Zinc in PDB 1e71: Myrosinase From Sinapis Alba with Bound Ascorbate

All present enzymatic activity of Myrosinase From Sinapis Alba with Bound Ascorbate:
3.2.1.147;

The structure of Myrosinase From Sinapis Alba with Bound Ascorbate, PDB code: 1e71 was solved by W.P.Burmeister, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	10.00 / 1.50
Space group	C 2 2 21
Cell size a, b, c (Å), α, β, γ (°)	135.300, 137.200, 80.600, 90.00, 90.00, 90.00
R / Rfree (%)	12.7 / 15.2

The binding sites of Zinc atom in the Myrosinase From Sinapis Alba with Bound Ascorbate (pdb code 1e71). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total only one binding site of Zinc was determined in the Myrosinase From Sinapis Alba with Bound Ascorbate, PDB code: 1e71:

Zinc binding site 1 out of 1 in the Myrosinase From Sinapis Alba with Bound Ascorbate


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Myrosinase From Sinapis Alba with Bound Ascorbate within 5.0Å range: probe atom residue distance (Å) B Occ M:Zn1503 b:11.5 occ:0.50 OD2 M:ASP70 2.0 11.6 1.0 NE2 M:HIS56 2.1 11.0 1.0 CG M:ASP70 2.7 12.4 1.0 OD1 M:ASP70 2.9 11.8 1.0 CE1 M:HIS56 3.0 12.5 1.0 CD2 M:HIS56 3.1 12.9 1.0 O M:HOH2132 3.6 24.5 1.0 CB M:ASP70 4.1 11.9 1.0 ND1 M:HIS56 4.1 12.0 1.0 CG M:HIS56 4.2 11.4 1.0 ND2 M:ASN68 4.4 15.6 1.0 OD1 M:ASN68 4.8 13.7 1.0 O M:HOH2086 4.8 38.5 1.0 CG M:ASN68 4.9 14.3 1.0

W.P.Burmeister, S.Cottaz, P.Rollin, A.Vasella, B.Henrissat. High Resolution X-Ray Crystallography Shows That Ascorbate Is A Cofactor For Myrosinase and Substitutes For the Function of the Catalytic Base J.Biol.Chem. V. 275 39385 2000.
ISSN: ISSN 0021-9258
PubMed: 10978344
DOI: 10.1074/JBC.M006796200