Zinc in PDB 2h1j: 3.1 A X-Ray Structure of Putative Oligoendopeptidase F: Crystals Grown By Microfluidic Seeding

The structure of 3.1 A X-Ray Structure of Putative Oligoendopeptidase F: Crystals Grown By Microfluidic Seeding, PDB code: 2h1j was solved by C.J.Gerdts, V.Tereshko, I.Dementieva, F.Collart, A.Joachimiak, A.Kossiakoff, R.F.Ismagilov, Accelerated Technologies Center For Geneto 3D Structure (Atcg3D), with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	20.00 / 3.10
Space group	P 31 2 1
Cell size a, b, c (Å), α, β, γ (°)	119.324, 119.324, 248.736, 90.00, 90.00, 120.00
R / Rfree (%)	18.7 / 22.5

The binding sites of Zinc atom in the 3.1 A X-Ray Structure of Putative Oligoendopeptidase F: Crystals Grown By Microfluidic Seeding (pdb code 2h1j). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the 3.1 A X-Ray Structure of Putative Oligoendopeptidase F: Crystals Grown By Microfluidic Seeding, PDB code: 2h1j:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the 3.1 A X-Ray Structure of Putative Oligoendopeptidase F: Crystals Grown By Microfluidic Seeding


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of 3.1 A X-Ray Structure of Putative Oligoendopeptidase F: Crystals Grown By Microfluidic Seeding within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn601 b:76.2 occ:1.00 OE2 A:GLU384 2.0 75.3 1.0 OE1 A:GLU384 2.0 76.0 1.0 NE2 A:HIS360 2.1 71.0 1.0 NE2 A:HIS356 2.1 71.9 1.0 CD A:GLU384 2.2 75.0 1.0 O A:HOH602 2.5 37.1 1.0 CE1 A:HIS356 2.9 70.2 1.0 CE1 A:HIS360 3.0 69.6 1.0 CD2 A:HIS360 3.1 70.0 1.0 CD2 A:HIS356 3.3 70.3 1.0 CE2 A:TYR490 3.5 69.0 1.0 OG A:SER387 3.7 75.1 1.0 OH A:TYR490 3.7 68.9 1.0 CG A:GLU384 3.7 73.2 1.0 ND1 A:HIS356 4.1 70.0 1.0 CZ A:TYR490 4.1 69.1 1.0 ND1 A:HIS360 4.1 69.7 1.0 CG A:HIS360 4.2 69.8 1.0 OH A:TYR486 4.3 71.1 1.0 CG A:HIS356 4.3 69.8 1.0 CB A:SER387 4.3 75.4 1.0 CD2 A:TYR490 4.6 68.7 1.0 CB A:GLU384 4.6 72.5 1.0 OE1 A:GLU357 4.9 72.2 1.0 CA A:GLU384 4.9 72.6 1.0 OE2 A:GLU357 5.0 71.6 1.0

Zinc binding site 2 out of 2 in the 3.1 A X-Ray Structure of Putative Oligoendopeptidase F: Crystals Grown By Microfluidic Seeding


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of 3.1 A X-Ray Structure of Putative Oligoendopeptidase F: Crystals Grown By Microfluidic Seeding within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn701 b:75.8 occ:1.00 OE2 B:GLU384 2.0 74.2 1.0 NE2 B:HIS360 2.1 71.9 1.0 NE2 B:HIS356 2.1 71.0 1.0 OE1 B:GLU384 2.6 74.1 1.0 CD B:GLU384 2.6 73.4 1.0 O B:HOH702 2.7 45.4 1.0 CE1 B:HIS356 3.0 69.5 1.0 CD2 B:HIS360 3.0 70.8 1.0 CE1 B:HIS360 3.1 70.8 1.0 CD2 B:HIS356 3.2 69.7 1.0 OG B:SER387 3.7 75.1 1.0 CE2 B:TYR490 3.8 69.2 1.0 OH B:TYR490 4.1 69.1 1.0 CG B:GLU384 4.1 72.7 1.0 ND1 B:HIS356 4.2 69.3 1.0 ND1 B:HIS360 4.2 70.7 1.0 CG B:HIS360 4.2 70.1 1.0 OH B:TYR486 4.2 71.3 1.0 CB B:SER387 4.3 75.5 1.0 CG B:HIS356 4.3 69.4 1.0 OE2 B:GLU357 4.4 71.6 1.0 CZ B:TYR490 4.4 69.3 1.0 OE1 B:GLU357 4.6 72.2 1.0 CD B:GLU357 4.8 71.4 1.0 CD2 B:TYR490 4.8 69.2 1.0

C.J.Gerdts, V.Tereshko, M.K.Yadav, I.Dementieva, F.Collart, A.Joachimiak, R.C.Stevens, P.Kuhn, A.Kossiakoff, R.F.Ismagilov. Time-Controlled Microfluidic Seeding in Nl-Volume Droplets to Separate Nucleation and Growth Stages of Protein Crystallization. Angew.Chem.Int.Ed.Engl. V. 45 8156 2006.
ISSN: ISSN 1433-7851
PubMed: 17099920
DOI: 10.1002/ANIE.200602946