Zinc in PDB 2axr: Crystal Structure of Glucooligosaccharide Oxidase From Acremonium Strictum: A Novel Flavinylation of 6-S-Cysteinyl, 8ALPHA-N1-Histidyl Fad

The structure of Crystal Structure of Glucooligosaccharide Oxidase From Acremonium Strictum: A Novel Flavinylation of 6-S-Cysteinyl, 8ALPHA-N1-Histidyl Fad, PDB code: 2axr was solved by C.-H.Huang, W.-L.Lai, A.Vasella, Y.-C.Tsai, S.-H.Liaw, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	50.00 / 1.98
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	53.686, 91.123, 112.288, 90.00, 90.00, 90.00
R / Rfree (%)	19.9 / 24

The binding sites of Zinc atom in the Crystal Structure of Glucooligosaccharide Oxidase From Acremonium Strictum: A Novel Flavinylation of 6-S-Cysteinyl, 8ALPHA-N1-Histidyl Fad (pdb code 2axr). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 4 binding sites of Zinc where determined in the Crystal Structure of Glucooligosaccharide Oxidase From Acremonium Strictum: A Novel Flavinylation of 6-S-Cysteinyl, 8ALPHA-N1-Histidyl Fad, PDB code: 2axr:
Jump to Zinc binding site number: 1; 2; 3; 4;

Zinc binding site 1 out of 4 in the Crystal Structure of Glucooligosaccharide Oxidase From Acremonium Strictum: A Novel Flavinylation of 6-S-Cysteinyl, 8ALPHA-N1-Histidyl Fad


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Crystal Structure of Glucooligosaccharide Oxidase From Acremonium Strictum: A Novel Flavinylation of 6-S-Cysteinyl, 8ALPHA-N1-Histidyl Fad within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn500 b:55.8 occ:1.00 OD2 A:ASP36 2.0 44.0 1.0 OE1 A:GLU17 2.3 45.5 1.0 OE2 A:GLU17 2.6 44.6 1.0 CD A:GLU17 2.8 42.5 1.0 CG A:ASP36 2.8 38.4 1.0 OD1 A:ASP36 3.0 40.1 1.0 O A:PRO37 3.9 24.3 1.0 CZ2 A:TRP22 4.1 24.7 1.0 CB A:ASP36 4.2 33.2 1.0 CG A:GLU17 4.3 39.6 1.0 CH2 A:TRP22 4.6 23.8 1.0 C A:ASP36 5.0 26.9 1.0

Zinc binding site 2 out of 4 in the Crystal Structure of Glucooligosaccharide Oxidase From Acremonium Strictum: A Novel Flavinylation of 6-S-Cysteinyl, 8ALPHA-N1-Histidyl Fad


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Crystal Structure of Glucooligosaccharide Oxidase From Acremonium Strictum: A Novel Flavinylation of 6-S-Cysteinyl, 8ALPHA-N1-Histidyl Fad within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn501 b:32.7 occ:1.00 OE1 A:GLU-4 1.9 31.1 1.0 OE2 A:GLU-2 1.9 32.2 1.0 OE1 A:GLU16 2.1 37.8 1.0 OE2 A:GLU16 2.2 37.3 1.0 CD A:GLU16 2.4 36.4 1.0 CD A:GLU-4 2.6 33.5 1.0 OE2 A:GLU-4 2.8 30.5 1.0 CD A:GLU-2 2.9 30.9 1.0 CG A:GLU-2 3.3 30.2 1.0 OD1 A:ASP18 3.9 29.4 1.0 CG A:GLU16 4.0 35.1 1.0 OE1 A:GLU-2 4.0 29.9 1.0 CG A:GLU-4 4.1 33.2 1.0 CB A:GLU-2 4.4 30.3 1.0 O A:GLU-4 4.5 37.7 1.0 CG A:ASP18 4.6 29.1 1.0 OD2 A:ASP18 4.6 30.9 1.0 CB A:GLU16 4.8 30.5 1.0

Zinc binding site 3 out of 4 in the Crystal Structure of Glucooligosaccharide Oxidase From Acremonium Strictum: A Novel Flavinylation of 6-S-Cysteinyl, 8ALPHA-N1-Histidyl Fad


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 3 of Crystal Structure of Glucooligosaccharide Oxidase From Acremonium Strictum: A Novel Flavinylation of 6-S-Cysteinyl, 8ALPHA-N1-Histidyl Fad within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn502 b:35.0 occ:1.00 OE2 A:GLU241 2.0 27.8 1.0 O A:HOH699 2.2 28.8 1.0 OE1 A:GLU241 2.6 24.3 1.0 CD A:GLU241 2.6 26.9 1.0 O A:HOH733 4.0 29.7 1.0 CG A:GLU241 4.1 24.7 1.0 CB A:PRO239 4.1 26.6 1.0 CG A:PRO239 4.9 27.1 1.0

Zinc binding site 4 out of 4 in the Crystal Structure of Glucooligosaccharide Oxidase From Acremonium Strictum: A Novel Flavinylation of 6-S-Cysteinyl, 8ALPHA-N1-Histidyl Fad


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 4 of Crystal Structure of Glucooligosaccharide Oxidase From Acremonium Strictum: A Novel Flavinylation of 6-S-Cysteinyl, 8ALPHA-N1-Histidyl Fad within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn503 b:47.7 occ:1.00 OE2 A:GLU329 2.2 34.8 1.0 OE1 A:GLU329 2.5 33.4 1.0 O A:HOH556 2.5 44.1 1.0 CD A:GLU329 2.7 32.1 1.0 CG A:GLU329 4.2 31.8 1.0 CG2 A:THR417 4.5 28.1 1.0 CA A:THR417 4.9 28.3 1.0 CB A:THR417 4.9 28.3 1.0 O A:HOH669 4.9 32.8 1.0

C.-H.Huang, W.-L.Lai, M.H.Lee, C.J.Chen, A.Vasella, Y.-C.Tsai, S.-H.Liaw. Crystal Structure of Glucooligosaccharide Oxidase From Acremonium Strictum: A Novel Flavinylation of 6-S-Cysteinyl, 8{Alpha}-N1-Histidyl Fad J.Biol.Chem. V. 280 38831 2005.
ISSN: ISSN 0021-9258
PubMed: 16154992
DOI: 10.1074/JBC.M506078200