Atomistry » Zinc » PDB 2cih-2ctb » 2cn6
Atomistry »
  Zinc »
    PDB 2cih-2ctb »
      2cn6 »

Zinc in PDB 2cn6: Recombinant Human H Ferritin, K86Q and E107D Mutant, Soaked with Zn Ions

Enzymatic activity of Recombinant Human H Ferritin, K86Q and E107D Mutant, Soaked with Zn Ions

All present enzymatic activity of Recombinant Human H Ferritin, K86Q and E107D Mutant, Soaked with Zn Ions:
1.16.3.1;

Protein crystallography data

The structure of Recombinant Human H Ferritin, K86Q and E107D Mutant, Soaked with Zn Ions, PDB code: 2cn6 was solved by L.Toussaint, R.R.Crichton, J.P.Declercq, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 103.14 / 2.20
Space group F 4 3 2
Cell size a, b, c (Å), α, β, γ (°) 178.453, 178.453, 178.453, 90.00, 90.00, 90.00
R / Rfree (%) 19.5 / 23

Other elements in 2cn6:

The structure of Recombinant Human H Ferritin, K86Q and E107D Mutant, Soaked with Zn Ions also contains other interesting chemical elements:

Calcium (Ca) 1 atom

Zinc Binding Sites:

The binding sites of Zinc atom in the Recombinant Human H Ferritin, K86Q and E107D Mutant, Soaked with Zn Ions (pdb code 2cn6). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 7 binding sites of Zinc where determined in the Recombinant Human H Ferritin, K86Q and E107D Mutant, Soaked with Zn Ions, PDB code: 2cn6:
Jump to Zinc binding site number: 1; 2; 3; 4; 5; 6; 7;

Zinc binding site 1 out of 7 in 2cn6

Go back to Zinc Binding Sites List in 2cn6
Zinc binding site 1 out of 7 in the Recombinant Human H Ferritin, K86Q and E107D Mutant, Soaked with Zn Ions


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Recombinant Human H Ferritin, K86Q and E107D Mutant, Soaked with Zn Ions within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Zn210

b:32.5
occ:0.50
 OE1 A:GLN86 2.1 34.9 1.0
CD A:GLN86 3.2 34.9 1.0
NE2 A:GLN86 3.7 33.9 1.0
O A:HOH2046 4.1 25.8 1.0
CG A:GLN86 4.5 27.8 1.0
CB A:GLN86 4.8 26.0 1.0
CA A:GLN86 4.8 25.9 1.0
N A:LYS87 4.9 26.6 1.0

Zinc binding site 2 out of 7 in 2cn6

Go back to Zinc Binding Sites List in 2cn6
Zinc binding site 2 out of 7 in the Recombinant Human H Ferritin, K86Q and E107D Mutant, Soaked with Zn Ions


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Recombinant Human H Ferritin, K86Q and E107D Mutant, Soaked with Zn Ions within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Zn212

b:52.4
occ:1.00
 OE1 A:GLU27 2.1 37.9 1.0
OE1 A:GLU62 2.1 35.9 1.0
ND1 A:HIS65 2.5 39.0 1.0
CD A:GLU27 3.1 33.2 1.0
CD A:GLU62 3.2 35.4 1.0
CE1 A:HIS65 3.4 40.4 1.0
ZN A:ZN213 3.4 74.5 1.0
OE2 A:GLU27 3.4 39.1 1.0
OE2 A:GLU62 3.6 46.3 1.0
CG A:HIS65 3.6 33.0 1.0
CB A:HIS65 3.9 29.4 1.0
OE1 A:GLN141 4.0 47.6 1.0
CG1 A:VAL110 4.3 36.7 1.0
CG A:GLU27 4.4 28.4 1.0
CG A:GLU62 4.5 30.6 1.0
CA A:GLU62 4.5 28.3 1.0
NE2 A:HIS65 4.6 42.6 1.0
CB A:GLU62 4.7 27.7 1.0
CD2 A:HIS65 4.7 36.7 1.0
CB A:GLU27 4.7 24.8 1.0
CA A:GLU27 5.0 24.4 1.0
CD A:GLN141 5.0 46.6 1.0

Zinc binding site 3 out of 7 in 2cn6

Go back to Zinc Binding Sites List in 2cn6
Zinc binding site 3 out of 7 in the Recombinant Human H Ferritin, K86Q and E107D Mutant, Soaked with Zn Ions


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 3 of Recombinant Human H Ferritin, K86Q and E107D Mutant, Soaked with Zn Ions within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Zn213

b:74.5
occ:1.00
 OE2 A:GLU62 2.1 46.3 1.0
CD A:GLU62 3.0 35.4 1.0
OD1 A:ASP107 3.2 40.8 1.0
OE1 A:GLU62 3.2 35.9 1.0
O A:HOH2032 3.2 56.5 1.0
OD2 A:ASP107 3.2 47.4 1.0
ZN A:ZN212 3.4 52.4 1.0
CG A:ASP107 3.5 38.6 1.0
OE1 A:GLN141 3.6 47.6 1.0
NE2 A:GLN141 3.9 47.6 1.0
CD A:GLN141 4.1 46.6 1.0
CG A:GLU62 4.3 30.6 1.0
CE2 A:TYR34 4.5 30.5 1.0
CB A:ASP107 4.8 34.0 1.0
CE1 A:HIS65 4.8 40.4 1.0
OH A:TYR34 4.9 38.0 1.0
ND1 A:HIS65 4.9 39.0 1.0

Zinc binding site 4 out of 7 in 2cn6

Go back to Zinc Binding Sites List in 2cn6
Zinc binding site 4 out of 7 in the Recombinant Human H Ferritin, K86Q and E107D Mutant, Soaked with Zn Ions


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 4 of Recombinant Human H Ferritin, K86Q and E107D Mutant, Soaked with Zn Ions within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Zn214

b:45.2
occ:0.25
 O A:HOH2068 2.3 18.6 0.2
NE2 A:HIS173 2.3 41.9 1.0
CE1 A:HIS173 2.7 40.5 1.0
CD2 A:HIS173 3.6 35.1 1.0
ND1 A:HIS173 4.0 39.7 1.0
CG A:HIS173 4.5 33.9 1.0
CD2 A:LEU169 4.9 22.2 1.0

Zinc binding site 5 out of 7 in 2cn6

Go back to Zinc Binding Sites List in 2cn6
Zinc binding site 5 out of 7 in the Recombinant Human H Ferritin, K86Q and E107D Mutant, Soaked with Zn Ions


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 5 of Recombinant Human H Ferritin, K86Q and E107D Mutant, Soaked with Zn Ions within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Zn215

b:43.2
occ:1.00
 OE1 A:GLU67 2.1 35.0 1.0
OE2 A:GLU64 2.3 44.5 1.0
OE1 A:GLU64 2.5 41.8 1.0
CD A:GLU64 2.8 41.0 1.0
CD A:GLU67 2.8 29.0 1.0
OE2 A:GLU67 2.8 29.5 1.0
O A:HOH2035 4.2 60.4 1.0
CG A:GLU67 4.2 28.5 1.0
CG A:GLU64 4.3 34.4 1.0
CB A:GLU67 4.7 25.6 1.0
NE A:ARG63 4.9 37.6 1.0
NH2 A:ARG63 4.9 32.6 1.0
CA A:GLU64 5.0 30.0 1.0

Zinc binding site 6 out of 7 in 2cn6

Go back to Zinc Binding Sites List in 2cn6
Zinc binding site 6 out of 7 in the Recombinant Human H Ferritin, K86Q and E107D Mutant, Soaked with Zn Ions


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 6 of Recombinant Human H Ferritin, K86Q and E107D Mutant, Soaked with Zn Ions within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Zn216

b:61.6
occ:1.00
 NE2 A:HIS118 2.1 44.2 1.0
SG A:CYS130 2.3 47.0 1.0
OE2 A:GLU134 2.7 56.0 1.0
CE1 A:HIS118 2.8 45.1 1.0
CD2 A:HIS118 3.2 43.3 1.0
CB A:CYS130 3.2 43.7 1.0
ZN A:ZN220 3.3 72.7 1.0
CD A:GLU134 3.9 56.1 1.0
ND1 A:HIS118 3.9 44.7 1.0
OG1 A:THR122 4.0 49.5 1.0
O A:HOH2069 4.1 53.9 0.3
CG A:HIS118 4.1 43.6 1.0
CA A:CYS130 4.3 43.9 1.0
OE1 A:GLU134 4.5 58.2 1.0
C A:CYS130 4.7 44.0 1.0
O A:CYS130 4.8 43.2 1.0
CG A:GLU134 4.9 53.6 1.0

Zinc binding site 7 out of 7 in 2cn6

Go back to Zinc Binding Sites List in 2cn6
Zinc binding site 7 out of 7 in the Recombinant Human H Ferritin, K86Q and E107D Mutant, Soaked with Zn Ions


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 7 of Recombinant Human H Ferritin, K86Q and E107D Mutant, Soaked with Zn Ions within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Zn220

b:72.7
occ:1.00
 SG A:CYS130 2.4 47.0 1.0
O A:HOH2069 2.7 53.9 0.3
ZN A:ZN216 3.3 61.6 1.0
CB A:CYS130 3.6 43.7 1.0
OE2 A:GLU134 4.3 56.0 1.0
O A:PRO127 4.5 41.5 1.0
CA A:PRO127 4.5 41.5 1.0
OE1 A:GLU134 4.5 58.2 1.0
CB A:PRO127 4.6 41.8 1.0
N A:ASP131 4.7 44.4 1.0
CA A:CYS130 4.8 43.9 1.0
CD A:GLU134 4.8 56.1 1.0
C A:CYS130 4.8 44.0 1.0

Reference:

L.Toussaint, L.Bertrand, L.Hue, R.R.Crichton, J.P.Declercq. High-Resolution X-Ray Structures of Human Apoferritin H-Chain Mutants Correlated with Their Activity and Metal-Binding Sites. J.Mol.Biol. V. 365 440 2007.
ISSN: ISSN 0022-2836
PubMed: 17070541
DOI: 10.1016/J.JMB.2006.10.010
Page generated: Wed Oct 16 22:26:48 2024

Last articles

Zn in 9JYW
Zn in 9IR4
Zn in 9IR3
Zn in 9GMX
Zn in 9GMW
Zn in 9JEJ
Zn in 9ERF
Zn in 9ERE
Zn in 9EGV
Zn in 9EGW
© Copyright 2008-2020 by atomistry.com
Home   |    Site Map   |    Copyright   |    Contact us   |    Privacy