Zinc in PDB 1uwz: Bacillus Subtilis Cytidine Deaminase with An ARG56 - Ala Substitution

All present enzymatic activity of Bacillus Subtilis Cytidine Deaminase with An ARG56 - Ala Substitution:
3.5.4.5;

The structure of Bacillus Subtilis Cytidine Deaminase with An ARG56 - Ala Substitution, PDB code: 1uwz was solved by E.Johansson, J.Neuhard, M.Willemoes, S.Larsen, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	19.96 / 1.99
Space group	C 1 2 1
Cell size a, b, c (Å), α, β, γ (°)	73.743, 66.418, 55.388, 90.00, 115.60, 90.00
R / Rfree (%)	18.8 / 21.1

The binding sites of Zinc atom in the Bacillus Subtilis Cytidine Deaminase with An ARG56 - Ala Substitution (pdb code 1uwz). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Bacillus Subtilis Cytidine Deaminase with An ARG56 - Ala Substitution, PDB code: 1uwz:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Bacillus Subtilis Cytidine Deaminase with An ARG56 - Ala Substitution


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Bacillus Subtilis Cytidine Deaminase with An ARG56 - Ala Substitution within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn1131 b:29.0 occ:1.00 SG A:CYS86 2.3 17.4 1.0 O4 A:THU138 2.4 20.6 1.0 SG A:CYS53 2.4 24.6 1.0 SG A:CYS89 2.5 25.2 1.0 CB A:CYS53 3.0 19.9 1.0 CB A:CYS89 3.1 20.8 1.0 C4 A:THU138 3.4 23.4 1.0 CB A:CYS86 3.4 19.7 1.0 N3 A:THU138 3.6 24.9 1.0 N A:CYS86 3.8 17.6 1.0 C6 A:THU138 3.8 21.7 1.0 C5 A:THU138 3.9 23.0 1.0 N A:CYS89 4.0 17.9 1.0 OE2 A:GLU55 4.1 21.6 1.0 C2 A:THU138 4.1 23.8 1.0 CA A:CYS89 4.1 20.2 1.0 CA A:CYS86 4.1 18.0 1.0 O A:HOH2051 4.1 35.1 1.0 N1 A:THU138 4.3 24.7 1.0 O A:CYS86 4.4 17.4 1.0 CA A:CYS53 4.5 19.3 1.0 OE1 A:GLU55 4.5 21.6 1.0 CD A:GLU55 4.6 22.1 1.0 C A:CYS86 4.6 18.0 1.0 O2 A:THU138 4.8 21.2 1.0 C A:PRO85 4.9 17.8 1.0

Zinc binding site 2 out of 2 in the Bacillus Subtilis Cytidine Deaminase with An ARG56 - Ala Substitution


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Bacillus Subtilis Cytidine Deaminase with An ARG56 - Ala Substitution within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn1131 b:32.5 occ:1.00 SG B:CYS86 2.4 19.1 1.0 O4 B:THU138 2.4 20.5 1.0 SG B:CYS53 2.4 24.8 1.0 SG B:CYS89 2.5 26.9 1.0 CB B:CYS53 3.0 19.7 1.0 CB B:CYS89 3.1 21.2 1.0 C4 B:THU138 3.4 24.0 1.0 CB B:CYS86 3.4 18.4 1.0 N3 B:THU138 3.6 24.3 1.0 N B:CYS86 3.8 15.3 1.0 C6 B:THU138 3.9 23.5 1.0 C5 B:THU138 3.9 24.1 1.0 N B:CYS89 4.0 16.5 1.0 OE2 B:GLU55 4.1 24.3 1.0 C2 B:THU138 4.1 24.4 1.0 CA B:CYS89 4.1 19.6 1.0 CA B:CYS86 4.1 16.7 1.0 N1 B:THU138 4.4 25.4 1.0 O B:CYS86 4.5 17.4 1.0 OE1 B:GLU55 4.5 21.9 1.0 CA B:CYS53 4.5 18.9 1.0 CD B:GLU55 4.5 23.1 1.0 C B:CYS86 4.6 17.1 1.0 O B:HOH2031 4.7 42.3 1.0 O2 B:THU138 4.8 22.5 1.0 C B:PRO85 5.0 16.1 1.0

E.Johansson, J.Neuhard, M.Willemoes, S.Larsen. Structural, Kinetic, and Mutational Studies of the Zinc Ion Environment in Tetrameric Cytidine Deaminase Biochemistry V. 43 6020 2004.
ISSN: ISSN 0006-2960
PubMed: 15147186
DOI: 10.1021/BI035893X