Zinc in PDB 1et5: Crystal Structure of Nitrite Reductase ASP98ASN Mutant From Alcaligenes Faecalis S-6

All present enzymatic activity of Crystal Structure of Nitrite Reductase ASP98ASN Mutant From Alcaligenes Faecalis S-6:
1.7.99.3;

The structure of Crystal Structure of Nitrite Reductase ASP98ASN Mutant From Alcaligenes Faecalis S-6, PDB code: 1et5 was solved by M.J.Boulanger, M.Kukimoto, M.Nishiyama, S.Horinouchi, M.E.P.Murphy, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	500.00 / 1.90
Space group	H 3
Cell size a, b, c (Å), α, β, γ (°)	127.940, 127.940, 66.426, 90.00, 90.00, 120.00
R / Rfree (%)	18.5 / 21.4

The structure of Crystal Structure of Nitrite Reductase ASP98ASN Mutant From Alcaligenes Faecalis S-6 also contains other interesting chemical elements:

Copper

(Cu)

2 atoms

The binding sites of Zinc atom in the Crystal Structure of Nitrite Reductase ASP98ASN Mutant From Alcaligenes Faecalis S-6 (pdb code 1et5). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Crystal Structure of Nitrite Reductase ASP98ASN Mutant From Alcaligenes Faecalis S-6, PDB code: 1et5:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Crystal Structure of Nitrite Reductase ASP98ASN Mutant From Alcaligenes Faecalis S-6


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Crystal Structure of Nitrite Reductase ASP98ASN Mutant From Alcaligenes Faecalis S-6 within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn504 b:46.2 occ:0.33 O A:HOH524 2.3 33.4 1.0 O A:HOH533 2.4 33.8 1.0 OD1 A:ASP275 4.2 24.5 1.0 O A:HOH673 4.3 51.2 1.0 O A:HOH596 4.4 59.5 1.0 OD2 A:ASP275 4.6 24.5 1.0 CG A:ASP275 4.9 23.8 1.0

Zinc binding site 2 out of 2 in the Crystal Structure of Nitrite Reductase ASP98ASN Mutant From Alcaligenes Faecalis S-6


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Crystal Structure of Nitrite Reductase ASP98ASN Mutant From Alcaligenes Faecalis S-6 within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn505 b:68.6 occ:0.33 OD1 A:ASP251 3.1 22.0 1.0 CG A:ASP251 4.1 16.4 1.0 CB A:ASP251 4.4 19.3 1.0 CZ A:PHE282 4.5 23.1 1.0 NH2 A:ARG253 4.6 17.2 1.0 CE1 A:PHE282 4.7 25.2 1.0

M.J.Boulanger, M.Kukimoto, M.Nishiyama, S.Horinouchi, M.E.Murphy. Catalytic Roles For Two Water Bridged Residues (Asp-98 and His-255) in the Active Site of Copper-Containing Nitrite Reductase. J.Biol.Chem. V. 275 23957 2000.
ISSN: ISSN 0021-9258
PubMed: 10811642
DOI: 10.1074/JBC.M001859200