Zinc in PDB 2f7o: Golgi Alpha-Mannosidase II Complex with Mannostatin A

All present enzymatic activity of Golgi Alpha-Mannosidase II Complex with Mannostatin A:
3.2.1.114;

The structure of Golgi Alpha-Mannosidase II Complex with Mannostatin A, PDB code: 2f7o was solved by D.A.Kuntz, D.R.Rose, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	29.75 / 1.43
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	69.002, 109.301, 138.352, 90.00, 90.00, 90.00
R / Rfree (%)	20.6 / 23.5

The binding sites of Zinc atom in the Golgi Alpha-Mannosidase II Complex with Mannostatin A (pdb code 2f7o). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total only one binding site of Zinc was determined in the Golgi Alpha-Mannosidase II Complex with Mannostatin A, PDB code: 2f7o:

Zinc binding site 1 out of 1 in the Golgi Alpha-Mannosidase II Complex with Mannostatin A


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Golgi Alpha-Mannosidase II Complex with Mannostatin A within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn5001 b:8.4 occ:1.00 OD2 A:ASP204 2.1 6.9 1.0 NE2 A:HIS471 2.1 5.2 1.0 NE2 A:HIS90 2.1 6.6 1.0 OD1 A:ASP92 2.1 9.1 1.0 O3 A:MSN5002 2.2 8.5 1.0 O4 A:MSN5002 2.2 9.4 1.0 CD2 A:HIS471 3.0 7.0 1.0 CD2 A:HIS90 3.0 5.9 1.0 CG A:ASP92 3.1 9.4 1.0 C4 A:MSN5002 3.1 8.6 1.0 C3 A:MSN5002 3.1 10.6 1.0 CE1 A:HIS471 3.1 6.2 1.0 CG A:ASP204 3.1 7.1 1.0 CE1 A:HIS90 3.2 8.0 1.0 OD2 A:ASP92 3.4 10.8 1.0 CB A:ASP204 3.5 7.4 1.0 N5 A:MSN5002 3.9 9.3 1.0 C5 A:MSN5002 4.0 9.5 1.0 OD2 A:ASP472 4.0 7.8 1.0 C1 A:MSN5002 4.1 8.5 1.0 CG A:HIS471 4.2 5.0 1.0 ND1 A:HIS471 4.2 5.3 1.0 C2 A:MSN5002 4.2 9.1 1.0 CG A:HIS90 4.2 6.8 1.0 ND1 A:HIS90 4.3 7.3 1.0 OD1 A:ASP204 4.3 7.2 1.0 CE1 A:HIS470 4.5 7.1 1.0 CB A:ASP92 4.5 7.0 1.0 OH A:TYR269 4.6 11.3 1.0 O A:HOH5849 4.7 9.6 1.0 O2 A:MSN5002 4.9 7.8 1.0

S.P.Kawatkar, D.A.Kuntz, R.J.Woods, D.R.Rose, G.J.Boons. Structural Basis of the Inhibition of Golgi Alpha-Mannosidase II By Mannostatin A and the Role of the Thiomethyl Moiety in Ligand-Protein Interactions. J.Am.Chem.Soc. V. 128 8310 2006.
ISSN: ISSN 0002-7863
PubMed: 16787095
DOI: 10.1021/JA061216P