Zinc in PDB 1cgf: Crystal Structures of Recombinant 19-kDa Human Fibroblast Collagenase Complexed to Itself
Enzymatic activity of Crystal Structures of Recombinant 19-kDa Human Fibroblast Collagenase Complexed to Itself
All present enzymatic activity of Crystal Structures of Recombinant 19-kDa Human Fibroblast Collagenase Complexed to Itself:
3.4.24.7;
Protein crystallography data
The structure of Crystal Structures of Recombinant 19-kDa Human Fibroblast Collagenase Complexed to Itself, PDB code: 1cgf
was solved by
B.Lovejoy,
A.M.Hassell,
M.A.Luther,
D.Weigl,
S.R.Jordan,
with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:
Resolution Low / High (Å)
|
7.00 /
2.10
|
Space group
|
P 1 21 1
|
Cell size a, b, c (Å), α, β, γ (°)
|
71.000,
50.500,
48.000,
90.00,
100.00,
90.00
|
R / Rfree (%)
|
19.7 /
n/a
|
Other elements in 1cgf:
The structure of Crystal Structures of Recombinant 19-kDa Human Fibroblast Collagenase Complexed to Itself also contains other interesting chemical elements:
Zinc Binding Sites:
The binding sites of Zinc atom in the Crystal Structures of Recombinant 19-kDa Human Fibroblast Collagenase Complexed to Itself
(pdb code 1cgf). This binding sites where shown within
5.0 Angstroms radius around Zinc atom.
In total 4 binding sites of Zinc where determined in the
Crystal Structures of Recombinant 19-kDa Human Fibroblast Collagenase Complexed to Itself, PDB code: 1cgf:
Jump to Zinc binding site number:
1;
2;
3;
4;
Zinc binding site 1 out
of 4 in 1cgf
Go back to
Zinc Binding Sites List in 1cgf
Zinc binding site 1 out
of 4 in the Crystal Structures of Recombinant 19-kDa Human Fibroblast Collagenase Complexed to Itself
Mono view
Stereo pair view
|
A full contact list of Zinc with other atoms in the Zn binding
site number 1 of Crystal Structures of Recombinant 19-kDa Human Fibroblast Collagenase Complexed to Itself within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Zn301
b:23.7
occ:1.00
|
NE2
|
A:HIS228
|
2.1
|
33.1
|
1.0
|
NE2
|
A:HIS218
|
2.2
|
24.6
|
1.0
|
NE2
|
A:HIS222
|
2.2
|
26.2
|
1.0
|
CE1
|
A:HIS228
|
3.0
|
33.1
|
1.0
|
CD2
|
A:HIS222
|
3.0
|
24.2
|
1.0
|
CD2
|
A:HIS218
|
3.1
|
25.4
|
1.0
|
CD2
|
A:HIS228
|
3.1
|
30.6
|
1.0
|
CE1
|
A:HIS218
|
3.3
|
24.1
|
1.0
|
CE1
|
A:HIS222
|
3.3
|
24.6
|
1.0
|
OE1
|
A:GLU219
|
3.9
|
23.2
|
1.0
|
ND1
|
A:HIS228
|
4.1
|
34.0
|
1.0
|
CG
|
A:HIS228
|
4.2
|
32.0
|
1.0
|
CG
|
A:HIS218
|
4.3
|
26.1
|
1.0
|
CG
|
A:HIS222
|
4.3
|
24.8
|
1.0
|
ND1
|
A:HIS218
|
4.3
|
25.2
|
1.0
|
ND1
|
A:HIS222
|
4.4
|
23.8
|
1.0
|
OE2
|
A:GLU219
|
4.4
|
22.7
|
1.0
|
O
|
A:HOH314
|
4.5
|
34.9
|
1.0
|
CD
|
A:GLU219
|
4.5
|
22.2
|
1.0
|
H2
|
A:HOH314
|
4.8
|
0.0
|
1.0
|
HD1
|
A:HIS228
|
5.0
|
0.0
|
1.0
|
|
Zinc binding site 2 out
of 4 in 1cgf
Go back to
Zinc Binding Sites List in 1cgf
Zinc binding site 2 out
of 4 in the Crystal Structures of Recombinant 19-kDa Human Fibroblast Collagenase Complexed to Itself
Mono view
Stereo pair view
|
A full contact list of Zinc with other atoms in the Zn binding
site number 2 of Crystal Structures of Recombinant 19-kDa Human Fibroblast Collagenase Complexed to Itself within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Zn302
b:29.9
occ:1.00
|
NE2
|
A:HIS168
|
2.1
|
27.1
|
1.0
|
ND1
|
A:HIS196
|
2.1
|
20.6
|
1.0
|
NE2
|
A:HIS183
|
2.1
|
27.7
|
1.0
|
OD2
|
A:ASP170
|
2.1
|
29.2
|
1.0
|
CE1
|
A:HIS183
|
2.7
|
26.8
|
1.0
|
CD2
|
A:HIS168
|
2.8
|
28.0
|
1.0
|
CE1
|
A:HIS196
|
3.0
|
18.7
|
1.0
|
CG
|
A:HIS196
|
3.1
|
20.1
|
1.0
|
CG
|
A:ASP170
|
3.1
|
32.7
|
1.0
|
CE1
|
A:HIS168
|
3.2
|
28.5
|
1.0
|
CD2
|
A:HIS183
|
3.3
|
26.4
|
1.0
|
CB
|
A:HIS196
|
3.5
|
20.7
|
1.0
|
OD1
|
A:ASP170
|
3.5
|
33.7
|
1.0
|
ND1
|
A:HIS183
|
3.9
|
27.6
|
1.0
|
CG
|
A:HIS168
|
4.0
|
27.8
|
1.0
|
O
|
A:SER172
|
4.1
|
32.5
|
1.0
|
CZ
|
A:PHE185
|
4.2
|
24.3
|
1.0
|
NE2
|
A:HIS196
|
4.2
|
20.5
|
1.0
|
ND1
|
A:HIS168
|
4.2
|
28.6
|
1.0
|
CE1
|
A:PHE185
|
4.2
|
24.0
|
1.0
|
CG
|
A:HIS183
|
4.2
|
26.3
|
1.0
|
CD2
|
A:HIS196
|
4.2
|
21.4
|
1.0
|
OG
|
A:SER172
|
4.4
|
31.1
|
1.0
|
CE2
|
A:PHE174
|
4.4
|
23.4
|
1.0
|
CB
|
A:ASP170
|
4.5
|
36.2
|
1.0
|
CZ
|
A:PHE174
|
4.7
|
21.7
|
1.0
|
HD1
|
A:HIS183
|
4.7
|
0.0
|
1.0
|
H2
|
A:HOH308
|
4.7
|
0.0
|
1.0
|
H1
|
A:HOH308
|
4.7
|
0.0
|
1.0
|
H
|
A:SER172
|
4.9
|
0.0
|
1.0
|
CB
|
A:SER172
|
4.9
|
32.5
|
1.0
|
CA
|
A:HIS196
|
4.9
|
21.4
|
1.0
|
C
|
A:SER172
|
5.0
|
34.0
|
1.0
|
O
|
A:HOH308
|
5.0
|
41.2
|
1.0
|
|
Zinc binding site 3 out
of 4 in 1cgf
Go back to
Zinc Binding Sites List in 1cgf
Zinc binding site 3 out
of 4 in the Crystal Structures of Recombinant 19-kDa Human Fibroblast Collagenase Complexed to Itself
Mono view
Stereo pair view
|
A full contact list of Zinc with other atoms in the Zn binding
site number 3 of Crystal Structures of Recombinant 19-kDa Human Fibroblast Collagenase Complexed to Itself within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
B:Zn301
b:21.8
occ:1.00
|
NE2
|
B:HIS218
|
2.0
|
15.1
|
1.0
|
NE2
|
B:HIS228
|
2.1
|
20.1
|
1.0
|
NE2
|
B:HIS222
|
2.1
|
17.1
|
1.0
|
CD2
|
B:HIS228
|
3.0
|
18.4
|
1.0
|
CD2
|
B:HIS218
|
3.0
|
12.8
|
1.0
|
CD2
|
B:HIS222
|
3.0
|
16.4
|
1.0
|
CE1
|
B:HIS218
|
3.1
|
14.0
|
1.0
|
CE1
|
B:HIS228
|
3.2
|
18.9
|
1.0
|
CE1
|
B:HIS222
|
3.2
|
14.2
|
1.0
|
ND1
|
B:HIS218
|
4.2
|
14.8
|
1.0
|
CG
|
B:HIS218
|
4.2
|
11.2
|
1.0
|
CG
|
B:HIS228
|
4.2
|
18.9
|
1.0
|
H1
|
B:HOH312
|
4.2
|
0.0
|
1.0
|
O
|
B:HOH396
|
4.2
|
42.9
|
1.0
|
CG
|
B:HIS222
|
4.2
|
15.7
|
1.0
|
ND1
|
B:HIS228
|
4.2
|
19.3
|
1.0
|
OE1
|
B:GLU219
|
4.3
|
19.8
|
1.0
|
ND1
|
B:HIS222
|
4.3
|
14.9
|
1.0
|
H1
|
B:HOH396
|
4.3
|
0.0
|
1.0
|
O
|
B:HOH349
|
4.4
|
36.6
|
1.0
|
O
|
B:HOH357
|
4.4
|
38.7
|
1.0
|
OE2
|
B:GLU219
|
4.4
|
21.0
|
1.0
|
H2
|
B:HOH396
|
4.4
|
0.0
|
1.0
|
O
|
B:HOH312
|
4.4
|
24.2
|
1.0
|
H2
|
B:HOH357
|
4.6
|
0.0
|
1.0
|
CD
|
B:GLU219
|
4.7
|
17.0
|
1.0
|
H1
|
B:HOH349
|
4.7
|
0.0
|
1.0
|
H2
|
B:HOH349
|
4.9
|
0.0
|
1.0
|
O
|
B:HOH419
|
5.0
|
36.6
|
1.0
|
|
Zinc binding site 4 out
of 4 in 1cgf
Go back to
Zinc Binding Sites List in 1cgf
Zinc binding site 4 out
of 4 in the Crystal Structures of Recombinant 19-kDa Human Fibroblast Collagenase Complexed to Itself
Mono view
Stereo pair view
|
A full contact list of Zinc with other atoms in the Zn binding
site number 4 of Crystal Structures of Recombinant 19-kDa Human Fibroblast Collagenase Complexed to Itself within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
B:Zn302
b:17.4
occ:1.00
|
ND1
|
B:HIS196
|
2.0
|
12.5
|
1.0
|
OD2
|
B:ASP170
|
2.1
|
17.4
|
1.0
|
NE2
|
B:HIS183
|
2.1
|
17.5
|
1.0
|
NE2
|
B:HIS168
|
2.2
|
20.7
|
1.0
|
CE1
|
B:HIS196
|
2.8
|
12.6
|
1.0
|
CG
|
B:ASP170
|
2.9
|
20.4
|
1.0
|
CE1
|
B:HIS183
|
3.0
|
16.9
|
1.0
|
OD1
|
B:ASP170
|
3.1
|
19.8
|
1.0
|
CD2
|
B:HIS168
|
3.1
|
19.1
|
1.0
|
CE1
|
B:HIS168
|
3.2
|
19.2
|
1.0
|
CG
|
B:HIS196
|
3.2
|
12.2
|
1.0
|
CD2
|
B:HIS183
|
3.2
|
15.9
|
1.0
|
CB
|
B:HIS196
|
3.7
|
10.7
|
1.0
|
NE2
|
B:HIS196
|
4.0
|
13.4
|
1.0
|
O
|
B:SER172
|
4.1
|
20.1
|
1.0
|
CB
|
B:ASP170
|
4.1
|
21.7
|
1.0
|
CZ
|
B:PHE185
|
4.2
|
20.2
|
1.0
|
CE1
|
B:PHE185
|
4.2
|
19.2
|
1.0
|
CD2
|
B:HIS196
|
4.2
|
11.1
|
1.0
|
ND1
|
B:HIS183
|
4.2
|
15.7
|
1.0
|
ND1
|
B:HIS168
|
4.3
|
18.3
|
1.0
|
CG
|
B:HIS168
|
4.3
|
19.9
|
1.0
|
CG
|
B:HIS183
|
4.3
|
16.4
|
1.0
|
H
|
B:SER172
|
4.7
|
0.0
|
1.0
|
CE2
|
B:PHE174
|
4.7
|
15.4
|
1.0
|
CB
|
B:SER172
|
4.8
|
19.7
|
1.0
|
CZ
|
B:PHE174
|
4.8
|
16.6
|
1.0
|
C
|
B:SER172
|
4.9
|
21.6
|
1.0
|
HE2
|
B:HIS196
|
4.9
|
0.0
|
1.0
|
|
Reference:
B.Lovejoy,
A.M.Hassell,
M.A.Luther,
D.Weigl,
S.R.Jordan.
Crystal Structures of Recombinant 19-kDa Human Fibroblast Collagenase Complexed to Itself. Biochemistry V. 33 8207 1994.
ISSN: ISSN 0006-2960
PubMed: 8031754
DOI: 10.1021/BI00193A006
Page generated: Sat Oct 12 23:05:24 2024
|