Zinc in PDB 1zr6: The Crystal Structure of An Acremonium Strictum Glucooligosaccharide Oxidase Reveals A Novel Flavinylation

The structure of The Crystal Structure of An Acremonium Strictum Glucooligosaccharide Oxidase Reveals A Novel Flavinylation, PDB code: 1zr6 was solved by C.-H.Huang, W.-L.Lai, M.-H.Lee, Y.-C.Tsai, S.-H.Liaw, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	50.00 / 1.55
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	53.084, 90.907, 111.679, 90.00, 90.00, 90.00
R / Rfree (%)	18 / 20

The binding sites of Zinc atom in the The Crystal Structure of An Acremonium Strictum Glucooligosaccharide Oxidase Reveals A Novel Flavinylation (pdb code 1zr6). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 4 binding sites of Zinc where determined in the The Crystal Structure of An Acremonium Strictum Glucooligosaccharide Oxidase Reveals A Novel Flavinylation, PDB code: 1zr6:
Jump to Zinc binding site number: 1; 2; 3; 4;

Zinc binding site 1 out of 4 in the The Crystal Structure of An Acremonium Strictum Glucooligosaccharide Oxidase Reveals A Novel Flavinylation


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of The Crystal Structure of An Acremonium Strictum Glucooligosaccharide Oxidase Reveals A Novel Flavinylation within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn1481 b:20.8 occ:1.00 OE1 A:GLU17 2.1 21.6 1.0 OD1 A:ASP36 2.1 18.4 1.0 O A:HOH1540 2.3 22.8 1.0 OD2 A:ASP36 2.4 19.9 1.0 CG A:ASP36 2.5 19.7 1.0 CD A:GLU17 3.0 22.3 1.0 OE2 A:GLU17 3.2 22.5 1.0 CB A:ASP36 4.0 16.1 1.0 O A:PRO37 4.3 15.6 1.0 CG A:GLU17 4.3 20.4 1.0 CZ2 A:TRP22 4.5 13.7 1.0 O A:HOH1770 4.6 39.7 1.0 O A:HOH1611 4.9 28.2 1.0 CA A:ASP36 5.0 16.2 1.0

Zinc binding site 2 out of 4 in the The Crystal Structure of An Acremonium Strictum Glucooligosaccharide Oxidase Reveals A Novel Flavinylation


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of The Crystal Structure of An Acremonium Strictum Glucooligosaccharide Oxidase Reveals A Novel Flavinylation within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn1482 b:23.8 occ:1.00 OE2 A:GLU-2 2.0 20.9 1.0 OE1 A:GLU-4 2.2 28.6 1.0 OE2 A:GLU16 2.2 33.6 1.0 O A:HOH1952 2.3 26.1 1.0 O A:HOH1518 2.5 18.9 1.0 OE2 A:GLU-4 2.8 28.8 1.0 CD A:GLU-4 2.8 30.1 1.0 CD A:GLU16 2.9 30.0 1.0 OE1 A:GLU16 2.9 30.9 1.0 CD A:GLU-2 3.0 19.3 1.0 CG A:GLU-2 3.3 18.6 1.0 OD1 A:ASP18 3.9 23.1 1.0 OE1 A:GLU-2 4.1 19.6 1.0 CG A:GLU16 4.3 25.8 1.0 CG A:GLU-4 4.3 29.7 1.0 CB A:GLU-2 4.3 19.0 1.0 O A:GLU-4 4.4 26.5 1.0 OD2 A:ASP18 4.6 25.6 1.0 N A:GLU-4 4.6 28.8 1.0 CG A:ASP18 4.6 23.9 1.0 CB A:GLU16 4.8 21.4 1.0 O A:HOH1814 4.9 33.1 1.0

Zinc binding site 3 out of 4 in the The Crystal Structure of An Acremonium Strictum Glucooligosaccharide Oxidase Reveals A Novel Flavinylation


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 3 of The Crystal Structure of An Acremonium Strictum Glucooligosaccharide Oxidase Reveals A Novel Flavinylation within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn1483 b:30.3 occ:1.00 OE2 A:GLU241 2.1 25.2 1.0 O A:HOH1678 2.4 35.1 1.0 OE1 A:GLU241 2.4 19.2 1.0 CD A:GLU241 2.6 22.3 1.0 CG A:GLU241 4.0 18.9 1.0 CB A:PRO239 4.1 19.8 1.0 O A:HOH1852 4.2 35.1 1.0 O A:HOH1954 4.3 36.6 1.0 CG A:PRO239 5.0 22.9 1.0

Zinc binding site 4 out of 4 in the The Crystal Structure of An Acremonium Strictum Glucooligosaccharide Oxidase Reveals A Novel Flavinylation


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 4 of The Crystal Structure of An Acremonium Strictum Glucooligosaccharide Oxidase Reveals A Novel Flavinylation within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn1484 b:32.3 occ:1.00 OE2 A:GLU329 2.1 24.6 1.0 OE1 A:GLU329 2.4 23.4 1.0 O A:HOH1612 2.4 28.4 1.0 CD A:GLU329 2.5 23.0 1.0 CG A:GLU329 4.0 19.8 1.0 CG2 A:THR417 4.7 16.9 1.0 O A:HOH1560 4.8 22.7 1.0 CB A:GLU329 4.9 18.6 1.0

C.H.Huang, W.L.Lai, M.H.Lee, C.J.Chen, A.Vasella, Y.C.Tsai, S.H.Liaw. Crystal Structure of Glucooligosaccharide Oxidase From Acremonium Strictum: A Novel Flavinylation of 6-S-Cysteinyl, 8ALPHA-N1-Histidyl Fad J.Biol.Chem. V. 280 38831 2005.
ISSN: ISSN 0021-9258
PubMed: 16154992
DOI: 10.1074/JBC.M506078200