Zinc in PDB 1e67: Zn-Azurin From Pseudomonas Aeruginosa

The structure of Zn-Azurin From Pseudomonas Aeruginosa, PDB code: 1e67 was solved by H.Nar, A.Messerschmidt, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	8.00 / 2.14
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	57.490, 80.650, 110.150, 90.00, 90.00, 90.00
R / Rfree (%)	17.2 / n/a

The binding sites of Zinc atom in the Zn-Azurin From Pseudomonas Aeruginosa (pdb code 1e67). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 4 binding sites of Zinc where determined in the Zn-Azurin From Pseudomonas Aeruginosa, PDB code: 1e67:
Jump to Zinc binding site number: 1; 2; 3; 4;

Zinc binding site 1 out of 4 in the Zn-Azurin From Pseudomonas Aeruginosa


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Zn-Azurin From Pseudomonas Aeruginosa within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn129 b:11.3 occ:1.00 ND1 A:HIS46 2.0 11.1 1.0 ND1 A:HIS117 2.0 9.2 1.0 O A:GLY45 2.2 7.5 1.0 SG A:CYS112 2.3 7.8 1.0 CE1 A:HIS117 3.0 7.2 1.0 CE1 A:HIS46 3.0 10.4 1.0 CG A:HIS46 3.0 9.4 1.0 CG A:HIS117 3.1 9.4 1.0 CA A:HIS46 3.2 6.4 1.0 C A:GLY45 3.3 8.4 1.0 SD A:MET121 3.3 8.7 1.0 CB A:CYS112 3.4 7.6 1.0 CB A:HIS46 3.4 7.4 1.0 CB A:HIS117 3.5 7.7 1.0 N A:HIS46 3.7 8.1 1.0 CB A:PHE114 3.8 8.2 1.0 NE2 A:HIS46 4.1 9.1 1.0 NE2 A:HIS117 4.1 8.5 1.0 CD2 A:HIS46 4.2 11.1 1.0 CE A:MET121 4.2 2.2 1.0 CD2 A:HIS117 4.2 9.3 1.0 C A:HIS46 4.5 7.5 1.0 CA A:GLY45 4.6 9.6 1.0 CG A:PHE114 4.6 8.7 1.0 O A:MET44 4.6 7.9 1.0 N A:ASN47 4.7 6.8 1.0 CA A:CYS112 4.8 8.1 1.0 CG A:MET121 4.8 7.5 1.0 N A:GLY45 4.8 8.1 1.0 C A:MET44 4.9 7.7 1.0 N A:PHE114 4.9 9.2 1.0 CD1 A:PHE114 4.9 9.6 1.0 CA A:PHE114 4.9 11.2 1.0 CA A:HIS117 5.0 10.1 1.0

Zinc binding site 2 out of 4 in the Zn-Azurin From Pseudomonas Aeruginosa


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Zn-Azurin From Pseudomonas Aeruginosa within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn129 b:12.4 occ:1.00 ND1 B:HIS117 2.0 8.4 1.0 ND1 B:HIS46 2.1 11.0 1.0 SG B:CYS112 2.3 6.4 1.0 O B:GLY45 2.3 12.0 1.0 CE1 B:HIS117 2.9 8.1 1.0 CE1 B:HIS46 3.0 11.3 1.0 CG B:HIS46 3.1 12.8 1.0 CG B:HIS117 3.1 7.2 1.0 CA B:HIS46 3.3 10.0 1.0 SD B:MET121 3.3 6.8 1.0 C B:GLY45 3.4 10.6 1.0 CB B:CYS112 3.4 4.7 1.0 CB B:HIS46 3.6 10.5 1.0 CB B:HIS117 3.7 6.8 1.0 CB B:PHE114 3.7 8.0 1.0 N B:HIS46 3.8 10.5 1.0 NE2 B:HIS117 4.1 7.0 1.0 NE2 B:HIS46 4.1 6.0 1.0 CE B:MET121 4.2 2.0 1.0 CD2 B:HIS117 4.2 7.9 1.0 CD2 B:HIS46 4.3 9.3 1.0 CG B:PHE114 4.5 7.9 1.0 C B:HIS46 4.6 10.0 1.0 CA B:GLY45 4.6 12.0 1.0 N B:ASN47 4.7 8.8 1.0 O B:MET44 4.7 11.3 1.0 N B:GLY45 4.8 11.9 1.0 CA B:CYS112 4.8 5.2 1.0 CD1 B:PHE114 4.8 8.0 1.0 N B:PHE114 4.9 9.5 1.0 CG B:MET121 4.9 7.2 1.0 C B:MET44 4.9 13.0 1.0 CA B:PHE114 4.9 8.9 1.0 O B:PHE114 5.0 9.1 1.0

Zinc binding site 3 out of 4 in the Zn-Azurin From Pseudomonas Aeruginosa


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 3 of Zn-Azurin From Pseudomonas Aeruginosa within 5.0Å range: probe atom residue distance (Å) B Occ C:Zn129 b:9.3 occ:1.00 ND1 C:HIS117 2.0 9.4 1.0 ND1 C:HIS46 2.1 8.3 1.0 SG C:CYS112 2.3 7.7 1.0 O C:GLY45 2.4 9.0 1.0 CE1 C:HIS117 2.9 8.2 1.0 CG C:HIS117 3.0 9.9 1.0 CE1 C:HIS46 3.1 6.3 1.0 CG C:HIS46 3.1 4.2 1.0 CA C:HIS46 3.3 7.4 1.0 CB C:HIS117 3.4 7.2 1.0 C C:GLY45 3.4 8.6 1.0 CB C:CYS112 3.5 6.8 1.0 SD C:MET121 3.5 8.7 1.0 CB C:HIS46 3.6 7.7 1.0 N C:HIS46 3.8 7.4 1.0 CB C:PHE114 3.8 4.5 1.0 NE2 C:HIS117 4.0 10.9 1.0 CD2 C:HIS117 4.2 9.1 1.0 NE2 C:HIS46 4.2 5.5 1.0 CE C:MET121 4.2 7.4 1.0 CD2 C:HIS46 4.3 3.3 1.0 C C:HIS46 4.5 8.6 1.0 CG C:PHE114 4.6 7.4 1.0 N C:ASN47 4.7 6.8 1.0 CA C:GLY45 4.7 8.1 1.0 N C:PHE114 4.8 6.0 1.0 CA C:CYS112 4.8 6.3 1.0 CA C:PHE114 4.9 6.4 1.0 N C:GLY45 4.9 9.9 1.0 O C:MET44 4.9 12.1 1.0 CA C:HIS117 5.0 7.0 1.0

Zinc binding site 4 out of 4 in the Zn-Azurin From Pseudomonas Aeruginosa


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 4 of Zn-Azurin From Pseudomonas Aeruginosa within 5.0Å range: probe atom residue distance (Å) B Occ D:Zn129 b:11.0 occ:1.00 ND1 D:HIS117 2.0 8.1 1.0 ND1 D:HIS46 2.1 10.0 1.0 SG D:CYS112 2.3 7.5 1.0 O D:GLY45 2.3 8.4 1.0 CE1 D:HIS117 2.9 7.7 1.0 CG D:HIS117 3.0 5.9 1.0 CE1 D:HIS46 3.0 9.8 1.0 CG D:HIS46 3.2 9.3 1.0 C D:GLY45 3.3 8.1 1.0 CA D:HIS46 3.3 6.9 1.0 SD D:MET121 3.4 9.0 1.0 CB D:HIS117 3.5 4.3 1.0 CB D:CYS112 3.5 8.6 1.0 CB D:HIS46 3.7 6.2 1.0 CB D:PHE114 3.7 4.2 1.0 N D:HIS46 3.7 6.6 1.0 NE2 D:HIS117 4.0 8.9 1.0 CD2 D:HIS117 4.1 5.6 1.0 NE2 D:HIS46 4.2 9.3 1.0 CE D:MET121 4.2 4.6 1.0 CD2 D:HIS46 4.3 8.6 1.0 CG D:PHE114 4.5 4.8 1.0 C D:HIS46 4.6 8.9 1.0 CA D:GLY45 4.7 7.9 1.0 N D:ASN47 4.7 8.2 1.0 N D:PHE114 4.8 8.1 1.0 O D:MET44 4.8 11.3 1.0 CA D:PHE114 4.8 6.9 1.0 CA D:CYS112 4.9 7.9 1.0 CG D:MET121 4.9 6.1 1.0 N D:GLY45 5.0 10.3 1.0 CA D:HIS117 5.0 5.6 1.0

H.Nar, R.Huber, A.Messerschmidt, A.C.Filippou, M.Barth, M.Jaquinod, M.Van De Kamp. Characterization and Crystal Structure of Zinc Azurin, A By-Product of Heterologous Expression in Escherichia Coli of Pseudomonas Aeruginosa Copper Azurin Eur.J.Biochem. V. 205 1123 1992.
ISSN: ISSN 0014-2956
PubMed: 1576995
DOI: 10.1111/J.1432-1033.1992.TB16881.X