Atomistry » Zinc » PDB 2q1z-2ql1 » 2qin
Atomistry »
  Zinc »
    PDB 2q1z-2ql1 »
      2qin »

Zinc in PDB 2qin: Stenotrophomonas Maltophilia L1 Metallo-Beta-Lactamase Asp-120 Cys Mutant

Enzymatic activity of Stenotrophomonas Maltophilia L1 Metallo-Beta-Lactamase Asp-120 Cys Mutant

All present enzymatic activity of Stenotrophomonas Maltophilia L1 Metallo-Beta-Lactamase Asp-120 Cys Mutant:
3.5.2.6;

Protein crystallography data

The structure of Stenotrophomonas Maltophilia L1 Metallo-Beta-Lactamase Asp-120 Cys Mutant, PDB code: 2qin was solved by J.Spencer, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 26.84 / 1.76
Space group P 1 21 1
Cell size a, b, c (Å), α, β, γ (°) 66.141, 112.845, 78.352, 90.00, 113.35, 90.00
R / Rfree (%) 17.3 / 21.2

Other elements in 2qin:

The structure of Stenotrophomonas Maltophilia L1 Metallo-Beta-Lactamase Asp-120 Cys Mutant also contains other interesting chemical elements:

Magnesium (Mg) 2 atoms

Zinc Binding Sites:

The binding sites of Zinc atom in the Stenotrophomonas Maltophilia L1 Metallo-Beta-Lactamase Asp-120 Cys Mutant (pdb code 2qin). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 9 binding sites of Zinc where determined in the Stenotrophomonas Maltophilia L1 Metallo-Beta-Lactamase Asp-120 Cys Mutant, PDB code: 2qin:
Jump to Zinc binding site number: 1; 2; 3; 4; 5; 6; 7; 8; 9;

Zinc binding site 1 out of 9 in 2qin

Go back to Zinc Binding Sites List in 2qin
Zinc binding site 1 out of 9 in the Stenotrophomonas Maltophilia L1 Metallo-Beta-Lactamase Asp-120 Cys Mutant


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Stenotrophomonas Maltophilia L1 Metallo-Beta-Lactamase Asp-120 Cys Mutant within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Zn2001

b:21.8
occ:1.00
 O A:HOH2192 1.8 19.0 1.0
NE2 A:HIS121 2.0 15.8 1.0
NE2 A:HIS263 2.1 16.7 1.0
SG A:CYS120 2.4 15.8 1.0
CD2 A:HIS121 2.9 15.7 1.0
CE1 A:HIS263 3.0 16.9 1.0
CE1 A:HIS121 3.0 15.8 1.0
O A:HOH2313 3.1 40.9 1.0
CD2 A:HIS263 3.1 16.5 1.0
CB A:CYS120 3.5 14.0 1.0
ZN A:ZN2002 3.5 17.6 1.0
CG A:HIS121 4.0 14.4 1.0
ND1 A:HIS121 4.0 15.0 1.0
NE2 A:HIS116 4.1 12.9 1.0
ND1 A:HIS263 4.1 16.9 1.0
CG A:HIS263 4.2 16.8 1.0
O A:HOH2203 4.3 29.6 1.0
CE1 A:HIS116 4.3 13.8 1.0
CA A:CYS120 4.6 13.6 1.0
C A:CYS120 4.7 13.6 1.0
OH A:TYR32 4.8 23.3 1.0
CB A:HIS118 4.8 12.3 1.0
O A:CYS120 4.9 14.0 1.0
N A:CYS120 5.0 13.0 1.0

Zinc binding site 2 out of 9 in 2qin

Go back to Zinc Binding Sites List in 2qin
Zinc binding site 2 out of 9 in the Stenotrophomonas Maltophilia L1 Metallo-Beta-Lactamase Asp-120 Cys Mutant


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Stenotrophomonas Maltophilia L1 Metallo-Beta-Lactamase Asp-120 Cys Mutant within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Zn2002

b:17.6
occ:1.00
 NE2 A:HIS196 2.0 13.5 1.0
O A:HOH2192 2.1 19.0 1.0
ND1 A:HIS118 2.1 12.8 1.0
NE2 A:HIS116 2.1 12.9 1.0
CD2 A:HIS196 2.9 13.9 1.0
CE1 A:HIS118 3.0 12.7 1.0
O A:HOH2313 3.0 40.9 1.0
CD2 A:HIS116 3.0 13.0 1.0
CE1 A:HIS196 3.1 13.8 1.0
CG A:HIS118 3.1 12.2 1.0
CE1 A:HIS116 3.1 13.8 1.0
CB A:HIS118 3.5 12.3 1.0
ZN A:ZN2001 3.5 21.8 1.0
CG A:HIS196 4.1 13.6 1.0
O A:HOH2203 4.1 29.6 1.0
NE2 A:HIS118 4.1 13.0 1.0
CG A:HIS116 4.2 12.0 1.0
ND1 A:HIS196 4.2 13.8 1.0
ND1 A:HIS116 4.2 13.7 1.0
CD2 A:HIS118 4.2 12.8 1.0
CD2 A:HIS121 4.3 15.7 1.0
NE2 A:HIS121 4.3 15.8 1.0
SG A:CYS120 4.6 15.8 1.0
OG A:SER221 4.8 17.9 1.0
CA A:HIS118 5.0 11.9 1.0

Zinc binding site 3 out of 9 in 2qin

Go back to Zinc Binding Sites List in 2qin
Zinc binding site 3 out of 9 in the Stenotrophomonas Maltophilia L1 Metallo-Beta-Lactamase Asp-120 Cys Mutant


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 3 of Stenotrophomonas Maltophilia L1 Metallo-Beta-Lactamase Asp-120 Cys Mutant within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Zn2001

b:16.5
occ:1.00
 O B:HOH4232 1.9 14.3 1.0
NE2 B:HIS121 2.0 11.8 1.0
NE2 B:HIS263 2.1 10.8 1.0
SG B:CYS120 2.5 13.1 1.0
CD2 B:HIS121 2.9 11.5 1.0
CE1 B:HIS121 3.0 11.8 1.0
CE1 B:HIS263 3.0 12.1 1.0
CD2 B:HIS263 3.1 11.2 1.0
O B:HOH4234 3.3 39.3 1.0
CB B:CYS120 3.6 10.7 1.0
ZN B:ZN2002 3.6 13.1 1.0
ND1 B:HIS121 4.0 10.8 1.0
CG B:HIS121 4.0 10.9 1.0
NE2 B:HIS116 4.1 11.3 1.0
O B:HOH4230 4.1 33.4 1.0
ND1 B:HIS263 4.2 10.9 1.0
CG B:HIS263 4.2 10.8 1.0
CE1 B:HIS116 4.3 10.8 1.0
O B:HOH4241 4.5 29.3 1.0
CA B:CYS120 4.6 10.6 1.0
C B:CYS120 4.8 10.5 1.0
CB B:HIS118 4.9 8.9 1.0
OH B:TYR32 4.9 18.1 1.0
O B:CYS120 5.0 10.5 1.0

Zinc binding site 4 out of 9 in 2qin

Go back to Zinc Binding Sites List in 2qin
Zinc binding site 4 out of 9 in the Stenotrophomonas Maltophilia L1 Metallo-Beta-Lactamase Asp-120 Cys Mutant


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 4 of Stenotrophomonas Maltophilia L1 Metallo-Beta-Lactamase Asp-120 Cys Mutant within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Zn2002

b:13.1
occ:1.00
 O B:HOH4232 1.9 14.3 1.0
NE2 B:HIS196 2.0 9.7 1.0
ND1 B:HIS118 2.1 9.6 1.0
NE2 B:HIS116 2.1 11.3 1.0
O B:HOH4241 2.9 29.3 1.0
CD2 B:HIS196 2.9 9.4 1.0
CE1 B:HIS118 3.0 10.2 1.0
CE1 B:HIS116 3.0 10.8 1.0
CD2 B:HIS116 3.1 9.9 1.0
CE1 B:HIS196 3.1 10.2 1.0
CG B:HIS118 3.1 9.0 1.0
O B:HOH4234 3.4 39.3 1.0
CB B:HIS118 3.5 8.9 1.0
ZN B:ZN2001 3.6 16.5 1.0
O B:HOH4230 4.0 33.4 1.0
ND1 B:HIS116 4.1 11.4 1.0
CG B:HIS196 4.1 9.4 1.0
NE2 B:HIS118 4.1 9.0 1.0
CG B:HIS116 4.1 10.1 1.0
ND1 B:HIS196 4.2 9.3 1.0
CD2 B:HIS118 4.2 9.7 1.0
CD2 B:HIS121 4.3 11.5 1.0
NE2 B:HIS121 4.3 11.8 1.0
SG B:CYS120 4.7 13.1 1.0
OG B:SER221 4.9 8.8 0.5
CA B:HIS118 4.9 9.2 1.0

Zinc binding site 5 out of 9 in 2qin

Go back to Zinc Binding Sites List in 2qin
Zinc binding site 5 out of 9 in the Stenotrophomonas Maltophilia L1 Metallo-Beta-Lactamase Asp-120 Cys Mutant


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 5 of Stenotrophomonas Maltophilia L1 Metallo-Beta-Lactamase Asp-120 Cys Mutant within 5.0Å range:
probe atom residue distance (Å) B Occ
C:Zn2001

b:24.8
occ:1.00
 O C:HOH2241 1.9 20.0 1.0
NE2 C:HIS121 2.0 18.7 1.0
NE2 C:HIS263 2.3 20.8 1.0
SG C:CYS120 2.4 18.8 1.0
CD2 C:HIS121 2.8 17.5 1.0
CE1 C:HIS121 2.9 18.5 1.0
CE1 C:HIS263 3.1 21.8 1.0
O C:HOH2252 3.2 40.9 1.0
CD2 C:HIS263 3.3 21.7 1.0
CB C:CYS120 3.5 17.5 1.0
ZN C:ZN2002 3.6 20.3 1.0
CG C:HIS121 3.9 18.0 1.0
ND1 C:HIS121 3.9 18.6 1.0
NE2 C:HIS116 4.1 16.6 1.0
ND1 C:HIS263 4.2 20.9 1.0
CE1 C:HIS116 4.3 16.4 1.0
CG C:HIS263 4.4 22.0 1.0
CA C:CYS120 4.5 17.1 1.0
C C:CYS120 4.6 17.2 1.0
O C:CYS120 4.8 17.4 1.0
O C:HOH2251 4.8 41.3 1.0
OH C:TYR32 4.8 23.3 1.0
CB C:HIS118 4.9 15.3 1.0
N C:CYS120 4.9 16.7 1.0

Zinc binding site 6 out of 9 in 2qin

Go back to Zinc Binding Sites List in 2qin
Zinc binding site 6 out of 9 in the Stenotrophomonas Maltophilia L1 Metallo-Beta-Lactamase Asp-120 Cys Mutant


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 6 of Stenotrophomonas Maltophilia L1 Metallo-Beta-Lactamase Asp-120 Cys Mutant within 5.0Å range:
probe atom residue distance (Å) B Occ
C:Zn2002

b:20.3
occ:1.00
 NE2 C:HIS196 2.0 17.0 1.0
O C:HOH2241 2.1 20.0 1.0
NE2 C:HIS116 2.1 16.6 1.0
ND1 C:HIS118 2.2 16.1 1.0
CD2 C:HIS196 3.0 16.3 1.0
O C:HOH2252 3.0 40.9 1.0
CD2 C:HIS116 3.0 16.2 1.0
CE1 C:HIS116 3.1 16.4 1.0
CE1 C:HIS196 3.1 17.1 1.0
CE1 C:HIS118 3.1 16.2 1.0
CG C:HIS118 3.2 15.9 1.0
O C:HOH2251 3.4 41.3 1.0
CB C:HIS118 3.5 15.3 1.0
ZN C:ZN2001 3.6 24.8 1.0
ND1 C:HIS116 4.1 17.0 1.0
CG C:HIS116 4.1 15.8 1.0
CG C:HIS196 4.1 15.8 1.0
ND1 C:HIS196 4.1 16.7 1.0
NE2 C:HIS118 4.2 16.4 1.0
CD2 C:HIS121 4.3 17.5 1.0
NE2 C:HIS121 4.3 18.7 1.0
CD2 C:HIS118 4.3 15.2 1.0
SG C:CYS120 4.7 18.8 1.0
OG C:SER221 5.0 22.1 1.0
CA C:HIS118 5.0 15.2 1.0
O C:HOH2200 5.0 44.3 1.0

Zinc binding site 7 out of 9 in 2qin

Go back to Zinc Binding Sites List in 2qin
Zinc binding site 7 out of 9 in the Stenotrophomonas Maltophilia L1 Metallo-Beta-Lactamase Asp-120 Cys Mutant


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 7 of Stenotrophomonas Maltophilia L1 Metallo-Beta-Lactamase Asp-120 Cys Mutant within 5.0Å range:
probe atom residue distance (Å) B Occ
D:Zn2001

b:18.1
occ:1.00
 O D:HOH4261 2.0 13.3 1.0
NE2 D:HIS121 2.0 13.1 1.0
NE2 D:HIS263 2.1 11.1 1.0
SG D:CYS120 2.4 14.5 1.0
CE1 D:HIS121 3.0 12.5 1.0
CD2 D:HIS121 3.0 11.8 1.0
CE1 D:HIS263 3.0 11.5 1.0
CD2 D:HIS263 3.2 11.8 1.0
O D:HOH4378 3.2 41.0 1.0
ZN D:ZN2002 3.6 13.4 1.0
CB D:CYS120 3.7 12.1 1.0
ND1 D:HIS121 4.0 11.7 1.0
CG D:HIS121 4.0 12.0 1.0
NE2 D:HIS116 4.1 9.9 1.0
ND1 D:HIS263 4.1 11.6 1.0
O D:HOH4207 4.2 32.0 1.0
CG D:HIS263 4.2 10.6 1.0
CE1 D:HIS116 4.3 10.4 1.0
O D:HOH4347 4.5 29.9 1.0
CA D:CYS120 4.7 11.8 1.0
C D:CYS120 4.8 11.6 1.0
O D:CYS120 4.9 11.9 1.0
CB D:HIS118 4.9 10.2 1.0
OH D:TYR32 4.9 23.1 1.0
NE2 D:HIS196 5.0 9.9 1.0

Zinc binding site 8 out of 9 in 2qin

Go back to Zinc Binding Sites List in 2qin
Zinc binding site 8 out of 9 in the Stenotrophomonas Maltophilia L1 Metallo-Beta-Lactamase Asp-120 Cys Mutant


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 8 of Stenotrophomonas Maltophilia L1 Metallo-Beta-Lactamase Asp-120 Cys Mutant within 5.0Å range:
probe atom residue distance (Å) B Occ
D:Zn2002

b:13.4
occ:1.00
 O D:HOH4261 1.9 13.3 1.0
NE2 D:HIS196 2.0 9.9 1.0
ND1 D:HIS118 2.0 10.6 1.0
NE2 D:HIS116 2.1 9.9 1.0
O D:HOH4347 2.8 29.9 1.0
CD2 D:HIS196 2.9 8.4 1.0
CE1 D:HIS118 2.9 11.1 1.0
CD2 D:HIS116 3.0 9.6 1.0
CE1 D:HIS116 3.0 10.4 1.0
CG D:HIS118 3.1 10.7 1.0
CE1 D:HIS196 3.1 10.4 1.0
O D:HOH4378 3.3 41.0 1.0
CB D:HIS118 3.5 10.2 1.0
ZN D:ZN2001 3.6 18.1 1.0
NE2 D:HIS118 4.1 10.8 1.0
CG D:HIS196 4.1 9.3 1.0
ND1 D:HIS116 4.1 11.1 1.0
CG D:HIS116 4.1 9.9 1.0
ND1 D:HIS196 4.2 9.3 1.0
CD2 D:HIS118 4.2 11.4 1.0
O D:HOH4207 4.2 32.0 1.0
NE2 D:HIS121 4.3 13.1 1.0
CD2 D:HIS121 4.3 11.8 1.0
SG D:CYS120 4.6 14.5 1.0
CA D:HIS118 5.0 10.3 1.0
OG D:SER221 5.0 12.6 1.0

Zinc binding site 9 out of 9 in 2qin

Go back to Zinc Binding Sites List in 2qin
Zinc binding site 9 out of 9 in the Stenotrophomonas Maltophilia L1 Metallo-Beta-Lactamase Asp-120 Cys Mutant


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 9 of Stenotrophomonas Maltophilia L1 Metallo-Beta-Lactamase Asp-120 Cys Mutant within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Zn3001

b:10.7
occ:0.50
 O B:HOH4240 2.2 7.0 1.0
NE2 B:HIS51 2.2 13.3 1.0
CD2 B:HIS51 3.0 12.8 1.0
CE1 B:HIS51 3.3 14.0 1.0
CD B:PRO76 3.9 16.8 1.0
CG B:HIS51 4.3 12.8 1.0
ND1 B:HIS51 4.4 13.8 1.0
CG B:PRO76 4.5 17.3 1.0
CA B:THR75 4.8 15.8 1.0
O B:GLN74 4.9 14.8 1.0
CB B:THR75 5.0 15.9 1.0

Reference:

J.Crisp, R.Conners, J.D.Garrity, A.L.Carenbauer, M.W.Crowder, J.Spencer. Structural Basis For the Role of Asp-120 in Metallo-Beta-Lactamases. Biochemistry V. 46 10664 2007.
ISSN: ISSN 0006-2960
PubMed: 17715946
DOI: 10.1021/BI700707U
Page generated: Thu Oct 17 03:28:15 2024

Last articles

Zn in 9JYW
Zn in 9IR4
Zn in 9IR3
Zn in 9GMX
Zn in 9GMW
Zn in 9JEJ
Zn in 9ERF
Zn in 9ERE
Zn in 9EGV
Zn in 9EGW
© Copyright 2008-2020 by atomistry.com
Home   |    Site Map   |    Copyright   |    Contact us   |    Privacy