Zinc in PDB 1uol: Crystal Structure of the Human P53 Core Domain Mutant M133L/V203A/N239Y/N268D at 1.9 A Resolution.

The structure of Crystal Structure of the Human P53 Core Domain Mutant M133L/V203A/N239Y/N268D at 1.9 A Resolution., PDB code: 1uol was solved by A.C.Joerger, M.D.Allen, A.R.Fersht, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	35.6 / 1.9
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	65.000, 71.000, 104.800, 90.00, 90.00, 90.00
R / Rfree (%)	19.2 / 23

The binding sites of Zinc atom in the Crystal Structure of the Human P53 Core Domain Mutant M133L/V203A/N239Y/N268D at 1.9 A Resolution. (pdb code 1uol). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Crystal Structure of the Human P53 Core Domain Mutant M133L/V203A/N239Y/N268D at 1.9 A Resolution., PDB code: 1uol:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Crystal Structure of the Human P53 Core Domain Mutant M133L/V203A/N239Y/N268D at 1.9 A Resolution.


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Crystal Structure of the Human P53 Core Domain Mutant M133L/V203A/N239Y/N268D at 1.9 A Resolution. within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn1300 b:11.2 occ:1.00 ND1 A:HIS179 2.0 5.0 1.0 SG A:CYS242 2.3 12.1 1.0 SG A:CYS238 2.3 11.6 1.0 SG A:CYS176 2.4 10.2 1.0 CE1 A:HIS179 3.0 10.2 1.0 CG A:HIS179 3.1 7.4 1.0 CB A:CYS242 3.1 13.5 1.0 CB A:CYS238 3.3 10.4 1.0 CB A:HIS179 3.4 8.2 1.0 CB A:CYS176 3.5 11.1 1.0 CA A:CYS238 3.8 8.6 1.0 O A:HOH2112 4.0 23.9 1.0 N A:CYS176 4.0 8.4 1.0 NE2 A:HIS179 4.1 8.1 1.0 CD2 A:HIS179 4.2 10.1 1.0 N A:HIS179 4.3 13.0 1.0 CA A:CYS176 4.3 10.2 1.0 N A:TYR239 4.4 7.5 1.0 CA A:HIS179 4.5 11.7 1.0 CA A:CYS242 4.6 14.6 1.0 O A:MET237 4.6 9.3 1.0 C A:CYS238 4.6 8.1 1.0 O A:HOH2099 4.7 13.8 1.0 N A:CYS238 5.0 8.2 1.0

Zinc binding site 2 out of 2 in the Crystal Structure of the Human P53 Core Domain Mutant M133L/V203A/N239Y/N268D at 1.9 A Resolution.


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Crystal Structure of the Human P53 Core Domain Mutant M133L/V203A/N239Y/N268D at 1.9 A Resolution. within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn1300 b:12.5 occ:1.00 ND1 B:HIS179 2.0 5.7 1.0 SG B:CYS242 2.3 11.3 1.0 SG B:CYS176 2.4 10.6 1.0 SG B:CYS238 2.4 10.7 1.0 CE1 B:HIS179 3.0 11.7 1.0 CG B:HIS179 3.0 7.8 1.0 CB B:CYS242 3.1 10.3 1.0 CB B:CYS238 3.4 11.6 1.0 CB B:HIS179 3.4 5.0 1.0 CB B:CYS176 3.5 8.8 1.0 CA B:CYS238 3.8 10.6 1.0 O B:HOH2105 3.9 30.0 1.0 N B:CYS176 4.1 9.0 1.0 NE2 B:HIS179 4.1 8.5 1.0 CD2 B:HIS179 4.1 9.6 1.0 N B:HIS179 4.3 11.8 1.0 CA B:CYS176 4.4 9.9 1.0 N B:TYR239 4.4 9.3 1.0 CA B:HIS179 4.5 9.6 1.0 CA B:CYS242 4.5 14.4 1.0 O B:HOH2095 4.6 14.1 1.0 O B:MET237 4.6 10.1 1.0 C B:CYS238 4.6 8.7 1.0 N B:CYS238 5.0 10.8 1.0

A.C.Joerger, M.D.Allen, A.R.Fersht. Crystal Structure of A Superstable Mutant of Human P53 Core Domain. Insights Into the Mechanism of Rescuing Oncogenic Mutations J.Biol.Chem. V. 279 1291 2004.
ISSN: ISSN 0021-9258
PubMed: 14534297
DOI: 10.1074/JBC.M309732200