Zinc in PDB 1njg: Nucleotide-Free Form of An Isolated E. Coli Clamp Loader Gamma Subunit

All present enzymatic activity of Nucleotide-Free Form of An Isolated E. Coli Clamp Loader Gamma Subunit:
2.7.7.7;

The structure of Nucleotide-Free Form of An Isolated E. Coli Clamp Loader Gamma Subunit, PDB code: 1njg was solved by M.Podobnik, T.F.Weitze, M.O'donnell, J.Kuriyan, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	56.85 / 2.20
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	47.335, 85.471, 56.932, 90.00, 93.15, 90.00
R / Rfree (%)	23.8 / 26.8

The binding sites of Zinc atom in the Nucleotide-Free Form of An Isolated E. Coli Clamp Loader Gamma Subunit (pdb code 1njg). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Nucleotide-Free Form of An Isolated E. Coli Clamp Loader Gamma Subunit, PDB code: 1njg:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Nucleotide-Free Form of An Isolated E. Coli Clamp Loader Gamma Subunit


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Nucleotide-Free Form of An Isolated E. Coli Clamp Loader Gamma Subunit within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn401 b:26.7 occ:1.00 SG A:CYS79 2.3 32.7 1.0 SG A:CYS76 2.3 24.7 1.0 SG A:CYS73 2.3 24.5 1.0 SG A:CYS64 2.4 26.9 1.0 CB A:CYS79 3.3 34.3 1.0 CB A:CYS64 3.4 24.8 1.0 CB A:CYS76 3.5 28.6 1.0 CB A:CYS73 3.6 23.1 1.0 N A:GLY74 3.6 27.3 1.0 N A:CYS73 3.7 23.6 1.0 CA A:CYS64 3.7 26.4 1.0 N A:GLU65 3.9 25.1 1.0 N A:CYS76 4.0 27.8 1.0 CA A:CYS73 4.0 25.7 1.0 C A:CYS73 4.2 25.7 1.0 C A:CYS64 4.3 25.5 1.0 CA A:CYS76 4.3 27.4 1.0 CA A:CYS79 4.4 33.6 1.0 N A:CYS79 4.4 33.8 1.0 O A:HOH625 4.5 35.1 1.0 CA A:GLY74 4.5 28.8 1.0 N A:VAL75 4.5 28.7 1.0 C A:PRO72 4.6 25.0 1.0 CG2 A:THR66 4.9 32.2 1.0 C A:GLY74 4.9 28.5 1.0 CA A:PRO72 4.9 23.9 1.0 N A:CYS64 4.9 27.1 1.0 N A:THR66 5.0 26.8 1.0 C A:CYS76 5.0 27.8 1.0

Zinc binding site 2 out of 2 in the Nucleotide-Free Form of An Isolated E. Coli Clamp Loader Gamma Subunit


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Nucleotide-Free Form of An Isolated E. Coli Clamp Loader Gamma Subunit within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn402 b:25.4 occ:1.00 SG B:CYS64 2.3 20.5 1.0 SG B:CYS73 2.4 24.4 1.0 SG B:CYS79 2.4 22.5 1.0 SG B:CYS76 2.4 24.6 1.0 CB B:CYS73 3.4 20.6 1.0 CB B:CYS64 3.5 21.3 1.0 CB B:CYS76 3.5 25.6 1.0 CB B:CYS79 3.5 27.6 1.0 N B:CYS73 3.5 21.9 1.0 N B:GLY74 3.7 23.1 1.0 CA B:CYS64 3.8 25.3 1.0 CA B:CYS73 3.8 23.7 1.0 N B:CYS76 3.9 23.1 1.0 N B:GLU65 4.0 27.3 1.0 C B:CYS73 4.2 23.2 1.0 CA B:CYS76 4.3 24.5 1.0 C B:CYS64 4.3 24.3 1.0 C B:PRO72 4.5 24.2 1.0 N B:VAL75 4.5 23.6 1.0 N B:CYS79 4.5 33.1 1.0 CA B:CYS79 4.6 32.7 1.0 O B:HOH512 4.6 27.9 1.0 CA B:GLY74 4.7 21.2 1.0 CG2 B:THR66 4.8 33.1 1.0 CA B:PRO72 4.9 22.7 1.0 N B:THR66 4.9 29.1 1.0 C B:CYS76 5.0 26.4 1.0 C B:GLY74 5.0 23.6 1.0

M.Podobnik, T.F.Weitze, M.O'donnell, J.Kuriyan. Nucleotide-Induced Conformational Changes in An Isolated Escherichia Coli Dna Polymerase III Clamp Loader Subunit Structure V. 11 253 2003.
ISSN: ISSN 0969-2126
PubMed: 12623013
DOI: 10.1016/S0969-2126(03)00027-3