Zinc in PDB 1y7w: Crystal Structure of A Halotolerant Carbonic Anhydrase From Dunaliella Salina

All present enzymatic activity of Crystal Structure of A Halotolerant Carbonic Anhydrase From Dunaliella Salina:
4.2.1.1;

The structure of Crystal Structure of A Halotolerant Carbonic Anhydrase From Dunaliella Salina, PDB code: 1y7w was solved by L.Premkumar, H.M.Greenblatt, U.K.Bageshwar, T.Savchenko, I.Gokhman, J.L.Sussman, A.Zamir, Israel Structural Proteomics Center (Ispc), with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	32.95 / 1.86
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	47.022, 119.835, 58.442, 90.00, 94.55, 90.00
R / Rfree (%)	16.7 / 20.2

The structure of Crystal Structure of A Halotolerant Carbonic Anhydrase From Dunaliella Salina also contains other interesting chemical elements:

Sodium

(Na)

2 atoms

The binding sites of Zinc atom in the Crystal Structure of A Halotolerant Carbonic Anhydrase From Dunaliella Salina (pdb code 1y7w). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 3 binding sites of Zinc where determined in the Crystal Structure of A Halotolerant Carbonic Anhydrase From Dunaliella Salina, PDB code: 1y7w:
Jump to Zinc binding site number: 1; 2; 3;

Zinc binding site 1 out of 3 in the Crystal Structure of A Halotolerant Carbonic Anhydrase From Dunaliella Salina


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Crystal Structure of A Halotolerant Carbonic Anhydrase From Dunaliella Salina within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn283 b:17.7 occ:1.00 O A:HOH330 1.9 28.9 1.0 NE2 A:HIS115 2.0 15.2 1.0 ND1 A:HIS135 2.0 14.7 1.0 NE2 A:HIS117 2.1 15.8 1.0 O A:ACY279 2.6 40.4 1.0 CE1 A:HIS135 2.9 15.3 1.0 CD2 A:HIS117 2.9 15.2 1.0 CD2 A:HIS115 3.0 14.3 1.0 CE1 A:HIS115 3.0 16.8 1.0 CG A:HIS135 3.1 14.3 1.0 CE1 A:HIS117 3.1 18.9 1.0 CB A:HIS135 3.5 15.2 1.0 C A:ACY279 3.6 40.8 1.0 OG1 A:THR217 3.8 14.1 1.0 OXT A:ACY279 3.9 39.6 1.0 O A:HOH409 3.9 31.9 1.0 OE1 A:GLU122 4.0 14.1 1.0 NE2 A:HIS135 4.1 12.7 1.0 ND1 A:HIS115 4.1 14.9 1.0 CG A:HIS115 4.1 16.0 1.0 CG A:HIS117 4.1 14.0 1.0 ND1 A:HIS117 4.2 14.7 1.0 CD2 A:HIS135 4.2 13.8 1.0 O A:HOH476 4.4 53.4 1.0 O A:HOH457 4.7 37.6 1.0 CH3 A:ACY279 4.8 40.6 1.0 CD A:GLU122 4.8 16.0 1.0

Zinc binding site 2 out of 3 in the Crystal Structure of A Halotolerant Carbonic Anhydrase From Dunaliella Salina


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Crystal Structure of A Halotolerant Carbonic Anhydrase From Dunaliella Salina within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn284 b:18.0 occ:1.00 ND1 B:HIS135 2.0 12.5 1.0 NE2 B:HIS115 2.1 13.6 1.0 O B:HOH324 2.1 24.5 1.0 NE2 B:HIS117 2.1 14.5 1.0 O B:ACY280 2.3 38.8 1.0 CE1 B:HIS135 2.9 14.7 1.0 CD2 B:HIS115 3.0 15.8 1.0 CD2 B:HIS117 3.0 13.1 1.0 CG B:HIS135 3.1 12.8 1.0 CE1 B:HIS115 3.1 18.0 1.0 CE1 B:HIS117 3.2 16.7 1.0 C B:ACY280 3.4 40.3 1.0 CB B:HIS135 3.6 13.3 1.0 OG1 B:THR217 3.8 13.8 1.0 O B:HOH448 3.9 37.7 1.0 OXT B:ACY280 4.0 37.1 1.0 OE1 B:GLU122 4.0 15.3 1.0 NE2 B:HIS135 4.0 15.1 1.0 O B:HOH535 4.1 45.3 1.0 CG B:HIS115 4.1 16.4 1.0 CD2 B:HIS135 4.2 11.7 1.0 ND1 B:HIS115 4.2 13.5 1.0 CG B:HIS117 4.2 15.2 1.0 ND1 B:HIS117 4.3 14.1 1.0 CH3 B:ACY280 4.6 40.6 1.0 O B:HOH543 4.9 43.9 1.0 CD B:GLU122 5.0 17.2 1.0

Zinc binding site 3 out of 3 in the Crystal Structure of A Halotolerant Carbonic Anhydrase From Dunaliella Salina


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 3 of Crystal Structure of A Halotolerant Carbonic Anhydrase From Dunaliella Salina within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn285 b:19.8 occ:1.00 O A:HOH416 2.0 27.9 1.0 NE2 B:HIS13 2.1 17.9 1.0 O B:HOH327 2.1 26.8 1.0 NE2 A:HIS13 2.1 14.1 1.0 CE1 B:HIS13 3.0 16.9 1.0 CD2 A:HIS13 3.1 14.9 1.0 CD2 B:HIS13 3.1 16.1 1.0 CE1 A:HIS13 3.1 15.7 1.0 O A:HOH441 3.5 37.8 1.0 O B:HOH540 3.9 35.2 1.0 ND1 B:HIS13 4.1 15.1 1.0 ND1 A:HIS13 4.2 14.1 1.0 CG B:HIS13 4.2 16.0 1.0 O A:MET11 4.2 20.4 0.5 CG A:HIS13 4.2 15.0 1.0 O A:MET11 4.2 20.9 0.5 O B:HOH545 4.2 41.7 1.0 O B:MET11 4.3 17.9 1.0 O B:TYR10 4.5 15.2 1.0 O A:TYR10 4.7 16.5 1.0

L.Premkumar, H.M.Greenblatt, U.K.Bageshwar, T.Savchenko, I.Gokhman, J.L.Sussman, A.Zamir. Three-Dimensional Structure of A Halotolerant Algal Carbonic Anhydrase Predicts Halotolerance of A Mammalian Homolog. Proc.Natl.Acad.Sci.Usa V. 102 7493 2005.
ISSN: ISSN 0027-8424
PubMed: 15894606
DOI: 10.1073/PNAS.0502829102