Zinc in PDB 2fpx: Crystal Structure of the N-Terminal Domain of E.Coli Hisb- Sulfate Complex.

All present enzymatic activity of Crystal Structure of the N-Terminal Domain of E.Coli Hisb- Sulfate Complex.:
3.1.3.15;

The structure of Crystal Structure of the N-Terminal Domain of E.Coli Hisb- Sulfate Complex., PDB code: 2fpx was solved by E.S.Rangarajan, M.Cygler, A.Matte, Montreal-Kingston Bacterialstructural Genomics Initiative (Bsgi), with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	50.00 / 1.80
Space group	C 2 2 21
Cell size a, b, c (Å), α, β, γ (°)	53.605, 132.778, 107.162, 90.00, 90.00, 90.00
R / Rfree (%)	17.8 / 21.3

The structure of Crystal Structure of the N-Terminal Domain of E.Coli Hisb- Sulfate Complex. also contains other interesting chemical elements:

Magnesium

(Mg)

3 atoms

The binding sites of Zinc atom in the Crystal Structure of the N-Terminal Domain of E.Coli Hisb- Sulfate Complex. (pdb code 2fpx). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Crystal Structure of the N-Terminal Domain of E.Coli Hisb- Sulfate Complex., PDB code: 2fpx:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Crystal Structure of the N-Terminal Domain of E.Coli Hisb- Sulfate Complex.


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Crystal Structure of the N-Terminal Domain of E.Coli Hisb- Sulfate Complex. within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn602 b:23.5 occ:1.00 ND1 A:HIS96 2.1 23.2 1.0 SG A:CYS104 2.2 24.9 1.0 SG A:CYS94 2.3 19.5 1.0 SG A:CYS102 2.4 27.0 1.0 CE1 A:HIS96 3.0 24.2 1.0 CG A:HIS96 3.2 22.2 1.0 CB A:CYS102 3.3 28.9 1.0 CB A:CYS94 3.5 19.7 1.0 CB A:CYS104 3.5 25.1 1.0 CB A:HIS96 3.6 22.0 1.0 N A:ARG105 3.6 23.8 1.0 C A:CYS104 3.8 24.6 1.0 N A:CYS104 3.8 26.2 1.0 CA A:CYS104 3.8 25.2 1.0 CA A:CYS94 4.1 19.5 1.0 NE2 A:HIS96 4.1 23.7 1.0 N A:HIS96 4.2 21.3 1.0 CD2 A:HIS96 4.2 23.7 1.0 CA A:ARG105 4.3 23.2 1.0 CD A:PRO95 4.3 19.5 1.0 CB A:ARG105 4.4 23.3 1.0 O A:CYS104 4.4 24.6 1.0 CA A:HIS96 4.5 22.0 1.0 C A:CYS94 4.5 19.6 1.0 N A:PRO95 4.5 19.6 1.0 O A:HOH650 4.6 25.0 1.0 CA A:CYS102 4.7 28.8 1.0 N A:ASP103 4.8 28.4 1.0 C A:CYS102 4.9 28.6 1.0

Zinc binding site 2 out of 2 in the Crystal Structure of the N-Terminal Domain of E.Coli Hisb- Sulfate Complex.


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Crystal Structure of the N-Terminal Domain of E.Coli Hisb- Sulfate Complex. within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn601 b:11.9 occ:1.00 ND1 B:HIS96 2.1 10.2 1.0 SG B:CYS104 2.3 11.3 1.0 SG B:CYS94 2.3 9.1 1.0 SG B:CYS102 2.4 11.0 1.0 CE1 B:HIS96 3.1 11.0 1.0 CG B:HIS96 3.1 9.6 1.0 CB B:CYS102 3.3 10.9 1.0 CB B:CYS94 3.4 8.9 1.0 CB B:HIS96 3.4 10.0 1.0 CB B:CYS104 3.5 10.5 1.0 N B:ARG105 3.5 10.5 1.0 C B:CYS104 3.7 10.5 1.0 CA B:CYS104 3.8 10.3 1.0 N B:CYS104 3.8 10.2 1.0 CA B:CYS94 4.1 8.7 1.0 N B:HIS96 4.2 10.3 1.0 CA B:ARG105 4.2 10.3 1.0 NE2 B:HIS96 4.2 10.7 1.0 CD B:PRO95 4.2 9.3 1.0 CD2 B:HIS96 4.3 10.8 1.0 CB B:ARG105 4.3 10.6 1.0 O B:CYS104 4.4 10.2 1.0 CA B:HIS96 4.5 10.3 1.0 O B:HOH637 4.5 19.6 1.0 C B:CYS94 4.5 8.7 1.0 N B:PRO95 4.6 9.2 1.0 CA B:CYS102 4.6 10.6 1.0 C B:CYS102 4.8 10.8 1.0 N B:ASP103 4.9 10.4 1.0 O B:HOH850 5.0 31.1 1.0

E.S.Rangarajan, A.Proteau, J.Wagner, M.N.Hung, A.Matte, M.Cygler. Structural Snapshots of Escherichia Coli Histidinol Phosphate Phosphatase Along the Reaction Pathway. J.Biol.Chem. V. 281 37930 2006.
ISSN: ISSN 0021-9258
PubMed: 16966333
DOI: 10.1074/JBC.M604916200