Zinc in PDB 1ht0: Human Gamma-2 Alcohol Dehydrogense

All present enzymatic activity of Human Gamma-2 Alcohol Dehydrogense:
1.1.1.1;

The structure of Human Gamma-2 Alcohol Dehydrogense, PDB code: 1ht0 was solved by M.S.Niederhut, B.J.Gibbons, S.Perez-Miller, T.D.Hurley, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	44.00 / 2.00
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	56.400, 71.500, 92.100, 90.00, 102.90, 90.00
R / Rfree (%)	19.3 / 23.1

The binding sites of Zinc atom in the Human Gamma-2 Alcohol Dehydrogense (pdb code 1ht0). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 4 binding sites of Zinc where determined in the Human Gamma-2 Alcohol Dehydrogense, PDB code: 1ht0:
Jump to Zinc binding site number: 1; 2; 3; 4;

Zinc binding site 1 out of 4 in the Human Gamma-2 Alcohol Dehydrogense


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Human Gamma-2 Alcohol Dehydrogense within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn1375 b:23.8 occ:1.00 SG A:CYS103 2.2 18.6 1.0 SG A:CYS111 2.4 18.1 1.0 SG A:CYS97 2.4 19.4 1.0 SG A:CYS100 2.5 18.7 1.0 CB A:CYS111 3.3 19.6 1.0 CB A:CYS103 3.3 20.4 1.0 CB A:CYS97 3.3 22.5 1.0 N A:CYS97 3.5 20.3 1.0 CB A:CYS100 3.5 20.0 1.0 CA A:CYS111 3.7 19.1 1.0 N A:GLY98 3.8 21.9 1.0 CA A:CYS97 3.8 22.4 1.0 N A:CYS100 4.0 22.7 1.0 N A:LEU112 4.0 19.4 1.0 N A:CYS103 4.2 19.5 1.0 C A:CYS97 4.2 22.5 1.0 CA A:CYS103 4.3 21.6 1.0 C A:CYS111 4.3 20.5 1.0 CA A:CYS100 4.3 20.4 1.0 C A:GLN96 4.5 18.7 1.0 N A:LYS99 4.5 25.0 1.0 CD A:LYS113 4.7 37.0 1.0 CA A:GLY98 4.7 23.5 1.0 CA A:GLN96 4.8 18.2 1.0 C A:CYS100 4.9 18.7 1.0 O A:CYS100 4.9 17.9 1.0 N A:LYS113 4.9 24.1 1.0

Zinc binding site 2 out of 4 in the Human Gamma-2 Alcohol Dehydrogense


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Human Gamma-2 Alcohol Dehydrogense within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn1376 b:18.7 occ:1.00 NE2 A:HIS67 2.1 10.8 1.0 SG A:CYS174 2.2 17.7 1.0 O A:HOH1417 2.3 25.6 1.0 SG A:CYS46 2.3 14.6 1.0 CD2 A:HIS67 3.1 14.2 1.0 CE1 A:HIS67 3.1 10.4 1.0 CB A:CYS46 3.2 17.2 1.0 CB A:CYS174 3.4 15.9 1.0 C5N A:NAD1377 3.4 16.8 1.0 C6N A:NAD1377 3.9 15.1 1.0 OG A:SER48 4.0 18.5 1.0 CB A:SER48 4.2 15.1 1.0 C4N A:NAD1377 4.2 18.3 1.0 ND1 A:HIS67 4.3 12.2 1.0 CG A:HIS67 4.3 13.4 1.0 O A:HOH1480 4.4 25.5 1.0 NH2 A:ARG369 4.4 13.5 1.0 OE2 A:GLU68 4.7 21.5 1.0 CA A:CYS46 4.7 12.5 1.0 CA A:CYS174 4.7 15.9 1.0 N A:GLY175 4.8 14.6 1.0 N A:SER48 5.0 11.8 1.0

Zinc binding site 3 out of 4 in the Human Gamma-2 Alcohol Dehydrogense


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 3 of Human Gamma-2 Alcohol Dehydrogense within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn2375 b:22.6 occ:1.00 SG B:CYS103 2.1 21.0 1.0 SG B:CYS97 2.4 19.8 1.0 SG B:CYS111 2.4 15.2 1.0 SG B:CYS100 2.5 19.2 1.0 CB B:CYS111 3.3 16.8 1.0 CB B:CYS103 3.3 21.1 1.0 CB B:CYS97 3.4 22.6 1.0 N B:CYS97 3.5 19.9 1.0 CB B:CYS100 3.5 21.6 1.0 N B:GLY98 3.8 20.8 1.0 CA B:CYS111 3.8 18.7 1.0 CA B:CYS97 3.9 21.3 1.0 N B:CYS100 3.9 22.8 1.0 N B:LEU112 4.1 19.9 1.0 N B:CYS103 4.2 19.1 1.0 C B:CYS97 4.2 21.1 1.0 CA B:CYS100 4.3 20.7 1.0 CA B:CYS103 4.3 20.6 1.0 C B:CYS111 4.4 19.7 1.0 C B:GLN96 4.4 18.5 1.0 N B:LYS99 4.5 23.8 1.0 CA B:GLN96 4.7 16.9 1.0 CA B:GLY98 4.8 23.8 1.0 CG B:LYS113 4.9 23.9 1.0 C B:CYS100 4.9 19.4 1.0 N B:LYS113 4.9 21.9 1.0 O B:CYS100 5.0 19.0 1.0

Zinc binding site 4 out of 4 in the Human Gamma-2 Alcohol Dehydrogense


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 4 of Human Gamma-2 Alcohol Dehydrogense within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn2376 b:18.9 occ:1.00 NE2 B:HIS67 2.1 11.6 1.0 SG B:CYS174 2.2 15.8 1.0 O B:HOH2682 2.3 16.4 1.0 SG B:CYS46 2.3 15.1 1.0 CD2 B:HIS67 3.1 14.3 1.0 CE1 B:HIS67 3.1 10.9 1.0 CB B:CYS46 3.2 14.2 1.0 CB B:CYS174 3.3 15.3 1.0 C5N B:NAD2377 3.4 15.4 1.0 C6N B:NAD2377 4.0 12.9 1.0 OG B:SER48 4.1 17.0 1.0 CB B:SER48 4.2 14.3 1.0 CG B:HIS67 4.2 9.4 1.0 ND1 B:HIS67 4.2 9.2 1.0 C4N B:NAD2377 4.2 17.8 1.0 NH2 B:ARG369 4.4 13.2 1.0 OE2 B:GLU68 4.7 19.4 1.0 CA B:CYS174 4.7 16.0 1.0 CA B:CYS46 4.7 13.3 1.0 O B:HOH2422 4.8 29.1 1.0 N B:GLY175 4.8 14.3 1.0 C B:CYS174 5.0 16.5 1.0 CE1 B:PHE93 5.0 12.8 1.0

M.S.Niederhut, B.J.Gibbons, S.Perez-Miller, T.D.Hurley. Three-Dimensional Structures of the Three Human Class I Alcohol Dehydrogenases. Protein Sci. V. 10 697 2001.
ISSN: ISSN 0961-8368
PubMed: 11274460
DOI: 10.1110/PS.45001