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Zinc in PDB 1cpx: Beta Form of Carboxypeptidase A (Residues 3-307) From Bovine Pancreas in An Orthorhombic Crystal Form with Two Zinc Ions in the Active Site.

Enzymatic activity of Beta Form of Carboxypeptidase A (Residues 3-307) From Bovine Pancreas in An Orthorhombic Crystal Form with Two Zinc Ions in the Active Site.

All present enzymatic activity of Beta Form of Carboxypeptidase A (Residues 3-307) From Bovine Pancreas in An Orthorhombic Crystal Form with Two Zinc Ions in the Active Site.:
3.4.17.1;

Protein crystallography data

The structure of Beta Form of Carboxypeptidase A (Residues 3-307) From Bovine Pancreas in An Orthorhombic Crystal Form with Two Zinc Ions in the Active Site., PDB code: 1cpx was solved by J.T.Bukrinsky, M.J.Bjerrum, A.Kadziola, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 8.00 / 2.00
Space group P 21 21 21
Cell size a, b, c (Å), α, β, γ (°) 48.790, 66.850, 96.020, 90.00, 90.00, 90.00
R / Rfree (%) 16.7 / 22.6

Zinc Binding Sites:

The binding sites of Zinc atom in the Beta Form of Carboxypeptidase A (Residues 3-307) From Bovine Pancreas in An Orthorhombic Crystal Form with Two Zinc Ions in the Active Site. (pdb code 1cpx). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Beta Form of Carboxypeptidase A (Residues 3-307) From Bovine Pancreas in An Orthorhombic Crystal Form with Two Zinc Ions in the Active Site., PDB code: 1cpx:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in 1cpx

Go back to Zinc Binding Sites List in 1cpx
Zinc binding site 1 out of 2 in the Beta Form of Carboxypeptidase A (Residues 3-307) From Bovine Pancreas in An Orthorhombic Crystal Form with Two Zinc Ions in the Active Site.


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Beta Form of Carboxypeptidase A (Residues 3-307) From Bovine Pancreas in An Orthorhombic Crystal Form with Two Zinc Ions in the Active Site. within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Zn308

b:13.1
occ:1.00
 O A:OH541 1.8 19.2 1.0
ND1 A:HIS69 2.0 5.8 1.0
ND1 A:HIS196 2.1 9.3 1.0
OE1 A:GLU72 2.2 10.5 1.0
OE2 A:GLU72 2.4 11.9 1.0
CD A:GLU72 2.7 9.7 1.0
CE1 A:HIS69 2.9 5.8 1.0
CE1 A:HIS196 3.0 9.2 1.0
CG A:HIS69 3.1 6.0 1.0
CG A:HIS196 3.1 9.6 1.0
CB A:HIS196 3.5 10.1 1.0
CB A:HIS69 3.5 5.7 1.0
ZN A:ZN309 3.5 15.9 0.5
O A:SER197 4.0 8.9 1.0
O A:HOH402 4.1 5.6 1.0
NE2 A:HIS69 4.1 6.6 1.0
CG A:GLU72 4.2 7.3 1.0
CD2 A:HIS69 4.2 6.2 1.0
NE2 A:HIS196 4.2 9.7 1.0
CD2 A:HIS196 4.2 10.1 1.0
O A:HOH626 4.3 27.6 1.0
CA A:HIS196 4.3 9.9 1.0
N A:SER197 4.4 9.8 1.0
OE2 A:GLU270 4.5 14.4 1.0
O A:HOH478 4.6 22.2 1.0
OE1 A:GLU270 4.8 15.5 1.0
CA A:HIS69 4.8 6.6 1.0
NH1 A:ARG127 4.8 23.0 1.0
C A:HIS196 4.9 9.6 1.0
N A:HIS69 5.0 7.0 1.0
C A:SER197 5.0 9.0 1.0

Zinc binding site 2 out of 2 in 1cpx

Go back to Zinc Binding Sites List in 1cpx
Zinc binding site 2 out of 2 in the Beta Form of Carboxypeptidase A (Residues 3-307) From Bovine Pancreas in An Orthorhombic Crystal Form with Two Zinc Ions in the Active Site.


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Beta Form of Carboxypeptidase A (Residues 3-307) From Bovine Pancreas in An Orthorhombic Crystal Form with Two Zinc Ions in the Active Site. within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Zn309

b:15.9
occ:0.50
 OE2 A:GLU270 1.8 14.4 1.0
O A:OH541 1.8 19.2 1.0
CD A:GLU270 2.7 13.9 1.0
OE1 A:GLU270 2.9 15.5 1.0
ZN A:ZN308 3.5 13.1 1.0
O A:HOH516 3.8 26.8 1.0
O A:HOH579 3.8 35.8 1.0
ND1 A:HIS196 3.9 9.3 1.0
CG A:GLU270 4.0 12.8 1.0
CB A:HIS196 4.1 10.1 1.0
O A:HOH478 4.1 22.2 1.0
O A:SER197 4.2 8.9 1.0
CG A:HIS196 4.3 9.6 1.0
O A:HOH626 4.5 27.6 1.0
C A:SER197 4.7 9.0 1.0
ND1 A:HIS69 4.8 5.8 1.0
CE1 A:HIS196 4.8 9.2 1.0
O A:HOH577 4.8 34.6 1.0
CE1 A:HIS69 4.9 5.8 1.0
OE2 A:GLU72 4.9 11.9 1.0
OE1 A:GLU72 5.0 10.5 1.0
N A:SER197 5.0 9.8 1.0

Reference:

J.T.Bukrinsky, M.J.Bjerrum, A.Kadziola. Native Carboxypeptidase A in A New Crystal Environment Reveals A Different Conformation of the Important Tyrosine 248. Biochemistry V. 37 16555 1998.
ISSN: ISSN 0006-2960
PubMed: 9843422
DOI: 10.1021/BI981678I
Page generated: Mon Jan 25 16:08:07 2021

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