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Zinc in PDB 1sgf: Crystal Structure of 7S Ngf: A Complex of Nerve Growth Factor with Four Binding Proteins (Serine Proteinases)

Enzymatic activity of Crystal Structure of 7S Ngf: A Complex of Nerve Growth Factor with Four Binding Proteins (Serine Proteinases)

All present enzymatic activity of Crystal Structure of 7S Ngf: A Complex of Nerve Growth Factor with Four Binding Proteins (Serine Proteinases):
3.4.21.35;

Protein crystallography data

The structure of Crystal Structure of 7S Ngf: A Complex of Nerve Growth Factor with Four Binding Proteins (Serine Proteinases), PDB code: 1sgf was solved by B.D.V.Bax, T.L.Blundell, J.Murray-Rust, N.Q.Mcdonald, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 8.00 / 3.15
Space group P 21 21 21
Cell size a, b, c (Å), α, β, γ (°) 95.680, 96.590, 147.000, 90.00, 90.00, 90.00
R / Rfree (%) 24.6 / 28.2

Zinc Binding Sites:

The binding sites of Zinc atom in the Crystal Structure of 7S Ngf: A Complex of Nerve Growth Factor with Four Binding Proteins (Serine Proteinases) (pdb code 1sgf). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Crystal Structure of 7S Ngf: A Complex of Nerve Growth Factor with Four Binding Proteins (Serine Proteinases), PDB code: 1sgf:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in 1sgf

Go back to Zinc Binding Sites List in 1sgf
Zinc binding site 1 out of 2 in the Crystal Structure of 7S Ngf: A Complex of Nerve Growth Factor with Four Binding Proteins (Serine Proteinases)


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Crystal Structure of 7S Ngf: A Complex of Nerve Growth Factor with Four Binding Proteins (Serine Proteinases) within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Zn1

b:2.0
occ:1.00
NE2 A:HIS82 1.8 19.7 1.0
OE1 A:GLU75 1.9 27.9 1.0
OE1 G:GLU222 2.1 27.3 1.0
NE2 G:HIS217 2.2 46.8 1.0
OE2 G:GLU222 2.6 27.3 1.0
CD G:GLU222 2.7 27.3 1.0
CD A:GLU75 2.7 27.9 1.0
CD2 A:HIS82 2.9 19.7 1.0
CE1 A:HIS82 2.9 19.7 1.0
OE2 A:GLU75 3.0 27.9 1.0
CD2 G:HIS217 3.2 46.8 1.0
CE1 G:HIS217 3.2 46.8 1.0
CD G:PRO219 3.9 6.0 1.0
ND1 A:HIS82 4.0 19.7 1.0
CG A:HIS82 4.0 19.7 1.0
CG A:GLU75 4.1 27.9 1.0
CG G:GLU222 4.2 27.3 1.0
ND1 G:HIS217 4.3 46.8 1.0
CG G:HIS217 4.3 46.8 1.0
CB A:GLU75 4.5 27.9 1.0
O G:THR218 4.8 44.2 1.0
N G:PRO219 4.8 34.3 1.0
CB G:GLU222 4.9 27.3 1.0
CG G:PRO219 4.9 6.0 1.0

Zinc binding site 2 out of 2 in 1sgf

Go back to Zinc Binding Sites List in 1sgf
Zinc binding site 2 out of 2 in the Crystal Structure of 7S Ngf: A Complex of Nerve Growth Factor with Four Binding Proteins (Serine Proteinases)


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Crystal Structure of 7S Ngf: A Complex of Nerve Growth Factor with Four Binding Proteins (Serine Proteinases) within 5.0Å range:
probe atom residue distance (Å) B Occ
X:Zn501

b:2.0
occ:1.00
OD2 X:ASP77 1.8 15.4 1.0
NE2 X:HIS82 1.8 31.9 1.0
NE2 Z:HIS217 1.9 20.6 1.0
OE1 Z:GLU222 2.3 2.3 1.0
CG X:ASP77 2.6 15.4 1.0
CE1 X:HIS82 2.7 31.9 1.0
OE2 Z:GLU222 2.7 2.3 1.0
CD Z:GLU222 2.8 2.3 1.0
CD2 Z:HIS217 2.9 20.6 1.0
CD2 X:HIS82 2.9 31.9 1.0
CE1 Z:HIS217 2.9 20.6 1.0
OD1 X:ASP77 3.3 15.4 1.0
CB X:ASP77 3.5 15.4 1.0
ND1 X:HIS82 3.8 31.9 1.0
CG X:HIS82 4.0 31.9 1.0
CG Z:HIS217 4.0 20.6 1.0
ND1 Z:HIS217 4.0 20.6 1.0
CD Z:PRO219 4.1 5.3 1.0
CG Z:GLU222 4.3 2.3 1.0
CE Z:MET224 4.5 23.3 1.0
O Z:THR218 4.7 3.9 1.0
N Z:PRO219 4.8 13.8 1.0
CB Z:GLU222 4.9 2.3 1.0
CA X:ASP77 4.9 0.9 1.0

Reference:

B.Bax, T.L.Blundell, J.Murray-Rust, N.Q.Mcdonald. Structure of Mouse 7S Ngf: A Complex of Nerve Growth Factor with Four Binding Proteins. Structure V. 5 1275 1997.
ISSN: ISSN 0969-2126
PubMed: 9351801
DOI: 10.1016/S0969-2126(97)00280-3
Page generated: Mon Jan 25 16:13:23 2021

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