1.1 12.6 0.2
NE2 F:HIS120 1.9 11.3 1.0
O1 F:SO41159 2.0 9.5 0.7
NE2 F:HIS48 2.1 10.8 1.0
ND1 F:HIS46 2.3 14.3 1.0
CE1 F:HIS120 2.7 11.6 1.0
CE1 F:HIS48 2.7 10.1 1.0
CD2 F:HIS120 3.1 11.8 1.0
CE1 F:HIS46 3.1 14.1 1.0
S F:SO41159 3.2 11.9 0.7
NE2 F:HIS63 3.3 13.3 1.0
CD2 F:HIS48 3.3 10.8 1.0
CG F:HIS46 3.4 11.4 1.0
O2 F:SO41159 3.5 14.0 0.7
CD2 F:HIS63 3.7 12.3 1.0
CB F:HIS46 3.7 11.9 1.0
ND1 F:HIS120 3.9 11.7 1.0
ND1 F:HIS48 4.0 9.4 1.0
O4 F:SO41159 4.1 14.7 0.7
CG F:HIS120 4.1 9.9 1.0
O3 F:SO41159 4.2 11.8 0.7
O F:HOH2250 4.2 9.5 0.3
CG F:HIS48 4.3 8.9 1.0
NE2 F:HIS46 4.3 12.5 1.0
CE1 F:HIS63 4.3 9.9 1.0
O F:HOH2224 4.4 11.3 0.5
CG1 F:VAL118 4.4 9.4 1.0
CD2 F:HIS46 4.4 13.4 1.0
CB F:VAL118 4.5 7.7 1.0
NE F:ARG143 4.7 10.2 1.0
CG F:HIS63 4.9 11.2 1.0

Zinc binding site 5 out of 6 in 2vr8

Go back to Zinc Binding Sites List in 2vr8
Zinc binding site 5 out of 6 in the Crystal Structure of G85R Als Mutant of Human Cu,Zn Superoxide Dismutase (Cuznsod) at 1.36 A Resolution


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 5 of Crystal Structure of G85R Als Mutant of Human Cu,Zn Superoxide Dismutase (Cuznsod) at 1.36 A Resolution within 5.0Å range:
probe atom residue distance (Å) B Occ
F:Zn1158

b:10.9
occ:0.65
 OD1 F:ASP83 2.0 9.5 1.0
ND1 F:HIS63 2.0 10.4 1.0
ND1 F:HIS80 2.0 9.2 1.0
ND1 F:HIS71 2.1 10.0 1.0
CG F:ASP83 2.8 9.9 1.0
OD2 F:ASP83 2.9 10.7 1.0
CE1 F:HIS71 2.9 10.0 1.0
CE1 F:HIS80 2.9 9.7 1.0
CE1 F:HIS63 2.9 9.9 1.0
CG F:HIS63 3.1 11.2 1.0
CG F:HIS80 3.1 9.9 1.0
CG F:HIS71 3.2 9.7 1.0
CB F:HIS63 3.4 11.1 1.0
CB F:HIS80 3.5 9.4 1.0
CB F:HIS71 3.7 9.3 1.0
CA F:HIS71 4.0 10.4 1.0
NE2 F:HIS80 4.1 10.7 1.0
NE2 F:HIS71 4.1 10.2 1.0
NE2 F:HIS63 4.1 13.3 1.0
O F:LYS136 4.1 12.6 1.0
CD2 F:HIS80 4.1 10.2 1.0
CD2 F:HIS63 4.2 12.3 1.0
CB F:ASP83 4.2 9.6 1.0
CD2 F:HIS71 4.2 10.5 1.0
CA F:ASP83 4.7 9.6 1.0
N F:HIS80 4.7 9.5 1.0
CA F:HIS80 4.8 8.5 1.0
O F:HOH2222 4.8 19.1 1.0
N F:ASP83 4.8 9.5 1.0
N F:GLY72 4.9 11.6 1.0
N F:HIS71 5.0 11.1 1.0
CA F:HIS63 5.0 10.3 1.0
C F:HIS71 5.0 11.2 1.0

Zinc binding site 6 out of 6 in 2vr8

Go back to Zinc Binding Sites List in 2vr8
Zinc binding site 6 out of 6 in the Crystal Structure of G85R Als Mutant of Human Cu,Zn Superoxide Dismutase (Cuznsod) at 1.36 A Resolution


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 6 of Crystal Structure of G85R Als Mutant of Human Cu,Zn Superoxide Dismutase (Cuznsod) at 1.36 A Resolution within 5.0Å range:
probe atom residue distance (Å) B Occ
F:Zn1160

b:13.3
occ:0.70
 N F:SCN1162 1.9 13.0 1.0
N F:SCN1161 1.9 14.5 1.0
OE1 F:GLU24 1.9 13.8 1.0
CD F:GLU24 2.7 13.0 1.0
OE2 F:GLU24 2.8 16.0 1.0
C F:SCN1162 3.0 10.7 1.0
C F:SCN1161 3.0 14.2 1.0
CG F:GLU24 4.1 12.5 1.0
O F:HOH2048 4.2 18.7 0.5
S F:SCN1162 4.7 10.6 1.0
S F:SCN1161 4.8 19.5 1.0
O F:HOH2049 4.8 17.6 1.0

Reference:

X.Cao, S.Antonyuk, S.V.Seetharaman, L.J.Whitson, A.B.Taylor, S.P.Holloway, R.W.Strange, P.A.Doucette, A.Tiwari, L.J.Hayward, S.Padua, J.A.Cohlberg, J.Selverstone Valentine, S.S.Hasnain, P.J.Hart. Structures of the G85R Variant of SOD1 in Familial Amyotrophic Lateral Sclerosis. J.Biol.Chem. V. 283 16169 2008.
ISSN: ISSN 0021-9258
PubMed: 18378676
DOI: 10.1074/JBC.M801522200
Page generated: Thu Oct 17 04:31:21 2024

Last articles

Zn in 9JYW
Zn in 9IR4
Zn in 9IR3
Zn in 9GMX
Zn in 9GMW
Zn in 9JEJ
Zn in 9ERF
Zn in 9ERE
Zn in 9EGV
Zn in 9EGW
© Copyright 2008-2020 by atomistry.com
Home   |    Site Map   |    Copyright   |    Contact us   |    Privacy