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Zinc in PDB 1e7d: Endonuclease VII (Endovii) Ffrom Phage T4

Enzymatic activity of Endonuclease VII (Endovii) Ffrom Phage T4

All present enzymatic activity of Endonuclease VII (Endovii) Ffrom Phage T4:
3.1.22.4;

Protein crystallography data

The structure of Endonuclease VII (Endovii) Ffrom Phage T4, PDB code: 1e7d was solved by H.C.A.Raaijmakers, O.Vix, I.Toro, D.Suck, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 10.00 / 2.80
Space group P 1 21 1
Cell size a, b, c (Å), α, β, γ (°) 60.900, 37.400, 74.000, 90.00, 108.20, 90.00
R / Rfree (%) 24.5 / 31.5

Other elements in 1e7d:

The structure of Endonuclease VII (Endovii) Ffrom Phage T4 also contains other interesting chemical elements:

Chlorine (Cl) 3 atoms
Calcium (Ca) 2 atoms

Zinc Binding Sites:

The binding sites of Zinc atom in the Endonuclease VII (Endovii) Ffrom Phage T4 (pdb code 1e7d). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Endonuclease VII (Endovii) Ffrom Phage T4, PDB code: 1e7d:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in 1e7d

Go back to Zinc Binding Sites List in 1e7d
Zinc binding site 1 out of 2 in the Endonuclease VII (Endovii) Ffrom Phage T4


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Endonuclease VII (Endovii) Ffrom Phage T4 within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Zn1158

b:23.8
occ:1.00
SG A:CYS58 2.3 13.6 1.0
SG A:CYS23 2.3 13.7 1.0
SG A:CYS61 2.4 12.9 1.0
SG A:CYS26 2.4 13.3 1.0
CB A:CYS61 3.1 18.8 1.0
CB A:CYS23 3.2 20.9 1.0
CB A:CYS26 3.2 25.4 1.0
CB A:CYS58 3.5 17.4 1.0
N A:CYS26 3.6 26.9 1.0
CA A:CYS26 3.9 27.0 1.0
N A:CYS58 3.9 19.2 1.0
N A:CYS61 4.1 23.3 1.0
CA A:CYS61 4.2 22.4 1.0
CA A:CYS58 4.3 18.5 1.0
C A:ILE25 4.6 27.4 1.0
CA A:CYS23 4.6 22.1 1.0
NH2 A:ARG28 4.7 26.8 1.0
CD2 B:LEU77 4.7 24.9 1.0
C A:CYS26 4.8 28.3 1.0
CB A:ILE25 4.9 24.3 1.0
N A:GLN27 5.0 31.1 1.0

Zinc binding site 2 out of 2 in 1e7d

Go back to Zinc Binding Sites List in 1e7d
Zinc binding site 2 out of 2 in the Endonuclease VII (Endovii) Ffrom Phage T4


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Endonuclease VII (Endovii) Ffrom Phage T4 within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Zn1158

b:21.8
occ:1.00
SG B:CYS58 2.3 17.1 1.0
SG B:CYS23 2.3 13.8 1.0
SG B:CYS26 2.4 10.0 1.0
SG B:CYS61 2.4 14.4 1.0
CB B:CYS61 3.2 19.5 1.0
CB B:CYS23 3.2 23.7 1.0
CB B:CYS26 3.3 16.7 1.0
CB B:CYS58 3.5 16.2 1.0
N B:CYS26 3.8 23.5 1.0
N B:CYS58 4.0 17.7 1.0
CA B:CYS26 4.1 22.7 1.0
N B:CYS61 4.2 16.4 1.0
CA B:CYS58 4.3 18.4 1.0
CA B:CYS61 4.3 16.9 1.0
CA B:CYS23 4.7 24.6 1.0
O B:CYS58 4.7 18.8 1.0
C B:ILE25 4.8 21.6 1.0
C B:CYS58 4.8 17.5 1.0
CD2 A:LEU77 4.9 18.9 1.0

Reference:

H.C.A.Raaijmakers, I.Toro, R.Birkenbihl, B.Kemper, D.Suck. Conformational Flexibility in T4 Endonuclease VII Revealed By Crystallography: Implications For Substrate Binding and Cleavage J.Mol.Biol. V. 308 311 2001.
ISSN: ISSN 0022-2836
PubMed: 11327769
DOI: 10.1006/JMBI.2001.4592
Page generated: Wed Dec 16 02:48:03 2020

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