Zinc in PDB 1taf: Drosophila Tbp Associated Factors DTAFII42/DTAFII62 Heterotetramer

The structure of Drosophila Tbp Associated Factors DTAFII42/DTAFII62 Heterotetramer, PDB code: 1taf was solved by X.Xie, T.Kokubo, S.L.Cohen, U.A.Mirza, A.Hoffmann, B.T.Chait, R.G.Roeder, Y.Nakatani, S.K.Burley, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	6.00 / 2.00
Space group	P 41 21 2
Cell size a, b, c (Å), α, β, γ (°)	59.800, 59.800, 111.000, 90.00, 90.00, 90.00
R / Rfree (%)	19.8 / 24.4

The binding sites of Zinc atom in the Drosophila Tbp Associated Factors DTAFII42/DTAFII62 Heterotetramer (pdb code 1taf). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 7 binding sites of Zinc where determined in the Drosophila Tbp Associated Factors DTAFII42/DTAFII62 Heterotetramer, PDB code: 1taf:
Jump to Zinc binding site number: 1; 2; 3; 4; 5; 6; 7;

Zinc binding site 1 out of 7 in the Drosophila Tbp Associated Factors DTAFII42/DTAFII62 Heterotetramer


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Drosophila Tbp Associated Factors DTAFII42/DTAFII62 Heterotetramer within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn2002 b:22.8 occ:1.00 OE2 A:GLU38 2.2 14.4 1.0 O B:HOH1039 2.3 9.8 1.0 CD A:GLU38 3.0 13.5 1.0 OE1 A:GLU38 3.0 14.4 1.0 CB B:SER60 3.8 10.8 1.0 O A:HOH1011 4.2 19.1 1.0 O A:HOH1090 4.3 11.6 1.0 CG2 B:VAL61 4.4 19.4 1.0 CG A:GLU38 4.4 12.1 1.0 NH2 B:ARG62 4.5 41.9 1.0 O B:HOH1043 4.5 37.6 1.0 OG B:SER60 4.6 12.9 1.0 O B:HOH1020 4.7 16.8 1.0 N B:VAL61 4.7 11.7 1.0 CA B:SER60 4.8 10.0 1.0 NE B:ARG62 5.0 44.8 1.0

Zinc binding site 2 out of 7 in the Drosophila Tbp Associated Factors DTAFII42/DTAFII62 Heterotetramer


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Drosophila Tbp Associated Factors DTAFII42/DTAFII62 Heterotetramer within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn2003 b:20.1 occ:1.00 OD1 A:ASP73 2.0 15.9 1.0 OD2 B:ASP27 2.0 13.2 1.0 O B:HOH1042 2.1 17.5 1.0 O A:HOH1002 2.3 2.0 1.0 CG B:ASP27 2.7 15.2 1.0 OD1 B:ASP27 2.8 16.6 1.0 CG A:ASP73 2.8 15.4 1.0 OD2 A:ASP73 3.0 17.0 1.0 O A:HOH1035 3.8 20.2 1.0 O B:HOH1014 4.1 8.7 1.0 O B:HOH1057 4.1 32.9 1.0 N A:LEU74 4.1 7.8 1.0 CB B:ASP27 4.2 8.9 1.0 CB A:ASP73 4.3 11.8 1.0 CB A:LEU74 4.3 8.9 1.0 CB B:SER25 4.3 11.4 1.0 O A:HOH1080 4.4 35.2 1.0 OG B:SER25 4.6 15.4 1.0 CA A:ASP73 4.7 9.0 1.0 O A:HOH1036 4.8 18.8 1.0 CA A:LEU74 4.9 8.1 1.0 C A:ASP73 5.0 8.5 1.0

Zinc binding site 3 out of 7 in the Drosophila Tbp Associated Factors DTAFII42/DTAFII62 Heterotetramer


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 3 of Drosophila Tbp Associated Factors DTAFII42/DTAFII62 Heterotetramer within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn2004 b:35.8 occ:1.00 O A:HOH1047 2.0 33.6 1.0 O A:HOH1048 2.1 36.0 1.0 OD2 A:ASP75 2.2 20.5 1.0 O A:HOH1027 2.3 46.1 1.0 O A:HOH1041 2.5 21.6 1.0 OD1 A:ASP75 2.7 13.1 1.0 CG A:ASP75 2.8 15.1 1.0 OD1 A:ASP76 4.0 11.1 1.0 OD2 A:ASP76 4.3 9.8 1.0 CB A:ASP75 4.3 10.8 1.0 CG A:ASP76 4.5 10.0 1.0 CB A:ASP73 4.5 11.8 1.0 O A:HOH1035 4.7 20.2 1.0 O A:HOH1029 4.7 22.0 1.0 OD2 A:ASP73 4.8 17.0 1.0 CB A:ALA67 4.9 30.1 1.0 CG A:LYS69 5.0 30.1 1.0

Zinc binding site 4 out of 7 in the Drosophila Tbp Associated Factors DTAFII42/DTAFII62 Heterotetramer


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 4 of Drosophila Tbp Associated Factors DTAFII42/DTAFII62 Heterotetramer within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn2005 b:14.9 occ:1.00 OE2 A:GLU82 1.9 11.1 1.0 O A:HOH1101 2.4 7.2 1.0 CD A:GLU82 2.7 11.8 1.0 OE1 A:GLU82 2.7 11.9 1.0 CG A:GLU82 4.1 10.0 1.0 O A:HOH1049 4.3 34.4 1.0

Zinc binding site 5 out of 7 in the Drosophila Tbp Associated Factors DTAFII42/DTAFII62 Heterotetramer


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 5 of Drosophila Tbp Associated Factors DTAFII42/DTAFII62 Heterotetramer within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn2001 b:18.2 occ:1.00 OE2 B:GLU31 2.0 15.3 1.0 OD2 B:ASP35 2.0 10.1 1.0 O B:HOH1001 2.1 3.9 1.0 CG B:ASP35 2.7 9.4 1.0 OD1 B:ASP35 2.8 12.4 1.0 CD B:GLU31 3.0 12.7 1.0 CG B:GLU31 3.3 10.9 1.0 OE1 B:GLU31 4.1 16.3 1.0 CB B:ASP35 4.1 6.1 1.0 O A:HOH1006 4.3 14.4 1.0 O B:HOH1023 4.4 37.2 1.0 O B:GLU31 4.5 8.7 1.0 CG2 A:THR81 4.6 6.5 1.0 OG1 A:THR81 4.8 7.5 1.0 CB B:GLU31 4.8 6.5 1.0 O B:HOH1072 4.8 25.5 1.0 O A:HOH1063 5.0 13.8 1.0 CB A:THR81 5.0 8.4 1.0

Zinc binding site 6 out of 7 in the Drosophila Tbp Associated Factors DTAFII42/DTAFII62 Heterotetramer


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 6 of Drosophila Tbp Associated Factors DTAFII42/DTAFII62 Heterotetramer within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn2006 b:37.9 occ:1.00 O B:HOH1097 3.0 32.5 1.0 O A:HOH1093 4.2 40.7 1.0 O B:HOH1083 4.8 14.2 1.0

Zinc binding site 7 out of 7 in the Drosophila Tbp Associated Factors DTAFII42/DTAFII62 Heterotetramer


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 7 of Drosophila Tbp Associated Factors DTAFII42/DTAFII62 Heterotetramer within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn2007 b:49.1 occ:1.00 O B:HOH1095 2.3 25.1 1.0 NE2 B:GLN45 2.4 24.0 1.0 O B:HOH1107 2.4 24.0 1.0 OE1 B:GLN45 2.8 22.9 1.0 CD B:GLN45 3.0 19.7 1.0 O A:HOH1091 3.1 34.7 1.0 O B:HOH1083 3.4 14.2 1.0 NZ B:LYS49 3.6 22.9 1.0 O B:HOH1098 4.1 41.6 1.0 O A:LEU32 4.4 10.3 1.0 CE B:LYS49 4.4 23.7 1.0 CG B:GLN45 4.5 17.5 1.0 O A:HOH1125 4.8 35.5 1.0

X.Xie, T.Kokubo, S.L.Cohen, U.A.Mirza, A.Hoffmann, B.T.Chait, R.G.Roeder, Y.Nakatani, S.K.Burley. Structural Similarity Between Tafs and the Heterotetrameric Core of the Histone Octamer. Nature V. 380 316 1996.
ISSN: ISSN 0028-0836
PubMed: 8598927
DOI: 10.1038/380316A0