Zinc in PDB 2gzj: Crystal Structure of the E9 Dnase Domain with A Mutant Immunity Protein IM9 (D51A)

All present enzymatic activity of Crystal Structure of the E9 Dnase Domain with A Mutant Immunity Protein IM9 (D51A):
3.1.21.1;

The structure of Crystal Structure of the E9 Dnase Domain with A Mutant Immunity Protein IM9 (D51A), PDB code: 2gzj was solved by P.S.Santi, O.O.Kolade, U.C.Kuhlmann, C.Kleanthous, A.M.Hemmings, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	19.86 / 1.60
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	44.903, 88.248, 52.235, 90.00, 91.92, 90.00
R / Rfree (%)	18.5 / 23.2

The binding sites of Zinc atom in the Crystal Structure of the E9 Dnase Domain with A Mutant Immunity Protein IM9 (D51A) (pdb code 2gzj). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Crystal Structure of the E9 Dnase Domain with A Mutant Immunity Protein IM9 (D51A), PDB code: 2gzj:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Crystal Structure of the E9 Dnase Domain with A Mutant Immunity Protein IM9 (D51A)


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Crystal Structure of the E9 Dnase Domain with A Mutant Immunity Protein IM9 (D51A) within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn301 b:12.9 occ:1.00 O3 B:PO4703 1.8 17.0 1.0 ND1 B:HIS102 2.1 8.0 1.0 NE2 B:HIS127 2.1 10.0 1.0 NE2 B:HIS131 2.1 8.3 1.0 CE1 B:HIS102 3.0 5.8 1.0 CD2 B:HIS127 3.0 10.5 1.0 CD2 B:HIS131 3.0 10.7 1.0 CE1 B:HIS131 3.1 10.3 1.0 P B:PO4703 3.1 18.1 1.0 CE1 B:HIS127 3.1 10.7 1.0 CG B:HIS102 3.1 6.2 1.0 O1 B:PO4703 3.4 27.9 1.0 CB B:HIS102 3.5 7.0 1.0 CA B:HIS102 3.7 6.9 1.0 O2 B:PO4703 3.7 26.3 1.0 O B:HOH716 3.9 17.2 1.0 N B:HIS103 4.0 6.2 1.0 NE2 B:HIS102 4.1 6.8 1.0 CG B:HIS127 4.2 10.5 1.0 ND1 B:HIS127 4.2 9.9 1.0 ND1 B:HIS131 4.2 12.6 1.0 CG B:HIS131 4.2 12.6 1.0 CD2 B:HIS102 4.2 7.0 1.0 O4 B:PO4703 4.3 21.1 1.0 C B:HIS102 4.3 6.8 1.0 O B:HIS103 4.5 5.4 1.0 O B:LEU101 4.8 9.7 1.0 N B:HIS102 4.9 7.4 1.0

Zinc binding site 2 out of 2 in the Crystal Structure of the E9 Dnase Domain with A Mutant Immunity Protein IM9 (D51A)


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Crystal Structure of the E9 Dnase Domain with A Mutant Immunity Protein IM9 (D51A) within 5.0Å range: probe atom residue distance (Å) B Occ F:Zn301 b:21.8 occ:1.00 O2 F:PO4704 1.9 21.9 1.0 ND1 F:HIS102 2.1 13.0 1.0 NE2 F:HIS131 2.1 22.6 1.0 NE2 F:HIS127 2.1 14.2 1.0 CE1 F:HIS102 2.9 10.7 1.0 CD2 F:HIS127 3.0 16.3 1.0 CD2 F:HIS131 3.0 24.8 1.0 P F:PO4704 3.1 24.2 1.0 CE1 F:HIS131 3.1 25.7 1.0 CG F:HIS102 3.1 10.8 1.0 CE1 F:HIS127 3.2 16.1 1.0 O4 F:PO4704 3.4 28.4 1.0 O1 F:PO4704 3.5 26.3 1.0 CB F:HIS102 3.6 9.7 1.0 CA F:HIS102 3.7 8.9 1.0 O F:HOH759 3.9 30.6 1.0 N F:HIS103 4.0 8.0 1.0 NE2 F:HIS102 4.1 14.2 1.0 CG F:HIS127 4.2 15.7 1.0 CG F:HIS131 4.2 25.4 1.0 CD2 F:HIS102 4.2 11.5 1.0 ND1 F:HIS131 4.2 25.1 1.0 ND1 F:HIS127 4.3 15.0 1.0 O F:HIS103 4.3 8.4 1.0 C F:HIS102 4.3 8.9 1.0 O3 F:PO4704 4.4 23.5 1.0

U.C.Kuhlmann, P.S.Santi, O.O.Kolade, C.Kleanthous, A.M.Hemmings. Crystal Structure of the Complex of the Colicin E9 Dnase Domain with A Mutant Immunity Protein, IM9 (D51A) To Be Published.