Zinc in PDB 1ux0: Bacillus Subtilis Cytidine Deaminase with An ARG56 - Gln Substitution

All present enzymatic activity of Bacillus Subtilis Cytidine Deaminase with An ARG56 - Gln Substitution:
3.5.4.5;

The structure of Bacillus Subtilis Cytidine Deaminase with An ARG56 - Gln Substitution, PDB code: 1ux0 was solved by E.Johansson, J.Neuhard, M.Willemoes, S.Larsen, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	19.95 / 1.99
Space group	C 1 2 1
Cell size a, b, c (Å), α, β, γ (°)	74.880, 66.098, 55.514, 90.00, 115.64, 90.00
R / Rfree (%)	19.1 / 20.7

The binding sites of Zinc atom in the Bacillus Subtilis Cytidine Deaminase with An ARG56 - Gln Substitution (pdb code 1ux0). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Bacillus Subtilis Cytidine Deaminase with An ARG56 - Gln Substitution, PDB code: 1ux0:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Bacillus Subtilis Cytidine Deaminase with An ARG56 - Gln Substitution


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Bacillus Subtilis Cytidine Deaminase with An ARG56 - Gln Substitution within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn1131 b:28.4 occ:1.00 SG A:CYS86 2.3 21.1 1.0 O4 A:THU138 2.4 16.5 1.0 SG A:CYS53 2.4 24.5 1.0 SG A:CYS89 2.5 25.1 1.0 CB A:CYS53 3.0 19.2 1.0 CB A:CYS89 3.1 19.8 1.0 CB A:CYS86 3.4 17.7 1.0 C4 A:THU138 3.4 22.0 1.0 N3 A:THU138 3.6 21.9 1.0 N A:CYS86 3.7 16.9 1.0 C6 A:THU138 4.0 22.0 1.0 OE2 A:GLU55 4.0 19.6 1.0 N A:CYS89 4.0 17.1 1.0 C5 A:THU138 4.0 21.9 1.0 CA A:CYS86 4.1 17.6 1.0 CA A:CYS89 4.1 18.8 1.0 C2 A:THU138 4.1 22.7 1.0 O A:CYS86 4.4 16.9 1.0 OE1 A:GLU55 4.5 19.1 1.0 N1 A:THU138 4.5 23.5 1.0 CD A:GLU55 4.5 19.8 1.0 CA A:CYS53 4.5 17.6 1.0 C A:CYS86 4.5 17.4 1.0 O A:HOH2038 4.7 30.0 1.0 NE2 A:GLN56 4.8 31.0 1.0 C A:PRO85 4.8 16.7 1.0 O2 A:THU138 4.8 21.1 1.0

Zinc binding site 2 out of 2 in the Bacillus Subtilis Cytidine Deaminase with An ARG56 - Gln Substitution


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Bacillus Subtilis Cytidine Deaminase with An ARG56 - Gln Substitution within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn1131 b:35.9 occ:1.00 SG B:CYS86 2.3 21.7 1.0 SG B:CYS53 2.4 27.1 1.0 O4 B:THU138 2.4 21.9 1.0 SG B:CYS89 2.5 28.4 1.0 CB B:CYS53 3.0 20.4 1.0 CB B:CYS89 3.1 22.6 1.0 CB B:CYS86 3.4 16.4 1.0 C4 B:THU138 3.5 23.0 1.0 N3 B:THU138 3.7 23.3 1.0 N B:CYS86 3.7 16.1 1.0 N B:CYS89 4.0 18.3 1.0 C6 B:THU138 4.0 21.8 1.0 CA B:CYS86 4.1 16.8 1.0 C5 B:THU138 4.1 23.8 1.0 OE2 B:GLU55 4.1 22.6 1.0 CA B:CYS89 4.1 20.5 1.0 C2 B:THU138 4.2 22.2 1.0 O B:CYS86 4.4 18.6 1.0 N1 B:THU138 4.5 24.0 1.0 CA B:CYS53 4.5 18.3 1.0 C B:CYS86 4.5 16.8 1.0 OE1 B:GLU55 4.6 21.1 1.0 CD B:GLU55 4.6 22.0 1.0 NE2 B:GLN56 4.8 31.2 1.0 C B:PRO85 4.9 16.7 1.0 O2 B:THU138 4.9 20.7 1.0

E.Johansson, J.Neuhard, M.Willemoes, S.Larsen. Structural, Kinetic, and Mutational Studies of the Zinc Ion Environment in Tetrameric Cytidine Deaminase Biochemistry V. 43 6020 2004.
ISSN: ISSN 0006-2960
PubMed: 15147186
DOI: 10.1021/BI035893X