Zinc in PDB 1tt5: Structure of APPBP1-UBA3-UBC12N26: A Unique E1-E2 Interaction Required For Optimal Conjugation of the Ubiquitin-Like Protein NEDD8

The structure of Structure of APPBP1-UBA3-UBC12N26: A Unique E1-E2 Interaction Required For Optimal Conjugation of the Ubiquitin-Like Protein NEDD8, PDB code: 1tt5 was solved by D.T.Huang, D.W.Miller, R.Mathew, R.Cassell, J.M.Holton, M.F.Roussel, B.A.Schulman, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	18.00 / 2.60
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	92.410, 122.770, 195.910, 90.00, 90.00, 90.00
R / Rfree (%)	23.7 / 27.9

The binding sites of Zinc atom in the Structure of APPBP1-UBA3-UBC12N26: A Unique E1-E2 Interaction Required For Optimal Conjugation of the Ubiquitin-Like Protein NEDD8 (pdb code 1tt5). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Structure of APPBP1-UBA3-UBC12N26: A Unique E1-E2 Interaction Required For Optimal Conjugation of the Ubiquitin-Like Protein NEDD8, PDB code: 1tt5:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Structure of APPBP1-UBA3-UBC12N26: A Unique E1-E2 Interaction Required For Optimal Conjugation of the Ubiquitin-Like Protein NEDD8


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Structure of APPBP1-UBA3-UBC12N26: A Unique E1-E2 Interaction Required For Optimal Conjugation of the Ubiquitin-Like Protein NEDD8 within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn1014 b:49.6 occ:1.00 SG B:CYS346 2.2 47.1 1.0 SG B:CYS343 2.4 53.2 1.0 SG B:CYS202 2.4 40.6 1.0 SG B:CYS199 2.6 37.0 1.0 CB B:CYS343 3.3 56.9 1.0 CB B:CYS346 3.3 50.1 1.0 CB B:CYS199 3.5 35.0 1.0 CB B:CYS202 3.6 43.5 1.0 N B:CYS202 3.6 49.8 1.0 N B:CYS346 3.7 50.4 1.0 N B:CYS199 3.9 36.5 1.0 CA B:CYS202 3.9 46.9 1.0 CA B:CYS346 4.1 50.9 1.0 CA B:CYS199 4.2 36.0 1.0 C B:GLU201 4.4 52.7 1.0 CB B:GLU201 4.4 55.0 1.0 C B:CYS199 4.6 36.1 1.0 O B:CYS199 4.6 35.4 1.0 CB B:ALA345 4.7 49.8 1.0 CA B:CYS343 4.7 58.2 1.0 CA B:GLU201 4.8 52.6 1.0 C B:ALA345 4.8 50.2 1.0 N B:GLU201 4.8 48.6 1.0 C B:CYS346 4.8 52.1 1.0 O B:LYS340 5.0 62.3 1.0

Zinc binding site 2 out of 2 in the Structure of APPBP1-UBA3-UBC12N26: A Unique E1-E2 Interaction Required For Optimal Conjugation of the Ubiquitin-Like Protein NEDD8


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Structure of APPBP1-UBA3-UBC12N26: A Unique E1-E2 Interaction Required For Optimal Conjugation of the Ubiquitin-Like Protein NEDD8 within 5.0Å range: probe atom residue distance (Å) B Occ D:Zn1014 b:62.5 occ:1.00 SG D:CYS346 2.2 63.4 1.0 SG D:CYS343 2.4 89.2 1.0 SG D:CYS202 2.4 64.9 1.0 SG D:CYS199 2.6 56.1 1.0 CB D:CYS343 3.2 88.4 1.0 CB D:CYS346 3.2 73.4 1.0 N D:CYS346 3.4 77.0 1.0 N D:CYS202 3.5 71.9 1.0 CB D:CYS202 3.5 68.9 1.0 CB D:CYS199 3.6 53.5 1.0 CA D:CYS202 3.8 71.4 1.0 CA D:CYS346 3.9 76.3 1.0 N D:CYS199 4.0 53.1 1.0 CA D:CYS199 4.3 54.6 1.0 CB D:ALA345 4.3 78.5 1.0 CB D:GLU201 4.4 72.3 1.0 C D:ALA345 4.5 78.0 1.0 C D:GLU201 4.5 71.3 1.0 CA D:CYS343 4.6 88.0 1.0 C D:CYS346 4.7 79.0 1.0 C D:CYS199 4.8 55.9 1.0 CA D:ALA345 4.8 79.2 1.0 O D:CYS199 4.8 56.7 1.0 O D:LYS340 4.8 78.8 1.0 N D:ALA345 4.9 81.2 1.0 O D:CYS343 4.9 86.8 1.0 CA D:GLU201 4.9 69.2 1.0 C D:CYS343 4.9 86.5 1.0 N D:SER347 5.0 83.5 1.0

D.T.Huang, D.W.Miller, R.Mathew, R.Cassell, J.M.Holton, M.F.Roussel, B.A.Schulman. A Unique E1-E2 Interaction Required For Optimal Conjugation of the Ubiquitin-Like Protein NEDD8. Nat.Struct.Mol.Biol. V. 11 927 2004.
ISSN: ISSN 1545-9993
PubMed: 15361859
DOI: 10.1038/NSMB826