Atomistry » Zinc » PDB 2g2n-2gc3 » 2ga3
Atomistry »
  Zinc »
    PDB 2g2n-2gc3 »
      2ga3 »

Zinc in PDB 2ga3: Structure of S102T E. Coli Alkaline Phosphatase-Phosphate Intermediate at 2.20A Resolution

Enzymatic activity of Structure of S102T E. Coli Alkaline Phosphatase-Phosphate Intermediate at 2.20A Resolution

All present enzymatic activity of Structure of S102T E. Coli Alkaline Phosphatase-Phosphate Intermediate at 2.20A Resolution:
3.1.3.1;

Protein crystallography data

The structure of Structure of S102T E. Coli Alkaline Phosphatase-Phosphate Intermediate at 2.20A Resolution, PDB code: 2ga3 was solved by J.Wang, E.R.Kantrowitz, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 14.98 / 2.20
Space group I 2 2 2
Cell size a, b, c (Å), α, β, γ (°) 76.230, 164.970, 192.900, 90.00, 90.00, 90.00
R / Rfree (%) 19.2 / 22.9

Other elements in 2ga3:

The structure of Structure of S102T E. Coli Alkaline Phosphatase-Phosphate Intermediate at 2.20A Resolution also contains other interesting chemical elements:

Magnesium (Mg) 2 atoms

Zinc Binding Sites:

The binding sites of Zinc atom in the Structure of S102T E. Coli Alkaline Phosphatase-Phosphate Intermediate at 2.20A Resolution (pdb code 2ga3). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 4 binding sites of Zinc where determined in the Structure of S102T E. Coli Alkaline Phosphatase-Phosphate Intermediate at 2.20A Resolution, PDB code: 2ga3:
Jump to Zinc binding site number: 1; 2; 3; 4;

Zinc binding site 1 out of 4 in 2ga3

Go back to Zinc Binding Sites List in 2ga3
Zinc binding site 1 out of 4 in the Structure of S102T E. Coli Alkaline Phosphatase-Phosphate Intermediate at 2.20A Resolution


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Structure of S102T E. Coli Alkaline Phosphatase-Phosphate Intermediate at 2.20A Resolution within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Zn450

b:39.9
occ:0.95
 O3P A:TPO102 1.8 38.9 1.0
NE2 A:HIS412 2.0 32.4 1.0
NE2 A:HIS331 2.1 43.5 1.0
OD2 A:ASP327 2.5 36.6 1.0
OD1 A:ASP327 2.5 37.0 1.0
CE1 A:HIS412 2.8 32.6 1.0
CG A:ASP327 2.9 38.7 1.0
CE1 A:HIS331 3.1 41.9 1.0
CD2 A:HIS331 3.1 42.1 1.0
CD2 A:HIS412 3.2 33.6 1.0
P A:TPO102 3.2 36.3 1.0
O1P A:TPO102 3.7 34.9 1.0
ZN A:ZN451 3.7 38.7 1.0
NE2 A:HIS370 3.8 29.5 1.0
CE1 A:HIS370 3.9 28.6 1.0
NE2 A:HIS372 3.9 35.6 1.0
ND1 A:HIS412 4.0 33.4 1.0
O2P A:TPO102 4.1 34.2 1.0
O A:HOH564 4.1 41.2 1.0
CG A:HIS412 4.2 32.9 1.0
OG1 A:TPO102 4.2 30.5 1.0
ND1 A:HIS331 4.2 42.8 1.0
CG A:HIS331 4.3 43.3 1.0
OD2 A:ASP51 4.3 32.7 1.0
CB A:ASP327 4.4 37.7 1.0
CD2 A:HIS372 4.6 34.8 1.0
CE1 A:HIS372 4.9 34.3 1.0
O A:ASP327 5.0 37.2 1.0

Zinc binding site 2 out of 4 in 2ga3

Go back to Zinc Binding Sites List in 2ga3
Zinc binding site 2 out of 4 in the Structure of S102T E. Coli Alkaline Phosphatase-Phosphate Intermediate at 2.20A Resolution


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Structure of S102T E. Coli Alkaline Phosphatase-Phosphate Intermediate at 2.20A Resolution within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Zn451

b:38.7
occ:1.00
 NE2 A:HIS370 1.9 29.5 1.0
OD2 A:ASP51 2.0 32.7 1.0
OD1 A:ASP369 2.1 26.8 1.0
OG1 A:TPO102 2.4 30.5 1.0
O3P A:TPO102 2.8 38.9 1.0
CE1 A:HIS370 2.8 28.6 1.0
CD2 A:HIS370 2.9 26.5 1.0
CG A:ASP51 3.0 33.0 1.0
CG A:ASP369 3.1 24.7 1.0
P A:TPO102 3.2 36.3 1.0
OD1 A:ASP51 3.4 38.2 1.0
OD1 A:ASP327 3.4 37.0 1.0
OD2 A:ASP369 3.4 23.9 1.0
CG A:ASP327 3.6 38.7 1.0
CB A:TPO102 3.6 27.4 1.0
ZN A:ZN450 3.7 39.9 0.9
ND1 A:HIS370 3.9 28.9 1.0
OD2 A:ASP327 3.9 36.6 1.0
CG A:HIS370 3.9 25.9 1.0
CG2 A:TPO102 4.0 26.4 1.0
N A:GLY52 4.0 22.7 1.0
CE1 A:HIS412 4.1 32.6 1.0
CA A:TPO102 4.2 24.6 1.0
CB A:ASP51 4.2 26.5 1.0
O1P A:TPO102 4.3 34.9 1.0
O2P A:TPO102 4.3 34.2 1.0
CB A:ASP327 4.3 37.7 1.0
NE2 A:HIS412 4.3 32.4 1.0
CB A:ASP369 4.4 23.1 1.0
C A:ASP51 4.5 23.6 1.0
N A:TPO102 4.5 24.9 1.0
CA A:ASP51 4.5 25.3 1.0
CA A:GLY52 4.6 21.6 1.0
ND1 A:HIS412 4.9 33.4 1.0
O A:HOH564 4.9 41.2 1.0

Zinc binding site 3 out of 4 in 2ga3

Go back to Zinc Binding Sites List in 2ga3
Zinc binding site 3 out of 4 in the Structure of S102T E. Coli Alkaline Phosphatase-Phosphate Intermediate at 2.20A Resolution


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 3 of Structure of S102T E. Coli Alkaline Phosphatase-Phosphate Intermediate at 2.20A Resolution within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Zn450

b:44.9
occ:0.95
 O2P B:TPO102 1.8 42.8 1.0
NE2 B:HIS331 2.0 46.3 1.0
NE2 B:HIS412 2.0 29.6 1.0
OD1 B:ASP327 2.4 38.6 1.0
OD2 B:ASP327 2.5 36.6 1.0
CG B:ASP327 2.8 38.1 1.0
CE1 B:HIS412 2.9 30.9 1.0
CD2 B:HIS331 3.0 43.2 1.0
CE1 B:HIS331 3.1 44.8 1.0
CD2 B:HIS412 3.1 33.1 1.0
P B:TPO102 3.2 39.8 1.0
ZN B:ZN451 3.7 43.8 1.0
O3P B:TPO102 3.7 38.9 1.0
NE2 B:HIS372 3.9 32.5 1.0
O1P B:TPO102 3.9 40.3 1.0
NE2 B:HIS370 3.9 32.8 1.0
CE1 B:HIS370 4.0 29.7 1.0
ND1 B:HIS412 4.1 34.2 1.0
CG B:HIS331 4.1 42.7 1.0
ND1 B:HIS331 4.2 44.1 1.0
CG B:HIS412 4.2 32.7 1.0
O B:HOH1094 4.2 41.9 1.0
OG1 B:TPO102 4.3 34.8 1.0
CB B:ASP327 4.3 37.1 1.0
OD1 B:ASP51 4.4 33.8 1.0
CD2 B:HIS372 4.5 32.1 1.0
CE1 B:HIS372 4.9 32.0 1.0
O B:ASP327 4.9 38.2 1.0

Zinc binding site 4 out of 4 in 2ga3

Go back to Zinc Binding Sites List in 2ga3
Zinc binding site 4 out of 4 in the Structure of S102T E. Coli Alkaline Phosphatase-Phosphate Intermediate at 2.20A Resolution


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 4 of Structure of S102T E. Coli Alkaline Phosphatase-Phosphate Intermediate at 2.20A Resolution within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Zn451

b:43.8
occ:1.00
 OD1 B:ASP51 2.0 33.8 1.0
NE2 B:HIS370 2.0 32.8 1.0
OD1 B:ASP369 2.1 29.6 1.0
OG1 B:TPO102 2.5 34.8 1.0
O2P B:TPO102 2.7 42.8 1.0
CE1 B:HIS370 2.8 29.7 1.0
CG B:ASP51 3.0 32.8 1.0
CG B:ASP369 3.0 28.9 1.0
CD2 B:HIS370 3.0 30.0 1.0
P B:TPO102 3.2 39.8 1.0
OD2 B:ASP369 3.3 29.1 1.0
OD2 B:ASP51 3.3 38.4 1.0
OD1 B:ASP327 3.4 38.6 1.0
CG B:ASP327 3.6 38.1 1.0
ZN B:ZN450 3.7 44.9 0.9
CB B:TPO102 3.7 31.2 1.0
ND1 B:HIS370 3.9 29.7 1.0
CE1 B:HIS412 4.0 30.9 1.0
OD2 B:ASP327 4.0 36.6 1.0
CG B:HIS370 4.0 29.2 1.0
N B:GLY52 4.1 24.5 1.0
CG2 B:TPO102 4.1 29.1 1.0
CA B:TPO102 4.1 29.1 1.0
NE2 B:HIS412 4.1 29.6 1.0
O3P B:TPO102 4.2 38.9 1.0
CB B:ASP51 4.3 28.3 1.0
CB B:ASP327 4.3 37.1 1.0
O1P B:TPO102 4.3 40.3 1.0
CB B:ASP369 4.3 27.3 1.0
N B:TPO102 4.3 28.1 1.0
CA B:ASP51 4.6 25.8 1.0
C B:ASP51 4.6 24.9 1.0
CA B:GLY52 4.7 23.2 1.0
ND1 B:HIS412 4.8 34.2 1.0
O B:HOH961 4.8 40.5 1.0

Reference:

J.Wang, E.R.Kantrowitz. Trapping the Tetrahedral Intermediate in the Alkaline Phosphatase Reaction By Substitution of the Active Site Serine with Threonine. Protein Sci. V. 15 2395 2006.
ISSN: ISSN 0961-8368
PubMed: 17008720
DOI: 10.1110/PS.062351506
Page generated: Thu Oct 17 00:06:37 2024

Last articles

Zn in 9JYW
Zn in 9IR4
Zn in 9IR3
Zn in 9GMX
Zn in 9GMW
Zn in 9JEJ
Zn in 9ERF
Zn in 9ERE
Zn in 9EGV
Zn in 9EGW
© Copyright 2008-2020 by atomistry.com
Home   |    Site Map   |    Copyright   |    Contact us   |    Privacy