Zinc in PDB 1tf9: Streptomyces Griseus Aminopeptidase Complexed with P-Iodo-L- Phenylalanine

The structure of Streptomyces Griseus Aminopeptidase Complexed with P-Iodo-L- Phenylalanine, PDB code: 1tf9 was solved by V.Reiland, R.Gilboa, A.Spungin-Bialik, D.Schomburg, Y.Shoham, S.Blumberg, G.Shoham, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	20.00 / 1.30
Space group	P 41 21 2
Cell size a, b, c (Å), α, β, γ (°)	61.053, 61.053, 144.502, 90.00, 90.00, 90.00
R / Rfree (%)	12.1 / 17.3

The structure of Streptomyces Griseus Aminopeptidase Complexed with P-Iodo-L- Phenylalanine also contains other interesting chemical elements:

Iodine	(I)	1 atom
Calcium	(Ca)	1 atom

The binding sites of Zinc atom in the Streptomyces Griseus Aminopeptidase Complexed with P-Iodo-L- Phenylalanine (pdb code 1tf9). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Streptomyces Griseus Aminopeptidase Complexed with P-Iodo-L- Phenylalanine, PDB code: 1tf9:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Streptomyces Griseus Aminopeptidase Complexed with P-Iodo-L- Phenylalanine


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Streptomyces Griseus Aminopeptidase Complexed with P-Iodo-L- Phenylalanine within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn901 b:16.6 occ:1.00 NE2 A:HIS85 2.0 4.7 1.0 OD1 A:ASP160 2.0 6.5 1.0 OD1 A:ASP97 2.1 9.1 1.0 O A:PHI913 2.2 6.0 0.6 CG A:ASP160 2.7 6.6 1.0 OD2 A:ASP160 2.7 7.6 1.0 C A:PHI913 2.8 5.8 0.6 CE1 A:HIS85 3.0 4.9 1.0 CD2 A:HIS85 3.1 5.5 1.0 CG A:ASP97 3.1 5.8 1.0 CA A:PHI913 3.3 5.7 0.6 CB A:PHI913 3.3 10.8 0.6 N A:PHI913 3.4 6.7 0.6 OD2 A:ASP97 3.6 8.0 1.0 OXT A:PHI913 3.6 8.5 0.6 ZN A:ZN902 3.7 9.2 1.0 OE1 A:GLU131 3.9 9.0 1.0 ND1 A:HIS85 4.1 5.3 1.0 CB A:ASN98 4.1 5.0 1.0 CB A:ASP160 4.2 6.3 1.0 CG A:MET161 4.2 7.0 1.0 CG A:HIS85 4.2 5.0 1.0 OE2 A:GLU132 4.2 8.0 1.0 CG A:PHI913 4.3 10.4 0.6 CB A:ASP97 4.4 5.8 1.0 CD A:GLU131 4.4 7.5 1.0 SD A:MET161 4.4 7.3 1.0 OE2 A:GLU131 4.5 10.8 1.0 CD2 A:PHI913 4.5 7.1 0.6 CA A:ASP97 4.6 5.7 1.0 CG A:ASN98 4.7 3.7 1.0 CA A:ASP160 4.8 5.9 1.0 C A:ASP160 4.8 5.8 1.0 C A:ASP97 4.9 5.6 1.0 N A:MET161 5.0 5.4 1.0

Zinc binding site 2 out of 2 in the Streptomyces Griseus Aminopeptidase Complexed with P-Iodo-L- Phenylalanine


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Streptomyces Griseus Aminopeptidase Complexed with P-Iodo-L- Phenylalanine within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn902 b:9.2 occ:1.00 OD2 A:ASP97 1.9 8.0 1.0 OXT A:PHI913 1.9 8.5 0.6 OE2 A:GLU132 2.0 8.0 1.0 NE2 A:HIS247 2.0 8.0 1.0 C A:PHI913 2.5 5.8 0.6 O A:PHI913 2.5 6.0 0.6 OE1 A:GLU132 2.6 9.2 1.0 CD A:GLU132 2.6 7.1 1.0 CG A:ASP97 2.9 5.8 1.0 CD2 A:HIS247 3.0 6.5 1.0 CE1 A:HIS247 3.1 11.1 1.0 OD1 A:ASP97 3.3 9.1 1.0 ZN A:ZN901 3.7 16.6 1.0 O A:HOH1007 3.9 8.0 1.0 CE1 A:TYR246 3.9 11.0 1.0 CA A:PHI913 4.0 5.7 0.6 OH A:TYR246 4.1 14.5 1.0 CG A:GLU132 4.1 7.3 1.0 CG A:HIS247 4.1 7.2 1.0 ND1 A:HIS247 4.2 10.6 1.0 CB A:ASP97 4.2 5.8 1.0 OE1 A:GLU131 4.4 9.0 1.0 CZ A:TYR246 4.4 11.7 1.0 CE1 A:HIS85 4.4 4.9 1.0 NE2 A:HIS85 4.5 4.7 1.0 CB A:PHI913 4.6 10.8 0.6 CG1 A:VAL89 4.6 8.3 1.0 CD1 A:TYR246 4.9 8.7 1.0 N A:PHI913 4.9 6.7 0.6

V.Reiland, R.Gilboa, A.Spungin-Bialik, D.Schomburg, Y.Shoham, S.Blumberg, G.Shoham. Binding of Inhibitory Aromatic Amino Acids to Streptomyces Griseus Aminopeptidase. Acta Crystallogr.,Sect.D V. 60 1738 2004.
ISSN: ISSN 0907-4449
PubMed: 15388919
DOI: 10.1107/S0907444904018281