Zinc in PDB 2c0b: Catalytic Domain of E. Coli Rnase E in Complex with 13-Mer Rna

The structure of Catalytic Domain of E. Coli Rnase E in Complex with 13-Mer Rna, PDB code: 2c0b was solved by M.J.Marcaida, A.J.Callaghan, W.G.Scott, B.F.Luisi, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	12.00 / 3.18
Space group	P 62 2 2
Cell size a, b, c (Å), α, β, γ (°)	196.049, 196.049, 142.660, 90.00, 90.00, 120.00
R / Rfree (%)	25.2 / 28.2

The structure of Catalytic Domain of E. Coli Rnase E in Complex with 13-Mer Rna also contains other interesting chemical elements:

Magnesium

(Mg)

2 atoms

The binding sites of Zinc atom in the Catalytic Domain of E. Coli Rnase E in Complex with 13-Mer Rna (pdb code 2c0b). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total only one binding site of Zinc was determined in the Catalytic Domain of E. Coli Rnase E in Complex with 13-Mer Rna, PDB code: 2c0b:

Zinc binding site 1 out of 1 in the Catalytic Domain of E. Coli Rnase E in Complex with 13-Mer Rna


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Catalytic Domain of E. Coli Rnase E in Complex with 13-Mer Rna within 5.0Å range: probe atom residue distance (Å) B Occ L:Zn1511 b:18.1 occ:0.50 SG L:CYS404 2.1 15.0 1.0 SG L:CYS407 2.1 15.2 1.0 CB L:CYS404 3.1 15.0 1.0 CB L:CYS407 3.3 29.4 1.0 N L:CYS407 3.5 37.1 1.0 O L:CYS407 4.0 44.7 1.0 CA L:CYS407 4.0 36.8 1.0 CB L:ARG406 4.1 15.0 1.0 C L:CYS407 4.3 30.8 1.0 CA L:CYS404 4.5 15.3 1.0 C L:ARG406 4.6 25.8 1.0 C L:CYS404 4.7 15.0 1.0 CA L:GLY411 4.7 15.0 1.0 CG L:ARG406 4.7 15.0 1.0 O L:CYS404 4.7 17.4 1.0 N L:ARG406 4.7 15.0 1.0 CA L:ARG406 4.7 15.0 1.0 N L:GLY411 4.9 16.4 1.0

A.J.Callaghan, M.J.Marcaida, J.A.Stead, K.J.Mcdowall, W.G.Scott, B.F.Luisi. Structure of E. Coli Rnase E Catalytic Domain and Implications For Rna Processing and Turnover Nature V. 437 1187 2005.
ISSN: ISSN 0028-0836
PubMed: 16237448
DOI: 10.1038/NATURE04084