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Zinc in PDB 2owo: Last Stop on the Road to Repair: Structure of E.Coli Dna Ligase Bound to Nicked Dna-Adenylate

Enzymatic activity of Last Stop on the Road to Repair: Structure of E.Coli Dna Ligase Bound to Nicked Dna-Adenylate

All present enzymatic activity of Last Stop on the Road to Repair: Structure of E.Coli Dna Ligase Bound to Nicked Dna-Adenylate:
6.5.1.2;

Protein crystallography data

The structure of Last Stop on the Road to Repair: Structure of E.Coli Dna Ligase Bound to Nicked Dna-Adenylate, PDB code: 2owo was solved by S.Shuman, J.Nandakumar, P.A.Nair, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

*Resolution Low / High (Å)*	30.34 / 2.30
*Space group*	C 1 2 1
*Cell size a, b, c (Å), α, β, γ (°)*	110.441, 99.273, 86.245, 90.00, 105.33, 90.00
*R / R_free (%)*	21.7 / 25.4

Zinc Binding Sites:

The binding sites of Zinc atom in the Last Stop on the Road to Repair: Structure of E.Coli Dna Ligase Bound to Nicked Dna-Adenylate (pdb code 2owo). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total only one binding site of Zinc was determined in the Last Stop on the Road to Repair: Structure of E.Coli Dna Ligase Bound to Nicked Dna-Adenylate, PDB code: 2owo:

Zinc binding site 1 out of 1 in 2owo

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Zinc Binding Sites List in 2owo

Zinc binding site 1 out of 1 in the Last Stop on the Road to Repair: Structure of E.Coli Dna Ligase Bound to Nicked Dna-Adenylate

Mono view

Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Last Stop on the Road to Repair: Structure of E.Coli Dna Ligase Bound to Nicked Dna-Adenylate within 5.0Å range:

probe	atom	residue	distance (Å)	B	Occ
A:Zn672 b:42.3 occ:1.00	SG	A:CYS432	2.4	47.1	1.0
	SG	A:CYS426	2.5	46.0	1.0
	SG	A:CYS408	2.5	43.9	1.0
	SG	A:CYS411	2.5	53.1	1.0
	CB	A:CYS432	3.0	42.7	1.0
	CB	A:CYS408	3.2	47.3	1.0
	CB	A:CYS411	3.4	51.2	1.0
	CB	A:CYS426	3.6	42.1	1.0
	N	A:CYS411	3.7	50.4	1.0
	CA	A:CYS432	4.0	42.4	1.0
	CA	A:CYS411	4.1	50.9	1.0
	NE2	A:GLN435	4.3	38.0	1.0
	CB	A:VAL410	4.4	49.8	1.0
	CA	A:CYS408	4.7	48.7	1.0
	C	A:VAL410	4.8	50.2	1.0
	CG1	A:VAL331	4.8	22.9	1.0
	C	A:CYS411	4.8	50.6	1.0
	CA	A:VAL410	4.9	50.0	1.0
	N	A:VAL410	4.9	50.1	1.0
	N	A:GLY412	5.0	50.6	1.0
	C	A:CYS432	5.0	39.8	1.0
	CA	A:CYS426	5.0	43.2	1.0

Reference:

J.Nandakumar, P.A.Nair, S.Shuman. Last Stop on the Road to Repair: Structure of E. Coli Dna Ligase Bound to Nicked Dna-Adenylate. Mol.Cell V. 26 257 2007.
ISSN: ISSN 1097-2765
PubMed: 17466627
DOI: 10.1016/J.MOLCEL.2007.02.026

Page generated: Thu Oct 17 02:50:39 2024

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