Zinc in PDB 1twf: Rna Polymerase II Complexed with Utp at 2.3 A Resolution
Enzymatic activity of Rna Polymerase II Complexed with Utp at 2.3 A Resolution
All present enzymatic activity of Rna Polymerase II Complexed with Utp at 2.3 A Resolution:
2.7.7.6;
Protein crystallography data
The structure of Rna Polymerase II Complexed with Utp at 2.3 A Resolution, PDB code: 1twf
was solved by
K.D.Westover,
D.A.Bushnell,
R.D.Kornberg,
with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:
Resolution Low / High (Å)
|
40.00 /
2.30
|
Space group
|
I 2 2 2
|
Cell size a, b, c (Å), α, β, γ (°)
|
123.000,
223.000,
374.000,
90.00,
90.00,
90.00
|
R / Rfree (%)
|
24.7 /
29.4
|
Other elements in 1twf:
The structure of Rna Polymerase II Complexed with Utp at 2.3 A Resolution also contains other interesting chemical elements:
Zinc Binding Sites:
The binding sites of Zinc atom in the Rna Polymerase II Complexed with Utp at 2.3 A Resolution
(pdb code 1twf). This binding sites where shown within
5.0 Angstroms radius around Zinc atom.
In total 8 binding sites of Zinc where determined in the
Rna Polymerase II Complexed with Utp at 2.3 A Resolution, PDB code: 1twf:
Jump to Zinc binding site number:
1;
2;
3;
4;
5;
6;
7;
8;
Zinc binding site 1 out
of 8 in 1twf
Go back to
Zinc Binding Sites List in 1twf
Zinc binding site 1 out
of 8 in the Rna Polymerase II Complexed with Utp at 2.3 A Resolution
Mono view
Stereo pair view
|
A full contact list of Zinc with other atoms in the Zn binding
site number 1 of Rna Polymerase II Complexed with Utp at 2.3 A Resolution within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
J:Zn3001
b:45.9
occ:1.00
|
SG
|
J:CYS10
|
2.3
|
43.1
|
1.0
|
SG
|
J:CYS7
|
2.4
|
33.4
|
1.0
|
SG
|
J:CYS45
|
2.5
|
37.3
|
1.0
|
SG
|
J:CYS46
|
2.5
|
46.9
|
1.0
|
N
|
J:CYS46
|
3.1
|
41.8
|
1.0
|
CB
|
J:CYS45
|
3.4
|
39.8
|
1.0
|
CB
|
J:CYS7
|
3.5
|
36.2
|
1.0
|
CA
|
J:CYS46
|
3.5
|
41.8
|
1.0
|
C
|
J:CYS45
|
3.5
|
40.4
|
1.0
|
CB
|
J:CYS46
|
3.6
|
45.1
|
1.0
|
CB
|
J:CYS10
|
3.7
|
39.9
|
1.0
|
N
|
J:CYS10
|
3.9
|
39.7
|
1.0
|
NE
|
J:ARG43
|
3.9
|
37.4
|
1.0
|
CA
|
J:CYS45
|
4.0
|
40.1
|
1.0
|
O
|
J:CYS45
|
4.1
|
42.0
|
1.0
|
CD
|
J:ARG43
|
4.2
|
39.1
|
1.0
|
CG
|
J:ARG43
|
4.4
|
42.4
|
1.0
|
CA
|
J:CYS10
|
4.4
|
40.7
|
1.0
|
CB
|
J:SER9
|
4.4
|
35.8
|
1.0
|
N
|
J:CYS45
|
4.8
|
39.4
|
1.0
|
C
|
J:SER9
|
4.9
|
37.5
|
1.0
|
O
|
J:ARG43
|
4.9
|
37.5
|
1.0
|
CA
|
J:CYS7
|
4.9
|
34.7
|
1.0
|
CA
|
J:SER9
|
5.0
|
34.9
|
1.0
|
|
Zinc binding site 2 out
of 8 in 1twf
Go back to
Zinc Binding Sites List in 1twf
Zinc binding site 2 out
of 8 in the Rna Polymerase II Complexed with Utp at 2.3 A Resolution
Mono view
Stereo pair view
|
A full contact list of Zinc with other atoms in the Zn binding
site number 2 of Rna Polymerase II Complexed with Utp at 2.3 A Resolution within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
C:Zn3002
b:48.6
occ:1.00
|
SG
|
C:CYS86
|
2.4
|
48.3
|
1.0
|
SG
|
C:CYS88
|
2.4
|
55.2
|
1.0
|
SG
|
C:CYS95
|
2.4
|
52.4
|
1.0
|
SG
|
C:CYS92
|
2.7
|
56.1
|
1.0
|
CB
|
C:CYS86
|
3.3
|
48.5
|
1.0
|
N
|
C:CYS92
|
3.4
|
59.9
|
1.0
|
CB
|
C:CYS88
|
3.4
|
56.5
|
1.0
|
CB
|
C:CYS95
|
3.5
|
57.3
|
1.0
|
CB
|
C:CYS92
|
3.6
|
56.9
|
1.0
|
C
|
C:HIS91
|
3.8
|
60.9
|
1.0
|
CA
|
C:HIS91
|
4.0
|
62.3
|
1.0
|
CA
|
C:CYS92
|
4.0
|
58.3
|
1.0
|
N
|
C:CYS95
|
4.0
|
59.1
|
1.0
|
O
|
C:CYS92
|
4.3
|
58.1
|
1.0
|
CA
|
C:CYS95
|
4.4
|
57.5
|
1.0
|
C
|
C:CYS92
|
4.4
|
58.5
|
1.0
|
N
|
C:CYS88
|
4.5
|
53.2
|
1.0
|
CA
|
C:CYS88
|
4.5
|
55.8
|
1.0
|
O
|
C:ASP90
|
4.5
|
61.0
|
1.0
|
O
|
C:HIS91
|
4.6
|
60.5
|
1.0
|
CA
|
C:CYS86
|
4.6
|
50.2
|
1.0
|
CB
|
C:LYS94
|
4.7
|
61.5
|
1.0
|
N
|
C:HIS91
|
4.7
|
62.7
|
1.0
|
C
|
C:LYS94
|
4.8
|
59.9
|
1.0
|
C
|
C:ASP90
|
4.9
|
62.1
|
1.0
|
C
|
C:CYS86
|
4.9
|
51.1
|
1.0
|
|
Zinc binding site 3 out
of 8 in 1twf
Go back to
Zinc Binding Sites List in 1twf
Zinc binding site 3 out
of 8 in the Rna Polymerase II Complexed with Utp at 2.3 A Resolution
Mono view
Stereo pair view
|
A full contact list of Zinc with other atoms in the Zn binding
site number 3 of Rna Polymerase II Complexed with Utp at 2.3 A Resolution within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
I:Zn3003
b:58.0
occ:1.00
|
SG
|
I:CYS10
|
2.3
|
57.9
|
1.0
|
SG
|
I:CYS32
|
2.4
|
66.9
|
1.0
|
SG
|
I:CYS29
|
2.5
|
55.2
|
1.0
|
SG
|
I:CYS7
|
2.5
|
53.1
|
1.0
|
CB
|
I:CYS32
|
3.0
|
65.0
|
1.0
|
N
|
I:CYS32
|
3.3
|
66.0
|
1.0
|
CB
|
I:CYS29
|
3.4
|
60.5
|
1.0
|
CB
|
I:CYS7
|
3.6
|
57.8
|
1.0
|
CB
|
I:CYS10
|
3.7
|
57.0
|
1.0
|
CA
|
I:CYS32
|
3.7
|
64.4
|
1.0
|
CB
|
I:THR31
|
3.9
|
66.7
|
1.0
|
N
|
I:CYS10
|
4.1
|
58.2
|
1.0
|
CG2
|
I:THR31
|
4.3
|
67.1
|
1.0
|
C
|
I:THR31
|
4.4
|
66.2
|
1.0
|
C
|
I:CYS32
|
4.5
|
63.4
|
1.0
|
CA
|
I:CYS10
|
4.5
|
56.4
|
1.0
|
CB
|
I:TYR34
|
4.5
|
48.3
|
1.0
|
CA
|
I:THR31
|
4.6
|
66.2
|
1.0
|
N
|
I:THR31
|
4.7
|
66.1
|
1.0
|
CA
|
I:CYS29
|
4.9
|
60.5
|
1.0
|
OG1
|
I:THR31
|
4.9
|
65.4
|
1.0
|
O
|
I:CYS32
|
4.9
|
63.7
|
1.0
|
CB
|
I:ASP9
|
5.0
|
61.4
|
1.0
|
|
Zinc binding site 4 out
of 8 in 1twf
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Zinc Binding Sites List in 1twf
Zinc binding site 4 out
of 8 in the Rna Polymerase II Complexed with Utp at 2.3 A Resolution
Mono view
Stereo pair view
|
A full contact list of Zinc with other atoms in the Zn binding
site number 4 of Rna Polymerase II Complexed with Utp at 2.3 A Resolution within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
I:Zn3004
b:74.9
occ:1.00
|
SG
|
I:CYS106
|
2.4
|
70.6
|
1.0
|
SG
|
I:CYS78
|
2.6
|
89.5
|
1.0
|
SG
|
I:CYS75
|
2.6
|
87.7
|
1.0
|
SG
|
I:CYS103
|
2.7
|
65.9
|
1.0
|
CB
|
I:CYS103
|
3.0
|
59.2
|
1.0
|
CB
|
I:CYS75
|
3.4
|
89.0
|
1.0
|
N
|
I:CYS78
|
3.5
|
95.5
|
1.0
|
CB
|
I:CYS78
|
3.7
|
94.4
|
1.0
|
CB
|
I:HIS108
|
3.9
|
64.6
|
1.0
|
CB
|
I:LYS77
|
3.9
|
95.8
|
1.0
|
CB
|
I:CYS106
|
4.0
|
70.0
|
1.0
|
CA
|
I:CYS78
|
4.2
|
94.9
|
1.0
|
N
|
I:CYS106
|
4.5
|
69.0
|
1.0
|
CA
|
I:CYS103
|
4.5
|
58.1
|
1.0
|
OG
|
I:SER80
|
4.5
|
0.6
|
1.0
|
C
|
I:LYS77
|
4.5
|
95.5
|
1.0
|
CA
|
I:LYS77
|
4.6
|
95.3
|
1.0
|
N
|
I:HIS108
|
4.6
|
65.3
|
1.0
|
CG
|
I:HIS108
|
4.6
|
65.7
|
1.0
|
N
|
I:LYS77
|
4.7
|
94.3
|
1.0
|
CD2
|
I:HIS108
|
4.7
|
64.9
|
1.0
|
CA
|
I:CYS75
|
4.7
|
88.9
|
1.0
|
N
|
I:HIS79
|
4.7
|
96.3
|
1.0
|
CA
|
I:CYS106
|
4.8
|
69.2
|
1.0
|
C
|
I:CYS78
|
4.8
|
95.4
|
1.0
|
CA
|
I:HIS108
|
4.8
|
64.1
|
1.0
|
C
|
I:CYS75
|
5.0
|
89.8
|
1.0
|
O
|
I:CYS75
|
5.0
|
89.1
|
1.0
|
|
Zinc binding site 5 out
of 8 in 1twf
Go back to
Zinc Binding Sites List in 1twf
Zinc binding site 5 out
of 8 in the Rna Polymerase II Complexed with Utp at 2.3 A Resolution
Mono view
Stereo pair view
|
A full contact list of Zinc with other atoms in the Zn binding
site number 5 of Rna Polymerase II Complexed with Utp at 2.3 A Resolution within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
L:Zn3005
b:89.9
occ:1.00
|
SG
|
L:CYS34
|
2.6
|
94.4
|
1.0
|
SG
|
L:CYS31
|
2.8
|
0.0
|
1.0
|
SG
|
L:CYS48
|
2.8
|
0.4
|
1.0
|
CB
|
L:CYS51
|
2.8
|
0.3
|
1.0
|
CB
|
L:CYS48
|
3.0
|
0.9
|
1.0
|
SG
|
L:CYS51
|
3.3
|
0.0
|
1.0
|
O
|
L:CYS51
|
3.6
|
0.2
|
1.0
|
CB
|
L:CYS31
|
3.7
|
0.3
|
1.0
|
CA
|
L:CYS51
|
3.8
|
0.8
|
1.0
|
O
|
L:LYS49
|
3.8
|
0.6
|
1.0
|
N
|
L:CYS51
|
4.0
|
0.3
|
1.0
|
CB
|
L:CYS34
|
4.1
|
99.9
|
1.0
|
OG
|
L:SER36
|
4.1
|
1.0
|
1.0
|
C
|
L:CYS51
|
4.1
|
1.0
|
1.0
|
CA
|
L:CYS48
|
4.3
|
0.3
|
1.0
|
N
|
L:LYS49
|
4.5
|
0.3
|
1.0
|
C
|
L:CYS48
|
4.5
|
0.7
|
1.0
|
CD2
|
L:HIS53
|
4.6
|
0.6
|
1.0
|
C
|
L:LYS49
|
4.7
|
0.6
|
1.0
|
N
|
L:CYS34
|
4.7
|
0.1
|
1.0
|
CA
|
L:CYS34
|
5.0
|
0.2
|
1.0
|
|
Zinc binding site 6 out
of 8 in 1twf
Go back to
Zinc Binding Sites List in 1twf
Zinc binding site 6 out
of 8 in the Rna Polymerase II Complexed with Utp at 2.3 A Resolution
Mono view
Stereo pair view
|
A full contact list of Zinc with other atoms in the Zn binding
site number 6 of Rna Polymerase II Complexed with Utp at 2.3 A Resolution within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Zn3006
b:53.9
occ:1.00
|
SG
|
A:CYS110
|
2.4
|
54.5
|
1.0
|
SG
|
A:CYS107
|
2.5
|
66.8
|
1.0
|
SG
|
A:CYS148
|
2.5
|
80.6
|
1.0
|
SG
|
A:CYS167
|
2.6
|
82.8
|
1.0
|
CB
|
A:CYS167
|
3.0
|
85.9
|
1.0
|
CB
|
A:CYS107
|
3.3
|
65.7
|
1.0
|
CB
|
A:CYS148
|
3.3
|
86.9
|
1.0
|
CB
|
A:CYS110
|
4.0
|
64.6
|
1.0
|
CA
|
A:CYS167
|
4.2
|
87.1
|
1.0
|
N
|
A:CYS110
|
4.2
|
69.5
|
1.0
|
C
|
A:CYS167
|
4.2
|
87.0
|
1.0
|
O
|
A:CYS167
|
4.4
|
86.7
|
1.0
|
CA
|
A:CYS110
|
4.7
|
66.4
|
1.0
|
N
|
A:GLY168
|
4.7
|
85.7
|
1.0
|
CA
|
A:CYS107
|
4.7
|
65.9
|
1.0
|
CB
|
A:HIS109
|
4.8
|
76.6
|
1.0
|
CA
|
A:CYS148
|
4.8
|
89.2
|
1.0
|
N
|
A:CYS167
|
5.0
|
89.2
|
1.0
|
|
Zinc binding site 7 out
of 8 in 1twf
Go back to
Zinc Binding Sites List in 1twf
Zinc binding site 7 out
of 8 in the Rna Polymerase II Complexed with Utp at 2.3 A Resolution
Mono view
Stereo pair view
|
A full contact list of Zinc with other atoms in the Zn binding
site number 7 of Rna Polymerase II Complexed with Utp at 2.3 A Resolution within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
B:Zn3007
b:55.4
occ:1.00
|
SG
|
B:CYS1166
|
2.6
|
72.1
|
1.0
|
SG
|
B:CYS1185
|
2.7
|
100.0
|
1.0
|
SG
|
B:CYS1182
|
2.8
|
0.9
|
1.0
|
SG
|
B:CYS1163
|
2.8
|
68.0
|
1.0
|
CB
|
B:CYS1163
|
3.1
|
65.8
|
1.0
|
CB
|
B:CYS1185
|
3.3
|
0.8
|
1.0
|
CB
|
B:CYS1166
|
3.6
|
67.8
|
1.0
|
CB
|
B:CYS1182
|
3.9
|
0.8
|
1.0
|
N
|
B:CYS1166
|
4.0
|
67.0
|
1.0
|
OG1
|
B:THR1170
|
4.3
|
69.7
|
1.0
|
CA
|
B:CYS1166
|
4.4
|
68.0
|
1.0
|
O
|
B:LYS1183
|
4.5
|
0.2
|
1.0
|
O
|
B:CYS1166
|
4.6
|
68.4
|
1.0
|
CA
|
B:CYS1163
|
4.7
|
65.9
|
1.0
|
CB
|
B:ILE1165
|
4.7
|
68.6
|
1.0
|
CA
|
B:CYS1185
|
4.7
|
0.6
|
1.0
|
O
|
B:CYS1182
|
4.7
|
1.0
|
1.0
|
N
|
B:CYS1185
|
4.9
|
0.9
|
1.0
|
C
|
B:CYS1166
|
5.0
|
68.5
|
1.0
|
|
Zinc binding site 8 out
of 8 in 1twf
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Zinc Binding Sites List in 1twf
Zinc binding site 8 out
of 8 in the Rna Polymerase II Complexed with Utp at 2.3 A Resolution
Mono view
Stereo pair view
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A full contact list of Zinc with other atoms in the Zn binding
site number 8 of Rna Polymerase II Complexed with Utp at 2.3 A Resolution within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Zn3008
b:97.1
occ:1.00
|
SG
|
A:CYS77
|
2.6
|
0.5
|
1.0
|
NE2
|
A:HIS80
|
2.6
|
96.5
|
1.0
|
SG
|
A:CYS70
|
2.7
|
0.4
|
1.0
|
SG
|
A:CYS67
|
2.8
|
0.8
|
1.0
|
CD2
|
A:HIS80
|
3.3
|
95.7
|
1.0
|
CE1
|
A:HIS80
|
3.5
|
96.0
|
1.0
|
CB
|
A:CYS67
|
3.6
|
0.3
|
1.0
|
O
|
A:CYS67
|
3.8
|
0.2
|
1.0
|
CB
|
A:CYS77
|
4.0
|
0.7
|
1.0
|
CB
|
A:CYS70
|
4.2
|
0.7
|
1.0
|
CG
|
A:HIS80
|
4.3
|
95.5
|
1.0
|
ND1
|
A:HIS80
|
4.4
|
95.1
|
1.0
|
OE1
|
A:GLU72
|
4.6
|
0.1
|
1.0
|
C
|
A:CYS67
|
4.6
|
0.2
|
1.0
|
O
|
A:PRO78
|
4.7
|
0.9
|
1.0
|
CA
|
A:CYS67
|
4.8
|
0.4
|
1.0
|
CA
|
A:CYS77
|
4.8
|
0.7
|
1.0
|
CD
|
A:PRO78
|
4.9
|
1.0
|
1.0
|
N
|
A:PRO78
|
4.9
|
0.5
|
1.0
|
O
|
A:GLN71
|
4.9
|
0.3
|
1.0
|
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Reference:
K.D.Westover,
D.A.Bushnell,
R.D.Kornberg.
Structural Basis of Transcription: Nucleotide Selection By Rotation in the Rna Polymerase II Active Center. Cell(Cambridge,Mass.) V. 119 481 2004.
ISSN: ISSN 0092-8674
PubMed: 15537538
DOI: 10.1016/J.CELL.2004.10.016
Page generated: Wed Oct 16 19:19:04 2024
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