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Zinc in PDB 1cgf: Crystal Structures of Recombinant 19-kDa Human Fibroblast Collagenase Complexed to Itself

Enzymatic activity of Crystal Structures of Recombinant 19-kDa Human Fibroblast Collagenase Complexed to Itself

All present enzymatic activity of Crystal Structures of Recombinant 19-kDa Human Fibroblast Collagenase Complexed to Itself:
3.4.24.7;

Protein crystallography data

The structure of Crystal Structures of Recombinant 19-kDa Human Fibroblast Collagenase Complexed to Itself, PDB code: 1cgf was solved by B.Lovejoy, A.M.Hassell, M.A.Luther, D.Weigl, S.R.Jordan, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 7.00 / 2.10
Space group P 1 21 1
Cell size a, b, c (Å), α, β, γ (°) 71.000, 50.500, 48.000, 90.00, 100.00, 90.00
R / Rfree (%) 19.7 / n/a

Other elements in 1cgf:

The structure of Crystal Structures of Recombinant 19-kDa Human Fibroblast Collagenase Complexed to Itself also contains other interesting chemical elements:

Calcium (Ca) 6 atoms

Zinc Binding Sites:

The binding sites of Zinc atom in the Crystal Structures of Recombinant 19-kDa Human Fibroblast Collagenase Complexed to Itself (pdb code 1cgf). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 4 binding sites of Zinc where determined in the Crystal Structures of Recombinant 19-kDa Human Fibroblast Collagenase Complexed to Itself, PDB code: 1cgf:
Jump to Zinc binding site number: 1; 2; 3; 4;

Zinc binding site 1 out of 4 in 1cgf

Go back to Zinc Binding Sites List in 1cgf
Zinc binding site 1 out of 4 in the Crystal Structures of Recombinant 19-kDa Human Fibroblast Collagenase Complexed to Itself


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Crystal Structures of Recombinant 19-kDa Human Fibroblast Collagenase Complexed to Itself within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Zn301

b:23.7
occ:1.00
 NE2 A:HIS228 2.1 33.1 1.0
NE2 A:HIS218 2.2 24.6 1.0
NE2 A:HIS222 2.2 26.2 1.0
CE1 A:HIS228 3.0 33.1 1.0
CD2 A:HIS222 3.0 24.2 1.0
CD2 A:HIS218 3.1 25.4 1.0
CD2 A:HIS228 3.1 30.6 1.0
CE1 A:HIS218 3.3 24.1 1.0
CE1 A:HIS222 3.3 24.6 1.0
OE1 A:GLU219 3.9 23.2 1.0
ND1 A:HIS228 4.1 34.0 1.0
CG A:HIS228 4.2 32.0 1.0
CG A:HIS218 4.3 26.1 1.0
CG A:HIS222 4.3 24.8 1.0
ND1 A:HIS218 4.3 25.2 1.0
ND1 A:HIS222 4.4 23.8 1.0
OE2 A:GLU219 4.4 22.7 1.0
O A:HOH314 4.5 34.9 1.0
CD A:GLU219 4.5 22.2 1.0
H2 A:HOH314 4.8 0.0 1.0
HD1 A:HIS228 5.0 0.0 1.0

Zinc binding site 2 out of 4 in 1cgf

Go back to Zinc Binding Sites List in 1cgf
Zinc binding site 2 out of 4 in the Crystal Structures of Recombinant 19-kDa Human Fibroblast Collagenase Complexed to Itself


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Crystal Structures of Recombinant 19-kDa Human Fibroblast Collagenase Complexed to Itself within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Zn302

b:29.9
occ:1.00
 NE2 A:HIS168 2.1 27.1 1.0
ND1 A:HIS196 2.1 20.6 1.0
NE2 A:HIS183 2.1 27.7 1.0
OD2 A:ASP170 2.1 29.2 1.0
CE1 A:HIS183 2.7 26.8 1.0
CD2 A:HIS168 2.8 28.0 1.0
CE1 A:HIS196 3.0 18.7 1.0
CG A:HIS196 3.1 20.1 1.0
CG A:ASP170 3.1 32.7 1.0
CE1 A:HIS168 3.2 28.5 1.0
CD2 A:HIS183 3.3 26.4 1.0
CB A:HIS196 3.5 20.7 1.0
OD1 A:ASP170 3.5 33.7 1.0
ND1 A:HIS183 3.9 27.6 1.0
CG A:HIS168 4.0 27.8 1.0
O A:SER172 4.1 32.5 1.0
CZ A:PHE185 4.2 24.3 1.0
NE2 A:HIS196 4.2 20.5 1.0
ND1 A:HIS168 4.2 28.6 1.0
CE1 A:PHE185 4.2 24.0 1.0
CG A:HIS183 4.2 26.3 1.0
CD2 A:HIS196 4.2 21.4 1.0
OG A:SER172 4.4 31.1 1.0
CE2 A:PHE174 4.4 23.4 1.0
CB A:ASP170 4.5 36.2 1.0
CZ A:PHE174 4.7 21.7 1.0
HD1 A:HIS183 4.7 0.0 1.0
H2 A:HOH308 4.7 0.0 1.0
H1 A:HOH308 4.7 0.0 1.0
H A:SER172 4.9 0.0 1.0
CB A:SER172 4.9 32.5 1.0
CA A:HIS196 4.9 21.4 1.0
C A:SER172 5.0 34.0 1.0
O A:HOH308 5.0 41.2 1.0

Zinc binding site 3 out of 4 in 1cgf

Go back to Zinc Binding Sites List in 1cgf
Zinc binding site 3 out of 4 in the Crystal Structures of Recombinant 19-kDa Human Fibroblast Collagenase Complexed to Itself


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 3 of Crystal Structures of Recombinant 19-kDa Human Fibroblast Collagenase Complexed to Itself within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Zn301

b:21.8
occ:1.00
 NE2 B:HIS218 2.0 15.1 1.0
NE2 B:HIS228 2.1 20.1 1.0
NE2 B:HIS222 2.1 17.1 1.0
CD2 B:HIS228 3.0 18.4 1.0
CD2 B:HIS218 3.0 12.8 1.0
CD2 B:HIS222 3.0 16.4 1.0
CE1 B:HIS218 3.1 14.0 1.0
CE1 B:HIS228 3.2 18.9 1.0
CE1 B:HIS222 3.2 14.2 1.0
ND1 B:HIS218 4.2 14.8 1.0
CG B:HIS218 4.2 11.2 1.0
CG B:HIS228 4.2 18.9 1.0
H1 B:HOH312 4.2 0.0 1.0
O B:HOH396 4.2 42.9 1.0
CG B:HIS222 4.2 15.7 1.0
ND1 B:HIS228 4.2 19.3 1.0
OE1 B:GLU219 4.3 19.8 1.0
ND1 B:HIS222 4.3 14.9 1.0
H1 B:HOH396 4.3 0.0 1.0
O B:HOH349 4.4 36.6 1.0
O B:HOH357 4.4 38.7 1.0
OE2 B:GLU219 4.4 21.0 1.0
H2 B:HOH396 4.4 0.0 1.0
O B:HOH312 4.4 24.2 1.0
H2 B:HOH357 4.6 0.0 1.0
CD B:GLU219 4.7 17.0 1.0
H1 B:HOH349 4.7 0.0 1.0
H2 B:HOH349 4.9 0.0 1.0
O B:HOH419 5.0 36.6 1.0

Zinc binding site 4 out of 4 in 1cgf

Go back to Zinc Binding Sites List in 1cgf
Zinc binding site 4 out of 4 in the Crystal Structures of Recombinant 19-kDa Human Fibroblast Collagenase Complexed to Itself


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 4 of Crystal Structures of Recombinant 19-kDa Human Fibroblast Collagenase Complexed to Itself within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Zn302

b:17.4
occ:1.00
 ND1 B:HIS196 2.0 12.5 1.0
OD2 B:ASP170 2.1 17.4 1.0
NE2 B:HIS183 2.1 17.5 1.0
NE2 B:HIS168 2.2 20.7 1.0
CE1 B:HIS196 2.8 12.6 1.0
CG B:ASP170 2.9 20.4 1.0
CE1 B:HIS183 3.0 16.9 1.0
OD1 B:ASP170 3.1 19.8 1.0
CD2 B:HIS168 3.1 19.1 1.0
CE1 B:HIS168 3.2 19.2 1.0
CG B:HIS196 3.2 12.2 1.0
CD2 B:HIS183 3.2 15.9 1.0
CB B:HIS196 3.7 10.7 1.0
NE2 B:HIS196 4.0 13.4 1.0
O B:SER172 4.1 20.1 1.0
CB B:ASP170 4.1 21.7 1.0
CZ B:PHE185 4.2 20.2 1.0
CE1 B:PHE185 4.2 19.2 1.0
CD2 B:HIS196 4.2 11.1 1.0
ND1 B:HIS183 4.2 15.7 1.0
ND1 B:HIS168 4.3 18.3 1.0
CG B:HIS168 4.3 19.9 1.0
CG B:HIS183 4.3 16.4 1.0
H B:SER172 4.7 0.0 1.0
CE2 B:PHE174 4.7 15.4 1.0
CB B:SER172 4.8 19.7 1.0
CZ B:PHE174 4.8 16.6 1.0
C B:SER172 4.9 21.6 1.0
HE2 B:HIS196 4.9 0.0 1.0

Reference:

B.Lovejoy, A.M.Hassell, M.A.Luther, D.Weigl, S.R.Jordan. Crystal Structures of Recombinant 19-kDa Human Fibroblast Collagenase Complexed to Itself. Biochemistry V. 33 8207 1994.
ISSN: ISSN 0006-2960
PubMed: 8031754
DOI: 10.1021/BI00193A006
Page generated: Sat Oct 12 23:05:24 2024

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