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Zinc in PDB 1p91: Crystal Structure of Rlma(I) Enzyme: 23S Rrna N1-G745 Methyltransferase (Northeast Structural Genomics Consortium Target ER19)

Enzymatic activity of Crystal Structure of Rlma(I) Enzyme: 23S Rrna N1-G745 Methyltransferase (Northeast Structural Genomics Consortium Target ER19)

All present enzymatic activity of Crystal Structure of Rlma(I) Enzyme: 23S Rrna N1-G745 Methyltransferase (Northeast Structural Genomics Consortium Target ER19):
2.1.1.51;

Protein crystallography data

The structure of Crystal Structure of Rlma(I) Enzyme: 23S Rrna N1-G745 Methyltransferase (Northeast Structural Genomics Consortium Target ER19), PDB code: 1p91 was solved by K.Das, T.Acton, G.Montelione, E.Arnold, Northeast Structural Genomicsconsortium (Nesg), with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 20.00 / 2.80
Space group I 2 2 2
Cell size a, b, c (Å), α, β, γ (°) 107.187, 122.279, 143.138, 90.00, 90.00, 90.00
R / Rfree (%) 24.8 / 29.6

Zinc Binding Sites:

The binding sites of Zinc atom in the Crystal Structure of Rlma(I) Enzyme: 23S Rrna N1-G745 Methyltransferase (Northeast Structural Genomics Consortium Target ER19) (pdb code 1p91). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Crystal Structure of Rlma(I) Enzyme: 23S Rrna N1-G745 Methyltransferase (Northeast Structural Genomics Consortium Target ER19), PDB code: 1p91:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in 1p91

Go back to Zinc Binding Sites List in 1p91
Zinc binding site 1 out of 2 in the Crystal Structure of Rlma(I) Enzyme: 23S Rrna N1-G745 Methyltransferase (Northeast Structural Genomics Consortium Target ER19)


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Crystal Structure of Rlma(I) Enzyme: 23S Rrna N1-G745 Methyltransferase (Northeast Structural Genomics Consortium Target ER19) within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Zn1301

b:62.8
occ:1.00
ND1 A:HIS25 1.8 42.0 1.0
SG A:CYS8 1.8 34.9 1.0
SG A:CYS5 2.3 38.6 1.0
SG A:CYS21 2.4 35.1 1.0
CE1 A:HIS25 2.7 39.2 1.0
CG A:HIS25 2.9 37.0 1.0
CB A:CYS8 3.1 45.5 1.0
CB A:CYS5 3.2 41.7 1.0
N A:CYS8 3.3 48.0 1.0
CB A:HIS25 3.3 36.2 1.0
CA A:CYS8 3.7 48.5 1.0
CB A:CYS21 3.8 31.8 1.0
NE2 A:HIS25 3.9 38.0 1.0
CD2 A:HIS25 3.9 38.7 1.0
CB A:LEU7 4.3 44.7 1.0
C A:LEU7 4.3 49.0 1.0
C A:CYS8 4.6 48.2 1.0
CB A:GLN23 4.6 45.5 1.0
CA A:CYS5 4.6 40.6 1.0
CA A:HIS25 4.6 40.4 1.0
CA A:LEU7 4.7 47.1 1.0
N A:HIS9 4.8 50.5 1.0
N A:HIS25 4.8 40.7 1.0
N A:LEU7 4.9 45.5 1.0
N A:GLN23 5.0 44.1 1.0

Zinc binding site 2 out of 2 in 1p91

Go back to Zinc Binding Sites List in 1p91
Zinc binding site 2 out of 2 in the Crystal Structure of Rlma(I) Enzyme: 23S Rrna N1-G745 Methyltransferase (Northeast Structural Genomics Consortium Target ER19)


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Crystal Structure of Rlma(I) Enzyme: 23S Rrna N1-G745 Methyltransferase (Northeast Structural Genomics Consortium Target ER19) within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Zn2301

b:96.0
occ:1.00
SG B:CYS21 2.2 79.9 1.0
SG B:CYS8 2.2 91.0 1.0
SG B:CYS5 2.3 96.0 1.0
ND1 B:HIS25 2.3 91.7 1.0
CB B:CYS8 3.0 81.2 1.0
N B:CYS8 3.1 78.7 1.0
CE1 B:HIS25 3.2 95.0 1.0
CG B:HIS25 3.3 93.5 1.0
CB B:CYS5 3.4 82.7 1.0
CB B:GLN23 3.6 92.2 1.0
CB B:HIS25 3.7 91.2 1.0
CA B:CYS8 3.7 82.4 1.0
CB B:LEU7 3.8 75.3 1.0
O B:HOH2402 3.9 72.0 1.0
CB B:CYS21 3.9 82.8 1.0
CG B:GLN23 4.0 93.5 1.0
C B:LEU7 4.2 79.4 1.0
NE2 B:HIS25 4.3 96.6 1.0
CA B:LEU7 4.4 79.4 1.0
CD2 B:HIS25 4.4 95.5 1.0
C B:CYS8 4.6 83.2 1.0
N B:LEU7 4.7 78.5 1.0
CA B:CYS5 4.8 78.8 1.0
OE1 B:GLN23 4.8 95.5 1.0
N B:HIS9 4.9 83.7 1.0
CG B:LEU7 4.9 75.8 1.0
CD1 B:LEU7 4.9 74.7 1.0
CD B:GLN23 5.0 95.2 1.0
CA B:GLN23 5.0 93.6 1.0

Reference:

K.Das, T.Acton, Y.Chiang, L.Shih, E.Arnold, G.T.Montelione. Crystal Structure of Rlmai: Implications For Understanding the 23S Rrna G745/G748-Methylation at the Macrolide Antibiotic-Binding Site. Proc.Natl.Acad.Sci.Usa V. 101 4041 2004.
ISSN: ISSN 0027-8424
PubMed: 14999102
DOI: 10.1073/PNAS.0400189101
Page generated: Thu Oct 29 07:30:30 2020
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