Zinc in PDB 1qj0: Human Insulin Hexamers with Chain B His Mutated to Tyr

The structure of Human Insulin Hexamers with Chain B His Mutated to Tyr, PDB code: 1qj0 was solved by L.Tang, J.L.Whittingham, C.S.Verma, L.S.D.Caves, G.G.Dodson, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	25.70 / 2.40
Space group	H 3
Cell size a, b, c (Å), α, β, γ (°)	80.940, 80.940, 37.630, 90.00, 90.00, 120.00
R / Rfree (%)	19.9 / n/a

The structure of Human Insulin Hexamers with Chain B His Mutated to Tyr also contains other interesting chemical elements:

Chlorine

(Cl)

1 atom

The binding sites of Zinc atom in the Human Insulin Hexamers with Chain B His Mutated to Tyr (pdb code 1qj0). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Human Insulin Hexamers with Chain B His Mutated to Tyr, PDB code: 1qj0:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Human Insulin Hexamers with Chain B His Mutated to Tyr


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Human Insulin Hexamers with Chain B His Mutated to Tyr within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn1001 b:11.0 occ:0.33 NE2 B:HIS10 2.0 13.2 1.0 CL B:CL1002 2.1 15.3 0.3 CE1 B:HIS10 2.8 13.7 1.0 CD2 B:HIS10 3.1 15.4 1.0 ND1 B:HIS10 4.0 11.5 1.0 CG B:HIS10 4.2 12.8 1.0 O B:LEU6 4.6 16.9 1.0

Zinc binding site 2 out of 2 in the Human Insulin Hexamers with Chain B His Mutated to Tyr


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Human Insulin Hexamers with Chain B His Mutated to Tyr within 5.0Å range: probe atom residue distance (Å) B Occ D:Zn1001 b:15.0 occ:0.33 O D:HOH2009 1.8 27.4 0.3 NE2 D:HIS10 2.0 13.0 1.0 CE1 D:HIS10 2.9 12.7 1.0 CD2 D:HIS10 3.1 13.7 1.0 ND1 D:HIS10 4.0 13.6 1.0 O D:HOH2010 4.1 28.1 1.0 CG D:HIS10 4.1 16.0 1.0 O B:HOH2003 4.6 23.9 0.3

L.Tang, J.L.Whittingham, C.S.Verma, L.S.D.Caves, G.G.Dodson. Structural Consequences of the B5 Histidine --> Tyrosine Mutation in Human Insulin Characterized By X-Ray Crystallography and Conformational Analysis. Biochemistry V. 38 12041 1999.
ISSN: ISSN 0006-2960
PubMed: 10508408
DOI: 10.1021/BI990700K