Zinc in PDB 2ftw: Crystal Structure of Dihydropyrimidinase From Dictyostelium Discoideum

All present enzymatic activity of Crystal Structure of Dihydropyrimidinase From Dictyostelium Discoideum:
3.5.2.2;

The structure of Crystal Structure of Dihydropyrimidinase From Dictyostelium Discoideum, PDB code: 2ftw was solved by B.Lohkamp, D.Dobritzsch, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	25.00 / 2.05
Space group	I 2 2 2
Cell size a, b, c (Å), α, β, γ (°)	84.600, 89.599, 134.953, 90.00, 90.00, 90.00
R / Rfree (%)	20.4 / 25.2

The binding sites of Zinc atom in the Crystal Structure of Dihydropyrimidinase From Dictyostelium Discoideum (pdb code 2ftw). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Crystal Structure of Dihydropyrimidinase From Dictyostelium Discoideum, PDB code: 2ftw:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Crystal Structure of Dihydropyrimidinase From Dictyostelium Discoideum


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Crystal Structure of Dihydropyrimidinase From Dictyostelium Discoideum within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn522 b:41.7 occ:0.50 NE2 A:HIS68 2.2 26.2 1.0 NE2 A:HIS66 2.2 30.1 1.0 OQ1 A:KCX158 2.6 25.6 0.5 OD1 A:ASP325 2.6 32.4 1.0 O A:HOH762 2.8 42.5 1.0 CD2 A:HIS68 3.0 24.9 1.0 CE1 A:HIS66 3.1 29.9 1.0 CE1 A:HIS68 3.2 25.4 1.0 CG A:ASP325 3.3 27.6 1.0 CD2 A:HIS66 3.3 28.3 1.0 CX A:KCX158 3.4 26.0 0.5 OD2 A:ASP325 3.5 32.2 1.0 OQ2 A:KCX158 3.6 26.4 0.5 ZN A:ZN523 3.9 36.6 0.2 CD2 A:HIS247 4.0 23.7 1.0 CG A:HIS68 4.2 24.3 1.0 ND1 A:HIS66 4.2 29.2 1.0 ND1 A:HIS68 4.3 24.9 1.0 NE2 A:HIS247 4.3 25.6 1.0 CG A:HIS66 4.3 26.9 1.0 CB A:ASP325 4.4 26.8 1.0 NZ A:KCX158 4.5 25.9 1.0 CA A:ASP325 4.7 25.9 1.0

Zinc binding site 2 out of 2 in the Crystal Structure of Dihydropyrimidinase From Dictyostelium Discoideum


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Crystal Structure of Dihydropyrimidinase From Dictyostelium Discoideum within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn523 b:36.6 occ:0.25 OQ2 A:KCX158 1.8 26.4 0.5 ND1 A:HIS191 2.1 22.6 1.0 NE2 A:HIS247 2.4 25.6 1.0 CX A:KCX158 2.9 26.0 0.5 CE1 A:HIS191 3.0 20.9 1.0 CG A:HIS191 3.0 20.8 1.0 CE1 A:HIS247 3.2 24.7 1.0 CB A:HIS191 3.4 20.2 1.0 CD2 A:HIS247 3.5 23.7 1.0 OQ1 A:KCX158 3.5 25.6 0.5 CE2 A:PHE160 3.9 26.4 1.0 ZN A:ZN522 3.9 41.7 0.5 NZ A:KCX158 3.9 25.9 1.0 CE A:KCX158 4.0 26.1 1.0 NE2 A:HIS191 4.1 21.6 1.0 O A:HOH762 4.1 42.5 1.0 CD2 A:HIS191 4.1 22.2 1.0 CG2 A:VAL246 4.4 21.2 1.0 ND1 A:HIS247 4.4 24.5 1.0 CA A:HIS191 4.5 20.1 1.0 O A:GLY297 4.5 24.3 1.0 CD2 A:PHE160 4.5 27.2 1.0 CG A:HIS247 4.6 22.9 1.0 CZ A:PHE160 4.7 26.1 1.0 OH A:TYR163 4.7 34.8 1.0 CE1 A:HIS66 4.9 29.9 1.0

B.Lohkamp, B.Andersen, J.Piskur, D.Dobritzsch. The Crystal Structures of Dihydropyrimidinases Reaffirm the Close Relationship Between Cyclic Amidohydrolases and Explain Their Substrate Specificity. J.Biol.Chem. V. 281 13762 2006.
ISSN: ISSN 0021-9258
PubMed: 16517602
DOI: 10.1074/JBC.M513266200