Atomistry » Zinc » PDB 1caq-1co4 » 1cgf
Atomistry »
  Zinc »
    PDB 1caq-1co4 »
      1cgf »

Zinc in PDB 1cgf: Crystal Structures of Recombinant 19-kDa Human Fibroblast Collagenase Complexed to Itself

Enzymatic activity of Crystal Structures of Recombinant 19-kDa Human Fibroblast Collagenase Complexed to Itself

All present enzymatic activity of Crystal Structures of Recombinant 19-kDa Human Fibroblast Collagenase Complexed to Itself:
3.4.24.7;

Protein crystallography data

The structure of Crystal Structures of Recombinant 19-kDa Human Fibroblast Collagenase Complexed to Itself, PDB code: 1cgf was solved by B.Lovejoy, A.M.Hassell, M.A.Luther, D.Weigl, S.R.Jordan, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 7.00 / 2.10
Space group P 1 21 1
Cell size a, b, c (Å), α, β, γ (°) 71.000, 50.500, 48.000, 90.00, 100.00, 90.00
R / Rfree (%) 19.7 / n/a

Other elements in 1cgf:

The structure of Crystal Structures of Recombinant 19-kDa Human Fibroblast Collagenase Complexed to Itself also contains other interesting chemical elements:

Calcium (Ca) 6 atoms

Zinc Binding Sites:

The binding sites of Zinc atom in the Crystal Structures of Recombinant 19-kDa Human Fibroblast Collagenase Complexed to Itself (pdb code 1cgf). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 4 binding sites of Zinc where determined in the Crystal Structures of Recombinant 19-kDa Human Fibroblast Collagenase Complexed to Itself, PDB code: 1cgf:
Jump to Zinc binding site number: 1; 2; 3; 4;

Zinc binding site 1 out of 4 in 1cgf

Go back to Zinc Binding Sites List in 1cgf
Zinc binding site 1 out of 4 in the Crystal Structures of Recombinant 19-kDa Human Fibroblast Collagenase Complexed to Itself


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Crystal Structures of Recombinant 19-kDa Human Fibroblast Collagenase Complexed to Itself within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Zn301

b:23.7
occ:1.00
 NE2 A:HIS228 2.1 33.1 1.0
NE2 A:HIS218 2.2 24.6 1.0
NE2 A:HIS222 2.2 26.2 1.0
CE1 A:HIS228 3.0 33.1 1.0
CD2 A:HIS222 3.0 24.2 1.0
CD2 A:HIS218 3.1 25.4 1.0
CD2 A:HIS228 3.1 30.6 1.0
CE1 A:HIS218 3.3 24.1 1.0
CE1 A:HIS222 3.3 24.6 1.0
OE1 A:GLU219 3.9 23.2 1.0
ND1 A:HIS228 4.1 34.0 1.0
CG A:HIS228 4.2 32.0 1.0
CG A:HIS218 4.3 26.1 1.0
CG A:HIS222 4.3 24.8 1.0
ND1 A:HIS218 4.3 25.2 1.0
ND1 A:HIS222 4.4 23.8 1.0
OE2 A:GLU219 4.4 22.7 1.0
O A:HOH314 4.5 34.9 1.0
CD A:GLU219 4.5 22.2 1.0
H2 A:HOH314 4.8 0.0 1.0
HD1 A:HIS228 5.0 0.0 1.0

Zinc binding site 2 out of 4 in 1cgf

Go back to Zinc Binding Sites List in 1cgf
Zinc binding site 2 out of 4 in the Crystal Structures of Recombinant 19-kDa Human Fibroblast Collagenase Complexed to Itself


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Crystal Structures of Recombinant 19-kDa Human Fibroblast Collagenase Complexed to Itself within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Zn302

b:29.9
occ:1.00
 NE2 A:HIS168 2.1 27.1 1.0
ND1 A:HIS196 2.1 20.6 1.0
NE2 A:HIS183 2.1 27.7 1.0
OD2 A:ASP170 2.1 29.2 1.0
CE1 A:HIS183 2.7 26.8 1.0
CD2 A:HIS168 2.8 28.0 1.0
CE1 A:HIS196 3.0 18.7 1.0
CG A:HIS196 3.1 20.1 1.0
CG A:ASP170 3.1 32.7 1.0
CE1 A:HIS168 3.2 28.5 1.0
CD2 A:HIS183 3.3 26.4 1.0
CB A:HIS196 3.5 20.7 1.0
OD1 A:ASP170 3.5 33.7 1.0
ND1 A:HIS183 3.9 27.6 1.0
CG A:HIS168 4.0 27.8 1.0
O A:SER172 4.1 32.5 1.0
CZ A:PHE185 4.2 24.3 1.0
NE2 A:HIS196 4.2 20.5 1.0
ND1 A:HIS168 4.2 28.6 1.0
CE1 A:PHE185 4.2 24.0 1.0
CG A:HIS183 4.2 26.3 1.0
CD2 A:HIS196 4.2 21.4 1.0
OG A:SER172 4.4 31.1 1.0
CE2 A:PHE174 4.4 23.4 1.0
CB A:ASP170 4.5 36.2 1.0
CZ A:PHE174 4.7 21.7 1.0
HD1 A:HIS183 4.7 0.0 1.0
H2 A:HOH308 4.7 0.0 1.0
H1 A:HOH308 4.7 0.0 1.0
H A:SER172 4.9 0.0 1.0
CB A:SER172 4.9 32.5 1.0
CA A:HIS196 4.9 21.4 1.0
C A:SER172 5.0 34.0 1.0
O A:HOH308 5.0 41.2 1.0

Zinc binding site 3 out of 4 in 1cgf

Go back to Zinc Binding Sites List in 1cgf
Zinc binding site 3 out of 4 in the Crystal Structures of Recombinant 19-kDa Human Fibroblast Collagenase Complexed to Itself


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 3 of Crystal Structures of Recombinant 19-kDa Human Fibroblast Collagenase Complexed to Itself within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Zn301

b:21.8
occ:1.00
 NE2 B:HIS218 2.0 15.1 1.0
NE2 B:HIS228 2.1 20.1 1.0
NE2 B:HIS222 2.1 17.1 1.0
CD2 B:HIS228 3.0 18.4 1.0
CD2 B:HIS218 3.0 12.8 1.0
CD2 B:HIS222 3.0 16.4 1.0
CE1 B:HIS218 3.1 14.0 1.0
CE1 B:HIS228 3.2 18.9 1.0
CE1 B:HIS222 3.2 14.2 1.0
ND1 B:HIS218 4.2 14.8 1.0
CG B:HIS218 4.2 11.2 1.0
CG B:HIS228 4.2 18.9 1.0
H1 B:HOH312 4.2 0.0 1.0
O B:HOH396 4.2 42.9 1.0
CG B:HIS222 4.2 15.7 1.0
ND1 B:HIS228 4.2 19.3 1.0
OE1 B:GLU219 4.3 19.8 1.0
ND1 B:HIS222 4.3 14.9 1.0
H1 B:HOH396 4.3 0.0 1.0
O B:HOH349 4.4 36.6 1.0
O B:HOH357 4.4 38.7 1.0
OE2 B:GLU219 4.4 21.0 1.0
H2 B:HOH396 4.4 0.0 1.0
O B:HOH312 4.4 24.2 1.0
H2 B:HOH357 4.6 0.0 1.0
CD B:GLU219 4.7 17.0 1.0
H1 B:HOH349 4.7 0.0 1.0
H2 B:HOH349 4.9 0.0 1.0
O B:HOH419 5.0 36.6 1.0

Zinc binding site 4 out of 4 in 1cgf

Go back to Zinc Binding Sites List in 1cgf
Zinc binding site 4 out of 4 in the Crystal Structures of Recombinant 19-kDa Human Fibroblast Collagenase Complexed to Itself


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 4 of Crystal Structures of Recombinant 19-kDa Human Fibroblast Collagenase Complexed to Itself within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Zn302

b:17.4
occ:1.00
 ND1 B:HIS196 2.0 12.5 1.0
OD2 B:ASP170 2.1 17.4 1.0
NE2 B:HIS183 2.1 17.5 1.0
NE2 B:HIS168 2.2 20.7 1.0
CE1 B:HIS196 2.8 12.6 1.0
CG B:ASP170 2.9 20.4 1.0
CE1 B:HIS183 3.0 16.9 1.0
OD1 B:ASP170 3.1 19.8 1.0
CD2 B:HIS168 3.1 19.1 1.0
CE1 B:HIS168 3.2 19.2 1.0
CG B:HIS196 3.2 12.2 1.0
CD2 B:HIS183 3.2 15.9 1.0
CB B:HIS196 3.7 10.7 1.0
NE2 B:HIS196 4.0 13.4 1.0
O B:SER172 4.1 20.1 1.0
CB B:ASP170 4.1 21.7 1.0
CZ B:PHE185 4.2 20.2 1.0
CE1 B:PHE185 4.2 19.2 1.0
CD2 B:HIS196 4.2 11.1 1.0
ND1 B:HIS183 4.2 15.7 1.0
ND1 B:HIS168 4.3 18.3 1.0
CG B:HIS168 4.3 19.9 1.0
CG B:HIS183 4.3 16.4 1.0
H B:SER172 4.7 0.0 1.0
CE2 B:PHE174 4.7 15.4 1.0
CB B:SER172 4.8 19.7 1.0
CZ B:PHE174 4.8 16.6 1.0
C B:SER172 4.9 21.6 1.0
HE2 B:HIS196 4.9 0.0 1.0

Reference:

B.Lovejoy, A.M.Hassell, M.A.Luther, D.Weigl, S.R.Jordan. Crystal Structures of Recombinant 19-kDa Human Fibroblast Collagenase Complexed to Itself. Biochemistry V. 33 8207 1994.
ISSN: ISSN 0006-2960
PubMed: 8031754
DOI: 10.1021/BI00193A006
Page generated: Sat Oct 12 23:05:24 2024

Last articles

Zn in 9JYW
Zn in 9IR4
Zn in 9IR3
Zn in 9GMX
Zn in 9GMW
Zn in 9JEJ
Zn in 9ERF
Zn in 9ERE
Zn in 9EGV
Zn in 9EGW
© Copyright 2008-2020 by atomistry.com
Home   |    Site Map   |    Copyright   |    Contact us   |    Privacy