Zinc in PDB 2hqk: Crystal Structure of A Monomeric Cyan Fluorescent Protein Derived From Clavularia

The structure of Crystal Structure of A Monomeric Cyan Fluorescent Protein Derived From Clavularia, PDB code: 2hqk was solved by J.N.Henderson, R.E.Campbell, H.Ai, S.J.Remington, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	10.00 / 1.19
Space group	C 1 2 1
Cell size a, b, c (Å), α, β, γ (°)	89.830, 38.020, 61.119, 90.00, 90.81, 90.00
R / Rfree (%)	14.9 / 20.6

The structure of Crystal Structure of A Monomeric Cyan Fluorescent Protein Derived From Clavularia also contains other interesting chemical elements:

Chlorine

(Cl)

1 atom

The binding sites of Zinc atom in the Crystal Structure of A Monomeric Cyan Fluorescent Protein Derived From Clavularia (pdb code 2hqk). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 3 binding sites of Zinc where determined in the Crystal Structure of A Monomeric Cyan Fluorescent Protein Derived From Clavularia, PDB code: 2hqk:
Jump to Zinc binding site number: 1; 2; 3;

Zinc binding site 1 out of 3 in the Crystal Structure of A Monomeric Cyan Fluorescent Protein Derived From Clavularia


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Crystal Structure of A Monomeric Cyan Fluorescent Protein Derived From Clavularia within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn401 b:18.8 occ:0.90 OD1 A:ASP116 1.9 19.2 1.0 CL A:CL404 2.4 24.1 1.0 CG A:ASP116 2.8 23.1 1.0 OD2 A:ASP116 3.1 32.4 1.0 O A:HOH585 4.1 34.3 0.9 CB A:ASP116 4.2 23.6 1.0 N A:ASP116 4.3 19.0 1.0 C A:ASP116 4.6 17.8 1.0 CA A:ASP116 4.6 19.5 1.0 O A:ASP116 4.7 21.6 1.0 CB A:GLU115 4.7 18.9 1.0 CB A:SER117 4.9 17.3 1.0 N A:SER117 4.9 17.2 1.0

Zinc binding site 2 out of 3 in the Crystal Structure of A Monomeric Cyan Fluorescent Protein Derived From Clavularia


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Crystal Structure of A Monomeric Cyan Fluorescent Protein Derived From Clavularia within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn402 b:27.4 occ:0.90 OD1 A:ASP77 1.9 26.5 1.0 O A:HOH559 2.1 36.6 1.0 CG A:ASP77 2.8 23.3 1.0 OD2 A:ASP77 3.0 27.7 1.0 CB A:ASP77 4.2 21.4 1.0 O A:HOH480 4.2 31.1 1.0 O A:HOH741 4.3 45.3 1.0

Zinc binding site 3 out of 3 in the Crystal Structure of A Monomeric Cyan Fluorescent Protein Derived From Clavularia


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 3 of Crystal Structure of A Monomeric Cyan Fluorescent Protein Derived From Clavularia within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn403 b:38.2 occ:0.65 OE1 A:GLU200 1.9 36.1 0.7 CD A:GLU200 2.3 28.3 0.7 O A:HOH534 2.3 29.9 1.0 OE2 A:GLU200 2.4 30.2 0.7 O A:HOH594 2.8 43.2 1.0 CG A:GLU200 3.7 24.4 0.7 O A:HOH635 4.0 44.4 1.0 CB A:GLU200 4.7 21.2 1.0 CD2 A:LEU202 4.9 26.5 1.0

H.W.Ai, J.N.Henderson, S.J.Remington, R.E.Campbell. Directed Evolution of A Monomeric, Bright and Photostable Version of Clavularia Cyan Fluorescent Protein: Structural Characterization and Applications in Fluorescence Imaging. Biochem.J. V. 400 531 2006.
ISSN: ISSN 0264-6021
PubMed: 16859491
DOI: 10.1042/BJ20060874