Zinc in PDB 2gu2: Crystal Structure of An Aspartoacylase From Rattus Norvegicus

All present enzymatic activity of Crystal Structure of An Aspartoacylase From Rattus Norvegicus:
3.5.1.15;

The structure of Crystal Structure of An Aspartoacylase From Rattus Norvegicus, PDB code: 2gu2 was solved by E.Bitto, G.E.Wesenberg, G.N.Phillips Jr., C.A.Bingman, Center Foreukaryotic Structural Genomics (Cesg), with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	41.75 / 1.81
Space group	C 1 2 1
Cell size a, b, c (Å), α, β, γ (°)	92.581, 135.778, 54.033, 90.00, 101.49, 90.00
R / Rfree (%)	14.9 / 19.4

The binding sites of Zinc atom in the Crystal Structure of An Aspartoacylase From Rattus Norvegicus (pdb code 2gu2). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Crystal Structure of An Aspartoacylase From Rattus Norvegicus, PDB code: 2gu2:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Crystal Structure of An Aspartoacylase From Rattus Norvegicus


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Crystal Structure of An Aspartoacylase From Rattus Norvegicus within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn601 b:11.1 occ:0.25 OE1 A:GLU23 2.2 13.1 1.0 ND1 A:HIS20 2.2 14.5 1.0 ND1 A:HIS115 2.3 16.8 1.0 OE2 A:GLU23 2.4 12.1 1.0 CD A:GLU23 2.6 12.4 1.0 O A:HOH748 2.7 32.8 1.0 CE1 A:HIS20 2.9 15.9 1.0 O A:HOH778 3.2 25.3 1.0 CG A:HIS115 3.3 14.2 1.0 CE1 A:HIS115 3.3 26.5 1.0 CG A:HIS20 3.5 13.7 1.0 CB A:HIS115 3.5 12.7 1.0 O A:HOH704 4.0 9.0 1.0 CB A:HIS20 4.0 11.3 1.0 O A:ASN116 4.1 13.8 1.0 CG A:GLU23 4.1 9.9 1.0 NH1 A:ARG62 4.1 11.4 1.0 NE2 A:HIS20 4.2 11.5 1.0 CA A:HIS115 4.3 11.4 1.0 CD2 A:HIS115 4.4 16.6 1.0 NE2 A:HIS115 4.4 18.4 1.0 CD2 A:HIS20 4.4 12.9 1.0 N A:ASN116 4.5 10.5 1.0 CB A:GLU23 4.8 12.2 1.0 C A:HIS115 4.9 8.6 1.0 OE1 A:GLU177 4.9 26.8 1.0 O A:HOH722 5.0 11.7 1.0

Zinc binding site 2 out of 2 in the Crystal Structure of An Aspartoacylase From Rattus Norvegicus


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Crystal Structure of An Aspartoacylase From Rattus Norvegicus within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn602 b:15.4 occ:0.25 ND1 B:HIS115 2.2 18.2 1.0 ND1 B:HIS20 2.2 16.0 1.0 OE1 B:GLU23 2.3 11.9 1.0 OE2 B:GLU23 2.5 14.0 1.0 CD B:GLU23 2.7 15.4 1.0 O B:HOH733 3.0 25.9 1.0 CE1 B:HIS20 3.0 15.6 1.0 CE1 B:HIS115 3.1 18.0 1.0 CG B:HIS115 3.2 20.0 1.0 CG B:HIS20 3.3 15.5 1.0 O B:HOH759 3.4 23.7 1.0 CB B:HIS115 3.6 13.4 1.0 CB B:HIS20 3.7 9.4 1.0 O B:HOH722 3.8 11.7 1.0 NH1 B:ARG62 4.1 15.6 1.0 CG B:GLU23 4.2 10.7 1.0 NE2 B:HIS20 4.2 16.9 1.0 NE2 B:HIS115 4.2 18.3 1.0 CD2 B:HIS115 4.3 18.3 1.0 CA B:HIS115 4.3 10.1 1.0 O B:ASN116 4.3 12.6 1.0 CD2 B:HIS20 4.4 15.5 1.0 O3 B:SO4704 4.4 50.2 1.0 N B:ASN116 4.7 12.0 1.0 O B:HOH716 4.9 13.0 1.0 CB B:GLU23 4.9 13.8 1.0 O B:HOH942 4.9 46.6 1.0

E.Bitto, C.A.Bingman, G.E.Wesenberg, J.G.Mccoy, G.N.Phillips. Structure of Aspartoacylase, the Brain Enzyme Impaired in Canavan Disease. Proc.Natl.Acad.Sci.Usa V. 104 456 2007.
ISSN: ISSN 0027-8424
PubMed: 17194761
DOI: 10.1073/PNAS.0607817104