Atomistry » Zinc » PDB 1to5-1u22 » 1twf
Atomistry »
  Zinc »
    PDB 1to5-1u22 »
      1twf »

Zinc in PDB 1twf: Rna Polymerase II Complexed with Utp at 2.3 A Resolution

Enzymatic activity of Rna Polymerase II Complexed with Utp at 2.3 A Resolution

All present enzymatic activity of Rna Polymerase II Complexed with Utp at 2.3 A Resolution:
2.7.7.6;

Protein crystallography data

The structure of Rna Polymerase II Complexed with Utp at 2.3 A Resolution, PDB code: 1twf was solved by K.D.Westover, D.A.Bushnell, R.D.Kornberg, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 40.00 / 2.30
Space group I 2 2 2
Cell size a, b, c (Å), α, β, γ (°) 123.000, 223.000, 374.000, 90.00, 90.00, 90.00
R / Rfree (%) 24.7 / 29.4

Other elements in 1twf:

The structure of Rna Polymerase II Complexed with Utp at 2.3 A Resolution also contains other interesting chemical elements:

Manganese (Mn) 2 atoms

Zinc Binding Sites:

The binding sites of Zinc atom in the Rna Polymerase II Complexed with Utp at 2.3 A Resolution (pdb code 1twf). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 8 binding sites of Zinc where determined in the Rna Polymerase II Complexed with Utp at 2.3 A Resolution, PDB code: 1twf:
Jump to Zinc binding site number: 1; 2; 3; 4; 5; 6; 7; 8;

Zinc binding site 1 out of 8 in 1twf

Go back to Zinc Binding Sites List in 1twf
Zinc binding site 1 out of 8 in the Rna Polymerase II Complexed with Utp at 2.3 A Resolution


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Rna Polymerase II Complexed with Utp at 2.3 A Resolution within 5.0Å range:
probe atom residue distance (Å) B Occ
J:Zn3001

b:45.9
occ:1.00
 SG J:CYS10 2.3 43.1 1.0
SG J:CYS7 2.4 33.4 1.0
SG J:CYS45 2.5 37.3 1.0
SG J:CYS46 2.5 46.9 1.0
N J:CYS46 3.1 41.8 1.0
CB J:CYS45 3.4 39.8 1.0
CB J:CYS7 3.5 36.2 1.0
CA J:CYS46 3.5 41.8 1.0
C J:CYS45 3.5 40.4 1.0
CB J:CYS46 3.6 45.1 1.0
CB J:CYS10 3.7 39.9 1.0
N J:CYS10 3.9 39.7 1.0
NE J:ARG43 3.9 37.4 1.0
CA J:CYS45 4.0 40.1 1.0
O J:CYS45 4.1 42.0 1.0
CD J:ARG43 4.2 39.1 1.0
CG J:ARG43 4.4 42.4 1.0
CA J:CYS10 4.4 40.7 1.0
CB J:SER9 4.4 35.8 1.0
N J:CYS45 4.8 39.4 1.0
C J:SER9 4.9 37.5 1.0
O J:ARG43 4.9 37.5 1.0
CA J:CYS7 4.9 34.7 1.0
CA J:SER9 5.0 34.9 1.0

Zinc binding site 2 out of 8 in 1twf

Go back to Zinc Binding Sites List in 1twf
Zinc binding site 2 out of 8 in the Rna Polymerase II Complexed with Utp at 2.3 A Resolution


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Rna Polymerase II Complexed with Utp at 2.3 A Resolution within 5.0Å range:
probe atom residue distance (Å) B Occ
C:Zn3002

b:48.6
occ:1.00
 SG C:CYS86 2.4 48.3 1.0
SG C:CYS88 2.4 55.2 1.0
SG C:CYS95 2.4 52.4 1.0
SG C:CYS92 2.7 56.1 1.0
CB C:CYS86 3.3 48.5 1.0
N C:CYS92 3.4 59.9 1.0
CB C:CYS88 3.4 56.5 1.0
CB C:CYS95 3.5 57.3 1.0
CB C:CYS92 3.6 56.9 1.0
C C:HIS91 3.8 60.9 1.0
CA C:HIS91 4.0 62.3 1.0
CA C:CYS92 4.0 58.3 1.0
N C:CYS95 4.0 59.1 1.0
O C:CYS92 4.3 58.1 1.0
CA C:CYS95 4.4 57.5 1.0
C C:CYS92 4.4 58.5 1.0
N C:CYS88 4.5 53.2 1.0
CA C:CYS88 4.5 55.8 1.0
O C:ASP90 4.5 61.0 1.0
O C:HIS91 4.6 60.5 1.0
CA C:CYS86 4.6 50.2 1.0
CB C:LYS94 4.7 61.5 1.0
N C:HIS91 4.7 62.7 1.0
C C:LYS94 4.8 59.9 1.0
C C:ASP90 4.9 62.1 1.0
C C:CYS86 4.9 51.1 1.0

Zinc binding site 3 out of 8 in 1twf

Go back to Zinc Binding Sites List in 1twf
Zinc binding site 3 out of 8 in the Rna Polymerase II Complexed with Utp at 2.3 A Resolution


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 3 of Rna Polymerase II Complexed with Utp at 2.3 A Resolution within 5.0Å range:
probe atom residue distance (Å) B Occ
I:Zn3003

b:58.0
occ:1.00
 SG I:CYS10 2.3 57.9 1.0
SG I:CYS32 2.4 66.9 1.0
SG I:CYS29 2.5 55.2 1.0
SG I:CYS7 2.5 53.1 1.0
CB I:CYS32 3.0 65.0 1.0
N I:CYS32 3.3 66.0 1.0
CB I:CYS29 3.4 60.5 1.0
CB I:CYS7 3.6 57.8 1.0
CB I:CYS10 3.7 57.0 1.0
CA I:CYS32 3.7 64.4 1.0
CB I:THR31 3.9 66.7 1.0
N I:CYS10 4.1 58.2 1.0
CG2 I:THR31 4.3 67.1 1.0
C I:THR31 4.4 66.2 1.0
C I:CYS32 4.5 63.4 1.0
CA I:CYS10 4.5 56.4 1.0
CB I:TYR34 4.5 48.3 1.0
CA I:THR31 4.6 66.2 1.0
N I:THR31 4.7 66.1 1.0
CA I:CYS29 4.9 60.5 1.0
OG1 I:THR31 4.9 65.4 1.0
O I:CYS32 4.9 63.7 1.0
CB I:ASP9 5.0 61.4 1.0

Zinc binding site 4 out of 8 in 1twf

Go back to Zinc Binding Sites List in 1twf
Zinc binding site 4 out of 8 in the Rna Polymerase II Complexed with Utp at 2.3 A Resolution


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 4 of Rna Polymerase II Complexed with Utp at 2.3 A Resolution within 5.0Å range:
probe atom residue distance (Å) B Occ
I:Zn3004

b:74.9
occ:1.00
 SG I:CYS106 2.4 70.6 1.0
SG I:CYS78 2.6 89.5 1.0
SG I:CYS75 2.6 87.7 1.0
SG I:CYS103 2.7 65.9 1.0
CB I:CYS103 3.0 59.2 1.0
CB I:CYS75 3.4 89.0 1.0
N I:CYS78 3.5 95.5 1.0
CB I:CYS78 3.7 94.4 1.0
CB I:HIS108 3.9 64.6 1.0
CB I:LYS77 3.9 95.8 1.0
CB I:CYS106 4.0 70.0 1.0
CA I:CYS78 4.2 94.9 1.0
N I:CYS106 4.5 69.0 1.0
CA I:CYS103 4.5 58.1 1.0
OG I:SER80 4.5 0.6 1.0
C I:LYS77 4.5 95.5 1.0
CA I:LYS77 4.6 95.3 1.0
N I:HIS108 4.6 65.3 1.0
CG I:HIS108 4.6 65.7 1.0
N I:LYS77 4.7 94.3 1.0
CD2 I:HIS108 4.7 64.9 1.0
CA I:CYS75 4.7 88.9 1.0
N I:HIS79 4.7 96.3 1.0
CA I:CYS106 4.8 69.2 1.0
C I:CYS78 4.8 95.4 1.0
CA I:HIS108 4.8 64.1 1.0
C I:CYS75 5.0 89.8 1.0
O I:CYS75 5.0 89.1 1.0

Zinc binding site 5 out of 8 in 1twf

Go back to Zinc Binding Sites List in 1twf
Zinc binding site 5 out of 8 in the Rna Polymerase II Complexed with Utp at 2.3 A Resolution


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 5 of Rna Polymerase II Complexed with Utp at 2.3 A Resolution within 5.0Å range:
probe atom residue distance (Å) B Occ
L:Zn3005

b:89.9
occ:1.00
 SG L:CYS34 2.6 94.4 1.0
SG L:CYS31 2.8 0.0 1.0
SG L:CYS48 2.8 0.4 1.0
CB L:CYS51 2.8 0.3 1.0
CB L:CYS48 3.0 0.9 1.0
SG L:CYS51 3.3 0.0 1.0
O L:CYS51 3.6 0.2 1.0
CB L:CYS31 3.7 0.3 1.0
CA L:CYS51 3.8 0.8 1.0
O L:LYS49 3.8 0.6 1.0
N L:CYS51 4.0 0.3 1.0
CB L:CYS34 4.1 99.9 1.0
OG L:SER36 4.1 1.0 1.0
C L:CYS51 4.1 1.0 1.0
CA L:CYS48 4.3 0.3 1.0
N L:LYS49 4.5 0.3 1.0
C L:CYS48 4.5 0.7 1.0
CD2 L:HIS53 4.6 0.6 1.0
C L:LYS49 4.7 0.6 1.0
N L:CYS34 4.7 0.1 1.0
CA L:CYS34 5.0 0.2 1.0

Zinc binding site 6 out of 8 in 1twf

Go back to Zinc Binding Sites List in 1twf
Zinc binding site 6 out of 8 in the Rna Polymerase II Complexed with Utp at 2.3 A Resolution


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 6 of Rna Polymerase II Complexed with Utp at 2.3 A Resolution within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Zn3006

b:53.9
occ:1.00
 SG A:CYS110 2.4 54.5 1.0
SG A:CYS107 2.5 66.8 1.0
SG A:CYS148 2.5 80.6 1.0
SG A:CYS167 2.6 82.8 1.0
CB A:CYS167 3.0 85.9 1.0
CB A:CYS107 3.3 65.7 1.0
CB A:CYS148 3.3 86.9 1.0
CB A:CYS110 4.0 64.6 1.0
CA A:CYS167 4.2 87.1 1.0
N A:CYS110 4.2 69.5 1.0
C A:CYS167 4.2 87.0 1.0
O A:CYS167 4.4 86.7 1.0
CA A:CYS110 4.7 66.4 1.0
N A:GLY168 4.7 85.7 1.0
CA A:CYS107 4.7 65.9 1.0
CB A:HIS109 4.8 76.6 1.0
CA A:CYS148 4.8 89.2 1.0
N A:CYS167 5.0 89.2 1.0

Zinc binding site 7 out of 8 in 1twf

Go back to Zinc Binding Sites List in 1twf
Zinc binding site 7 out of 8 in the Rna Polymerase II Complexed with Utp at 2.3 A Resolution


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 7 of Rna Polymerase II Complexed with Utp at 2.3 A Resolution within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Zn3007

b:55.4
occ:1.00
 SG B:CYS1166 2.6 72.1 1.0
SG B:CYS1185 2.7 100.0 1.0
SG B:CYS1182 2.8 0.9 1.0
SG B:CYS1163 2.8 68.0 1.0
CB B:CYS1163 3.1 65.8 1.0
CB B:CYS1185 3.3 0.8 1.0
CB B:CYS1166 3.6 67.8 1.0
CB B:CYS1182 3.9 0.8 1.0
N B:CYS1166 4.0 67.0 1.0
OG1 B:THR1170 4.3 69.7 1.0
CA B:CYS1166 4.4 68.0 1.0
O B:LYS1183 4.5 0.2 1.0
O B:CYS1166 4.6 68.4 1.0
CA B:CYS1163 4.7 65.9 1.0
CB B:ILE1165 4.7 68.6 1.0
CA B:CYS1185 4.7 0.6 1.0
O B:CYS1182 4.7 1.0 1.0
N B:CYS1185 4.9 0.9 1.0
C B:CYS1166 5.0 68.5 1.0

Zinc binding site 8 out of 8 in 1twf

Go back to Zinc Binding Sites List in 1twf
Zinc binding site 8 out of 8 in the Rna Polymerase II Complexed with Utp at 2.3 A Resolution


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 8 of Rna Polymerase II Complexed with Utp at 2.3 A Resolution within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Zn3008

b:97.1
occ:1.00
 SG A:CYS77 2.6 0.5 1.0
NE2 A:HIS80 2.6 96.5 1.0
SG A:CYS70 2.7 0.4 1.0
SG A:CYS67 2.8 0.8 1.0
CD2 A:HIS80 3.3 95.7 1.0
CE1 A:HIS80 3.5 96.0 1.0
CB A:CYS67 3.6 0.3 1.0
O A:CYS67 3.8 0.2 1.0
CB A:CYS77 4.0 0.7 1.0
CB A:CYS70 4.2 0.7 1.0
CG A:HIS80 4.3 95.5 1.0
ND1 A:HIS80 4.4 95.1 1.0
OE1 A:GLU72 4.6 0.1 1.0
C A:CYS67 4.6 0.2 1.0
O A:PRO78 4.7 0.9 1.0
CA A:CYS67 4.8 0.4 1.0
CA A:CYS77 4.8 0.7 1.0
CD A:PRO78 4.9 1.0 1.0
N A:PRO78 4.9 0.5 1.0
O A:GLN71 4.9 0.3 1.0

Reference:

K.D.Westover, D.A.Bushnell, R.D.Kornberg. Structural Basis of Transcription: Nucleotide Selection By Rotation in the Rna Polymerase II Active Center. Cell(Cambridge,Mass.) V. 119 481 2004.
ISSN: ISSN 0092-8674
PubMed: 15537538
DOI: 10.1016/J.CELL.2004.10.016
Page generated: Wed Oct 16 19:19:04 2024

Last articles

Zn in 9J0N
Zn in 9J0O
Zn in 9J0P
Zn in 9FJX
Zn in 9EKB
Zn in 9C0F
Zn in 9CAH
Zn in 9CH0
Zn in 9CH3
Zn in 9CH1
© Copyright 2008-2020 by atomistry.com
Home   |    Site Map   |    Copyright   |    Contact us   |    Privacy