Zinc in PDB 2g4z: Anomalous Substructure of Thermolysin

All present enzymatic activity of Anomalous Substructure of Thermolysin:
3.4.24.27;

The structure of Anomalous Substructure of Thermolysin, PDB code: 2g4z was solved by C.Mueller-Dieckmann, M.S.Weiss, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	30.00 / 1.98
Space group	P 61 2 2
Cell size a, b, c (Å), α, β, γ (°)	92.487, 92.487, 127.529, 90.00, 90.00, 120.00
R / Rfree (%)	19 / 23.7

The structure of Anomalous Substructure of Thermolysin also contains other interesting chemical elements:

Chlorine	(Cl)	8 atoms
Calcium	(Ca)	3 atoms

The binding sites of Zinc atom in the Anomalous Substructure of Thermolysin (pdb code 2g4z). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total only one binding site of Zinc was determined in the Anomalous Substructure of Thermolysin, PDB code: 2g4z:

Zinc binding site 1 out of 1 in the Anomalous Substructure of Thermolysin


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Anomalous Substructure of Thermolysin within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn321 b:36.6 occ:1.00 OE2 A:GLU166 2.0 34.6 1.0 NE2 A:HIS142 2.0 33.2 1.0 NE2 A:HIS146 2.1 38.9 1.0 CL A:CL326 2.4 44.8 0.7 CD A:GLU166 2.7 34.6 1.0 OE1 A:GLU166 2.8 31.7 1.0 CE1 A:HIS142 2.9 34.5 1.0 CD2 A:HIS142 3.0 34.4 1.0 CE1 A:HIS146 3.0 34.1 1.0 CD2 A:HIS146 3.2 37.2 1.0 OH A:TYR157 3.8 38.7 1.0 ND1 A:HIS142 4.0 31.2 1.0 NE2 A:HIS231 4.1 40.3 1.0 CG A:HIS142 4.1 31.1 1.0 ND1 A:HIS146 4.2 30.7 1.0 CG A:GLU166 4.2 37.5 1.0 CG A:HIS146 4.3 31.8 1.0 CB A:SER169 4.5 34.1 1.0 OE1 A:GLU143 4.5 34.9 1.0 CD2 A:HIS231 4.6 36.8 1.0 O A:HOH421 4.7 37.9 1.0 OG A:SER169 4.7 33.5 1.0 CZ A:TYR157 4.8 45.4 1.0 CA A:GLU166 4.9 34.8 1.0 CE1 A:TYR157 5.0 44.5 1.0

C.Mueller-Dieckmann, S.Panjikar, A.Schmidt, S.Mueller, J.Kuper, A.Geerlof, M.Wilmanns, R.K.Singh, P.A.Tucker, M.S.Weiss. On the Routine Use of Soft X-Rays in Macromolecular Crystallography. Part IV. Efficient Determination of Anomalous Substructures in Biomacromolecules Using Longer X-Ray Wavelengths. Acta Crystallogr.,Sect.D V. 63 366 2007.
ISSN: ISSN 0907-4449
PubMed: 17327674
DOI: 10.1107/S0907444906055624