Zinc in PDB 1ocr: Bovine Heart Cytochrome C Oxidase in the Fully Reduced State

All present enzymatic activity of Bovine Heart Cytochrome C Oxidase in the Fully Reduced State:
1.9.3.1;

The structure of Bovine Heart Cytochrome C Oxidase in the Fully Reduced State, PDB code: 1ocr was solved by T.Tsukihara, M.Yao, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	15.00 / 2.35
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	189.100, 210.500, 178.600, 90.00, 90.00, 90.00
R / Rfree (%)	20.3 / 24.7

The structure of Bovine Heart Cytochrome C Oxidase in the Fully Reduced State also contains other interesting chemical elements:

Magnesium	(Mg)	2 atoms
Iron	(Fe)	4 atoms
Copper	(Cu)	6 atoms
Sodium	(Na)	2 atoms

The binding sites of Zinc atom in the Bovine Heart Cytochrome C Oxidase in the Fully Reduced State (pdb code 1ocr). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Bovine Heart Cytochrome C Oxidase in the Fully Reduced State, PDB code: 1ocr:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Bovine Heart Cytochrome C Oxidase in the Fully Reduced State


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Bovine Heart Cytochrome C Oxidase in the Fully Reduced State within 5.0Å range: probe atom residue distance (Å) B Occ F:Zn99 b:30.3 occ:1.00 SG F:CYS82 2.1 27.9 1.0 SG F:CYS60 2.1 20.4 1.0 SG F:CYS85 2.2 31.0 1.0 SG F:CYS62 2.2 25.2 1.0 CB F:CYS60 3.0 12.1 1.0 CB F:CYS82 3.0 30.3 1.0 CB F:CYS62 3.5 24.1 1.0 CB F:CYS85 3.6 26.9 1.0 CA F:CYS62 3.7 27.8 1.0 N F:CYS85 3.9 31.4 1.0 N F:CYS62 4.0 25.6 1.0 O F:CYS60 4.0 20.5 1.0 CA F:CYS60 4.2 10.9 1.0 C F:CYS60 4.2 16.8 1.0 CA F:CYS85 4.3 30.1 1.0 CA F:CYS82 4.5 26.1 1.0 CB F:SER84 4.6 37.3 1.0 C F:SER84 4.8 30.4 1.0 C F:ILE61 4.8 25.5 1.0 OG F:SER84 4.9 40.6 1.0 OH F:TYR89 4.9 22.8 1.0 CB F:ILE70 4.9 26.5 1.0 CB F:THR87 4.9 24.8 1.0 N F:ILE61 5.0 23.0 1.0

Zinc binding site 2 out of 2 in the Bovine Heart Cytochrome C Oxidase in the Fully Reduced State


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Bovine Heart Cytochrome C Oxidase in the Fully Reduced State within 5.0Å range: probe atom residue distance (Å) B Occ S:Zn99 b:34.6 occ:1.00 SG S:CYS85 2.1 35.5 1.0 SG S:CYS60 2.2 31.5 1.0 SG S:CYS82 2.2 36.8 1.0 SG S:CYS62 2.2 33.7 1.0 CB S:CYS82 2.9 38.5 1.0 CB S:CYS60 3.1 25.9 1.0 CB S:CYS85 3.5 37.3 1.0 CB S:CYS62 3.5 29.2 1.0 CA S:CYS62 3.8 33.7 1.0 N S:CYS85 3.8 37.8 1.0 N S:CYS62 4.1 30.1 1.0 O S:CYS60 4.2 30.3 1.0 CA S:CYS85 4.2 35.8 1.0 CA S:CYS60 4.3 25.4 1.0 C S:CYS60 4.3 28.4 1.0 CA S:CYS82 4.4 36.2 1.0 CB S:SER84 4.6 38.4 1.0 C S:SER84 4.8 38.3 1.0 CB S:THR87 4.9 33.7 1.0 OG S:SER84 4.9 41.1 1.0 OH S:TYR89 4.9 37.0 1.0 CB S:ILE70 4.9 26.6 1.0 C S:ILE61 4.9 30.0 1.0 C S:CYS85 5.0 37.6 1.0

S.Yoshikawa, K.Shinzawa-Itoh, R.Nakashima, R.Yaono, E.Yamashita, N.Inoue, M.Yao, M.J.Fei, C.P.Libeu, T.Mizushima, H.Yamaguchi, T.Tomizaki, T.Tsukihara. Redox-Coupled Crystal Structural Changes in Bovine Heart Cytochrome C Oxidase. Science V. 280 1723 1998.
ISSN: ISSN 0036-8075
PubMed: 9624044
DOI: 10.1126/SCIENCE.280.5370.1723