Zinc in PDB 1oep: Structure of Trypanosoma Brucei Enolase Reveals the Inhibitory Divalent Metal Site

All present enzymatic activity of Structure of Trypanosoma Brucei Enolase Reveals the Inhibitory Divalent Metal Site:
4.2.1.11;

The structure of Structure of Trypanosoma Brucei Enolase Reveals the Inhibitory Divalent Metal Site, PDB code: 1oep was solved by M.T.Da Silva Giotto, M.V.A.S.Navarro, R.C.Garratt, D.J.Rigden, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	38.82 / 2.3
Space group	C 2 2 21
Cell size a, b, c (Å), α, β, γ (°)	74.018, 110.536, 109.098, 90.00, 90.00, 90.00
R / Rfree (%)	21 / 25.1

The binding sites of Zinc atom in the Structure of Trypanosoma Brucei Enolase Reveals the Inhibitory Divalent Metal Site (pdb code 1oep). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Structure of Trypanosoma Brucei Enolase Reveals the Inhibitory Divalent Metal Site, PDB code: 1oep:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Structure of Trypanosoma Brucei Enolase Reveals the Inhibitory Divalent Metal Site


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Structure of Trypanosoma Brucei Enolase Reveals the Inhibitory Divalent Metal Site within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn500 b:46.9 occ:1.00 OE2 A:GLU291 2.2 39.6 1.0 OD2 A:ASP318 2.4 43.0 1.0 O A:HOH2234 2.5 40.3 1.0 OD2 A:ASP243 2.6 54.9 1.0 OD1 A:ASP243 2.9 56.7 1.0 CG A:ASP243 3.1 53.7 1.0 CG A:ASP318 3.2 40.2 1.0 CD A:GLU291 3.2 42.8 1.0 CB A:ASP318 3.6 41.2 1.0 OD2 A:ASP292 3.6 47.4 1.0 O A:HOH2235 3.8 34.3 1.0 OE1 A:GLU291 4.0 40.4 1.0 CG A:GLU291 4.1 42.5 1.0 OD1 A:ASP318 4.2 40.5 1.0 NZ A:LYS394 4.3 39.1 1.0 CD2 A:LEU341 4.4 27.0 1.0 OE1 A:GLN164 4.5 50.9 1.0 CB A:ASP243 4.5 47.8 1.0 OE2 A:GLU165 4.7 70.4 1.0 CG A:ASP292 4.8 35.7 1.0 NZ A:LYS343 4.8 42.7 1.0

Zinc binding site 2 out of 2 in the Structure of Trypanosoma Brucei Enolase Reveals the Inhibitory Divalent Metal Site


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Structure of Trypanosoma Brucei Enolase Reveals the Inhibitory Divalent Metal Site within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn501 b:65.5 occ:0.70 OE2 A:GLU208 2.3 64.5 1.0 OE2 A:GLU165 2.3 70.4 1.0 CD A:GLU165 3.0 69.1 1.0 NE2 A:HIS156 3.0 71.9 1.0 CG A:GLU165 3.0 66.1 1.0 OE1 A:GLN164 3.1 50.9 1.0 CD A:GLU208 3.4 58.5 1.0 CD2 A:HIS156 3.4 69.9 1.0 OE1 A:GLU208 3.8 55.9 1.0 CD A:GLN164 3.8 50.7 1.0 CG A:GLN164 3.9 40.9 1.0 OE1 A:GLU165 4.1 70.2 1.0 CE1 A:HIS156 4.2 74.0 1.0 O4 A:SO4600 4.4 68.0 1.0 CB A:GLU165 4.5 58.1 1.0 O A:HOH2234 4.6 40.3 1.0 CG A:GLU208 4.6 52.9 1.0 CG A:HIS156 4.7 68.0 1.0 OD2 A:ASP243 4.7 54.9 1.0 OD1 A:ASN149 4.7 61.1 1.0

M.T.Da Silva Giotto, V.Hannaert, D.Vertommen, M.V.A.S.Navarro, M.H.Rider, P.A.M.Michels, R.C.Garratt, D.J.Rigden. The Crystal Structure of Trypanosoma Brucei Enolase: Visualisation of the Inhibitory Metal Binding Site III and Potential As Target For Selective, Irreversible Inhibition J.Mol.Biol. V. 331 653 2003.
ISSN: ISSN 0022-2836
PubMed: 12899835
DOI: 10.1016/S0022-2836(03)00752-6