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Zinc in PDB 2cn6: Recombinant Human H Ferritin, K86Q and E107D Mutant, Soaked with Zn Ions

Enzymatic activity of Recombinant Human H Ferritin, K86Q and E107D Mutant, Soaked with Zn Ions

All present enzymatic activity of Recombinant Human H Ferritin, K86Q and E107D Mutant, Soaked with Zn Ions:
1.16.3.1;

Protein crystallography data

The structure of Recombinant Human H Ferritin, K86Q and E107D Mutant, Soaked with Zn Ions, PDB code: 2cn6 was solved by L.Toussaint, R.R.Crichton, J.P.Declercq, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 103.14 / 2.20
Space group F 4 3 2
Cell size a, b, c (Å), α, β, γ (°) 178.453, 178.453, 178.453, 90.00, 90.00, 90.00
R / Rfree (%) 19.5 / 23

Other elements in 2cn6:

The structure of Recombinant Human H Ferritin, K86Q and E107D Mutant, Soaked with Zn Ions also contains other interesting chemical elements:

Calcium (Ca) 1 atom

Zinc Binding Sites:

The binding sites of Zinc atom in the Recombinant Human H Ferritin, K86Q and E107D Mutant, Soaked with Zn Ions (pdb code 2cn6). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 7 binding sites of Zinc where determined in the Recombinant Human H Ferritin, K86Q and E107D Mutant, Soaked with Zn Ions, PDB code: 2cn6:
Jump to Zinc binding site number: 1; 2; 3; 4; 5; 6; 7;

Zinc binding site 1 out of 7 in 2cn6

Go back to Zinc Binding Sites List in 2cn6
Zinc binding site 1 out of 7 in the Recombinant Human H Ferritin, K86Q and E107D Mutant, Soaked with Zn Ions


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Recombinant Human H Ferritin, K86Q and E107D Mutant, Soaked with Zn Ions within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Zn210

b:32.5
occ:0.50
OE1 A:GLN86 2.1 34.9 1.0
CD A:GLN86 3.2 34.9 1.0
NE2 A:GLN86 3.7 33.9 1.0
O A:HOH2046 4.1 25.8 1.0
CG A:GLN86 4.5 27.8 1.0
CB A:GLN86 4.8 26.0 1.0
CA A:GLN86 4.8 25.9 1.0
N A:LYS87 4.9 26.6 1.0

Zinc binding site 2 out of 7 in 2cn6

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Zinc binding site 2 out of 7 in the Recombinant Human H Ferritin, K86Q and E107D Mutant, Soaked with Zn Ions


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Recombinant Human H Ferritin, K86Q and E107D Mutant, Soaked with Zn Ions within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Zn212

b:52.4
occ:1.00
OE1 A:GLU27 2.1 37.9 1.0
OE1 A:GLU62 2.1 35.9 1.0
ND1 A:HIS65 2.5 39.0 1.0
CD A:GLU27 3.1 33.2 1.0
CD A:GLU62 3.2 35.4 1.0
CE1 A:HIS65 3.4 40.4 1.0
ZN A:ZN213 3.4 74.5 1.0
OE2 A:GLU27 3.4 39.1 1.0
OE2 A:GLU62 3.6 46.3 1.0
CG A:HIS65 3.6 33.0 1.0
CB A:HIS65 3.9 29.4 1.0
OE1 A:GLN141 4.0 47.6 1.0
CG1 A:VAL110 4.3 36.7 1.0
CG A:GLU27 4.4 28.4 1.0
CG A:GLU62 4.5 30.6 1.0
CA A:GLU62 4.5 28.3 1.0
NE2 A:HIS65 4.6 42.6 1.0
CB A:GLU62 4.7 27.7 1.0
CD2 A:HIS65 4.7 36.7 1.0
CB A:GLU27 4.7 24.8 1.0
CA A:GLU27 5.0 24.4 1.0
CD A:GLN141 5.0 46.6 1.0

Zinc binding site 3 out of 7 in 2cn6

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Zinc binding site 3 out of 7 in the Recombinant Human H Ferritin, K86Q and E107D Mutant, Soaked with Zn Ions


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 3 of Recombinant Human H Ferritin, K86Q and E107D Mutant, Soaked with Zn Ions within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Zn213

b:74.5
occ:1.00
OE2 A:GLU62 2.1 46.3 1.0
CD A:GLU62 3.0 35.4 1.0
OD1 A:ASP107 3.2 40.8 1.0
OE1 A:GLU62 3.2 35.9 1.0
O A:HOH2032 3.2 56.5 1.0
OD2 A:ASP107 3.2 47.4 1.0
ZN A:ZN212 3.4 52.4 1.0
CG A:ASP107 3.5 38.6 1.0
OE1 A:GLN141 3.6 47.6 1.0
NE2 A:GLN141 3.9 47.6 1.0
CD A:GLN141 4.1 46.6 1.0
CG A:GLU62 4.3 30.6 1.0
CE2 A:TYR34 4.5 30.5 1.0
CB A:ASP107 4.8 34.0 1.0
CE1 A:HIS65 4.8 40.4 1.0
OH A:TYR34 4.9 38.0 1.0
ND1 A:HIS65 4.9 39.0 1.0

Zinc binding site 4 out of 7 in 2cn6

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Zinc binding site 4 out of 7 in the Recombinant Human H Ferritin, K86Q and E107D Mutant, Soaked with Zn Ions


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 4 of Recombinant Human H Ferritin, K86Q and E107D Mutant, Soaked with Zn Ions within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Zn214

b:45.2
occ:0.25
O A:HOH2068 2.3 18.6 0.2
NE2 A:HIS173 2.3 41.9 1.0
CE1 A:HIS173 2.7 40.5 1.0
CD2 A:HIS173 3.6 35.1 1.0
ND1 A:HIS173 4.0 39.7 1.0
CG A:HIS173 4.5 33.9 1.0
CD2 A:LEU169 4.9 22.2 1.0

Zinc binding site 5 out of 7 in 2cn6

Go back to Zinc Binding Sites List in 2cn6
Zinc binding site 5 out of 7 in the Recombinant Human H Ferritin, K86Q and E107D Mutant, Soaked with Zn Ions


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 5 of Recombinant Human H Ferritin, K86Q and E107D Mutant, Soaked with Zn Ions within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Zn215

b:43.2
occ:1.00
OE1 A:GLU67 2.1 35.0 1.0
OE2 A:GLU64 2.3 44.5 1.0
OE1 A:GLU64 2.5 41.8 1.0
CD A:GLU64 2.8 41.0 1.0
CD A:GLU67 2.8 29.0 1.0
OE2 A:GLU67 2.8 29.5 1.0
O A:HOH2035 4.2 60.4 1.0
CG A:GLU67 4.2 28.5 1.0
CG A:GLU64 4.3 34.4 1.0
CB A:GLU67 4.7 25.6 1.0
NE A:ARG63 4.9 37.6 1.0
NH2 A:ARG63 4.9 32.6 1.0
CA A:GLU64 5.0 30.0 1.0

Zinc binding site 6 out of 7 in 2cn6

Go back to Zinc Binding Sites List in 2cn6
Zinc binding site 6 out of 7 in the Recombinant Human H Ferritin, K86Q and E107D Mutant, Soaked with Zn Ions


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 6 of Recombinant Human H Ferritin, K86Q and E107D Mutant, Soaked with Zn Ions within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Zn216

b:61.6
occ:1.00
NE2 A:HIS118 2.1 44.2 1.0
SG A:CYS130 2.3 47.0 1.0
OE2 A:GLU134 2.7 56.0 1.0
CE1 A:HIS118 2.8 45.1 1.0
CD2 A:HIS118 3.2 43.3 1.0
CB A:CYS130 3.2 43.7 1.0
ZN A:ZN220 3.3 72.7 1.0
CD A:GLU134 3.9 56.1 1.0
ND1 A:HIS118 3.9 44.7 1.0
OG1 A:THR122 4.0 49.5 1.0
O A:HOH2069 4.1 53.9 0.3
CG A:HIS118 4.1 43.6 1.0
CA A:CYS130 4.3 43.9 1.0
OE1 A:GLU134 4.5 58.2 1.0
C A:CYS130 4.7 44.0 1.0
O A:CYS130 4.8 43.2 1.0
CG A:GLU134 4.9 53.6 1.0

Zinc binding site 7 out of 7 in 2cn6

Go back to Zinc Binding Sites List in 2cn6
Zinc binding site 7 out of 7 in the Recombinant Human H Ferritin, K86Q and E107D Mutant, Soaked with Zn Ions


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 7 of Recombinant Human H Ferritin, K86Q and E107D Mutant, Soaked with Zn Ions within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Zn220

b:72.7
occ:1.00
SG A:CYS130 2.4 47.0 1.0
O A:HOH2069 2.7 53.9 0.3
ZN A:ZN216 3.3 61.6 1.0
CB A:CYS130 3.6 43.7 1.0
OE2 A:GLU134 4.3 56.0 1.0
O A:PRO127 4.5 41.5 1.0
CA A:PRO127 4.5 41.5 1.0
OE1 A:GLU134 4.5 58.2 1.0
CB A:PRO127 4.6 41.8 1.0
N A:ASP131 4.7 44.4 1.0
CA A:CYS130 4.8 43.9 1.0
CD A:GLU134 4.8 56.1 1.0
C A:CYS130 4.8 44.0 1.0

Reference:

L.Toussaint, L.Bertrand, L.Hue, R.R.Crichton, J.P.Declercq. High-Resolution X-Ray Structures of Human Apoferritin H-Chain Mutants Correlated with Their Activity and Metal-Binding Sites. J.Mol.Biol. V. 365 440 2007.
ISSN: ISSN 0022-2836
PubMed: 17070541
DOI: 10.1016/J.JMB.2006.10.010
Page generated: Wed Oct 16 22:26:48 2024

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