Zinc in PDB 9vck: Cryo-Em Structure of Sars-Cov-2 NSP10/NSP14:Rna:Smp Complex
Enzymatic activity of Cryo-Em Structure of Sars-Cov-2 NSP10/NSP14:Rna:Smp Complex
All present enzymatic activity of Cryo-Em Structure of Sars-Cov-2 NSP10/NSP14:Rna:Smp Complex:
2.1.1.56;
Other elements in 9vck:
The structure of Cryo-Em Structure of Sars-Cov-2 NSP10/NSP14:Rna:Smp Complex also contains other interesting chemical elements:
Zinc Binding Sites:
The binding sites of Zinc atom in the Cryo-Em Structure of Sars-Cov-2 NSP10/NSP14:Rna:Smp Complex
(pdb code 9vck). This binding sites where shown within
5.0 Angstroms radius around Zinc atom.
In total 5 binding sites of Zinc where determined in the
Cryo-Em Structure of Sars-Cov-2 NSP10/NSP14:Rna:Smp Complex, PDB code: 9vck:
Jump to Zinc binding site number:
1;
2;
3;
4;
5;
Zinc binding site 1 out
of 5 in 9vck
Go back to
Zinc Binding Sites List in 9vck
Zinc binding site 1 out
of 5 in the Cryo-Em Structure of Sars-Cov-2 NSP10/NSP14:Rna:Smp Complex
 Mono view
 Stereo pair view
|
A full contact list of Zinc with other atoms in the Zn binding
site number 1 of Cryo-Em Structure of Sars-Cov-2 NSP10/NSP14:Rna:Smp Complex within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Zn201
b:113.0
occ:1.00
|
NE2
|
A:HIS83
|
2.0
|
73.4
|
1.0
|
SG
|
A:CYS90
|
2.3
|
85.6
|
1.0
|
SG
|
A:CYS74
|
2.3
|
77.9
|
1.0
|
SG
|
A:CYS77
|
2.4
|
75.1
|
1.0
|
CB
|
A:CYS90
|
2.5
|
85.6
|
1.0
|
CD2
|
A:HIS83
|
2.5
|
73.4
|
1.0
|
CE1
|
A:HIS83
|
3.2
|
73.4
|
1.0
|
CB
|
A:CYS74
|
3.3
|
77.9
|
1.0
|
CA
|
A:CYS90
|
3.6
|
85.6
|
1.0
|
CG
|
A:HIS83
|
3.8
|
73.4
|
1.0
|
CB
|
A:CYS77
|
3.8
|
75.1
|
1.0
|
ND1
|
A:HIS83
|
4.1
|
73.4
|
1.0
|
N
|
A:CYS77
|
4.1
|
75.1
|
1.0
|
CA
|
A:CYS77
|
4.5
|
75.1
|
1.0
|
N
|
A:CYS90
|
4.5
|
85.6
|
1.0
|
CB
|
A:TYR76
|
4.6
|
73.2
|
1.0
|
C
|
A:CYS90
|
4.6
|
85.6
|
1.0
|
CA
|
A:CYS74
|
4.7
|
77.9
|
1.0
|
CD2
|
A:TYR76
|
5.0
|
73.2
|
1.0
|
|
Zinc binding site 2 out
of 5 in 9vck
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Zinc Binding Sites List in 9vck
Zinc binding site 2 out
of 5 in the Cryo-Em Structure of Sars-Cov-2 NSP10/NSP14:Rna:Smp Complex
 Mono view
 Stereo pair view
|
A full contact list of Zinc with other atoms in the Zn binding
site number 2 of Cryo-Em Structure of Sars-Cov-2 NSP10/NSP14:Rna:Smp Complex within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Zn202
b:145.3
occ:1.00
|
NZ
|
A:LYS124
|
2.1
|
112.5
|
1.0
|
SG
|
A:CYS128
|
2.3
|
118.0
|
1.0
|
CD
|
A:LYS124
|
2.4
|
112.5
|
1.0
|
CE
|
A:LYS124
|
2.5
|
112.5
|
1.0
|
SG
|
A:CYS130
|
2.9
|
116.7
|
1.0
|
CB
|
A:CYS130
|
3.0
|
116.7
|
1.0
|
O
|
A:CYS128
|
3.3
|
118.0
|
1.0
|
CB
|
A:CYS128
|
3.7
|
118.0
|
1.0
|
SG
|
A:CYS117
|
3.7
|
118.0
|
1.0
|
CG
|
A:LYS124
|
3.8
|
112.5
|
1.0
|
N
|
A:CYS130
|
4.1
|
116.7
|
1.0
|
CA
|
A:CYS130
|
4.1
|
116.7
|
1.0
|
C
|
A:CYS128
|
4.2
|
118.0
|
1.0
|
CB
|
A:CYS117
|
4.4
|
118.0
|
1.0
|
CB
|
A:LYS124
|
4.5
|
112.5
|
1.0
|
CA
|
A:CYS128
|
4.5
|
118.0
|
1.0
|
CA
|
A:LYS124
|
4.8
|
112.5
|
1.0
|
C
|
A:CYS130
|
4.9
|
116.7
|
1.0
|
|
Zinc binding site 3 out
of 5 in 9vck
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Zinc Binding Sites List in 9vck
Zinc binding site 3 out
of 5 in the Cryo-Em Structure of Sars-Cov-2 NSP10/NSP14:Rna:Smp Complex
 Mono view
 Stereo pair view
|
A full contact list of Zinc with other atoms in the Zn binding
site number 3 of Cryo-Em Structure of Sars-Cov-2 NSP10/NSP14:Rna:Smp Complex within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
B:Zn603
b:113.2
occ:1.00
|
ND1
|
B:HIS229
|
2.0
|
81.5
|
1.0
|
SG
|
B:CYS207
|
2.3
|
86.9
|
1.0
|
SG
|
B:CYS226
|
2.4
|
86.9
|
1.0
|
CB
|
B:CYS210
|
2.4
|
92.8
|
1.0
|
SG
|
B:CYS210
|
2.6
|
92.8
|
1.0
|
CG
|
B:HIS229
|
2.8
|
81.5
|
1.0
|
CE1
|
B:HIS229
|
2.9
|
81.5
|
1.0
|
CB
|
B:HIS229
|
3.2
|
81.5
|
1.0
|
CB
|
B:CYS207
|
3.3
|
86.9
|
1.0
|
CD2
|
B:HIS229
|
3.8
|
81.5
|
1.0
|
NE2
|
B:HIS229
|
3.8
|
81.5
|
1.0
|
CA
|
B:CYS210
|
3.8
|
92.8
|
1.0
|
CB
|
B:CYS226
|
3.8
|
86.9
|
1.0
|
O
|
B:LEU209
|
3.9
|
88.9
|
1.0
|
N
|
B:CYS226
|
4.2
|
86.9
|
1.0
|
O
|
B:CYS208
|
4.4
|
87.5
|
1.0
|
N
|
B:CYS210
|
4.4
|
92.8
|
1.0
|
C
|
B:LEU209
|
4.4
|
88.9
|
1.0
|
CA
|
B:HIS229
|
4.5
|
81.5
|
1.0
|
N
|
B:HIS229
|
4.5
|
81.5
|
1.0
|
CA
|
B:CYS226
|
4.5
|
86.9
|
1.0
|
CA
|
B:CYS207
|
4.6
|
86.9
|
1.0
|
O
|
B:CYS207
|
4.7
|
86.9
|
1.0
|
NH1
|
B:ARG212
|
4.7
|
88.2
|
1.0
|
C
|
B:CYS207
|
4.8
|
86.9
|
1.0
|
C
|
B:CYS210
|
4.9
|
92.8
|
1.0
|
N
|
B:ASP211
|
5.0
|
88.3
|
1.0
|
C
|
B:CYS226
|
5.0
|
86.9
|
1.0
|
|
Zinc binding site 4 out
of 5 in 9vck
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Zinc Binding Sites List in 9vck
Zinc binding site 4 out
of 5 in the Cryo-Em Structure of Sars-Cov-2 NSP10/NSP14:Rna:Smp Complex
 Mono view
 Stereo pair view
|
A full contact list of Zinc with other atoms in the Zn binding
site number 4 of Cryo-Em Structure of Sars-Cov-2 NSP10/NSP14:Rna:Smp Complex within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
B:Zn604
b:121.2
occ:1.00
|
NE2
|
B:HIS257
|
2.2
|
94.0
|
1.0
|
ND1
|
B:HIS264
|
2.2
|
90.2
|
1.0
|
SG
|
B:CYS279
|
2.3
|
86.0
|
1.0
|
CB
|
B:CYS261
|
2.7
|
101.4
|
1.0
|
CD2
|
B:HIS257
|
2.9
|
94.0
|
1.0
|
CE1
|
B:HIS264
|
3.0
|
90.2
|
1.0
|
SG
|
B:CYS261
|
3.0
|
101.4
|
1.0
|
CG
|
B:HIS264
|
3.3
|
90.2
|
1.0
|
CE1
|
B:HIS257
|
3.3
|
94.0
|
1.0
|
CB
|
B:CYS279
|
3.3
|
86.0
|
1.0
|
CB
|
B:HIS264
|
3.8
|
90.2
|
1.0
|
CA
|
B:CYS261
|
4.1
|
101.4
|
1.0
|
NE2
|
B:HIS264
|
4.1
|
90.2
|
1.0
|
CG
|
B:HIS257
|
4.2
|
94.0
|
1.0
|
ND1
|
B:HIS257
|
4.3
|
94.0
|
1.0
|
CD2
|
B:HIS264
|
4.3
|
90.2
|
1.0
|
N
|
B:CYS261
|
4.7
|
101.4
|
1.0
|
SG
|
B:CYS414
|
4.7
|
169.4
|
1.0
|
CA
|
B:CYS279
|
4.8
|
86.0
|
1.0
|
N
|
B:ASP415
|
4.9
|
186.0
|
1.0
|
N
|
B:HIS264
|
4.9
|
90.2
|
1.0
|
|
Zinc binding site 5 out
of 5 in 9vck
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Zinc Binding Sites List in 9vck
Zinc binding site 5 out
of 5 in the Cryo-Em Structure of Sars-Cov-2 NSP10/NSP14:Rna:Smp Complex
 Mono view
 Stereo pair view
|
A full contact list of Zinc with other atoms in the Zn binding
site number 5 of Cryo-Em Structure of Sars-Cov-2 NSP10/NSP14:Rna:Smp Complex within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
B:Zn605
b:275.7
occ:1.00
|
CB
|
B:CYS452
|
2.2
|
272.4
|
1.0
|
ND1
|
B:HIS487
|
2.3
|
223.6
|
1.0
|
SG
|
B:CYS452
|
2.3
|
272.4
|
1.0
|
SG
|
B:CYS484
|
2.3
|
256.4
|
1.0
|
CE1
|
B:HIS487
|
2.5
|
223.6
|
1.0
|
CA
|
B:CYS452
|
3.0
|
272.4
|
1.0
|
CG
|
B:HIS487
|
3.4
|
223.6
|
1.0
|
CB
|
B:CYS477
|
3.5
|
281.1
|
1.0
|
CB
|
B:CYS484
|
3.6
|
256.4
|
1.0
|
NE2
|
B:HIS487
|
3.6
|
223.6
|
1.0
|
SG
|
B:CYS477
|
3.9
|
281.1
|
1.0
|
N
|
B:CYS452
|
4.0
|
272.4
|
1.0
|
CD2
|
B:HIS487
|
4.1
|
223.6
|
1.0
|
CB
|
B:HIS486
|
4.1
|
241.8
|
1.0
|
C
|
B:CYS452
|
4.1
|
272.4
|
1.0
|
CB
|
B:HIS487
|
4.2
|
223.6
|
1.0
|
CD2
|
B:HIS486
|
4.3
|
241.8
|
1.0
|
N
|
B:GLU453
|
4.4
|
278.6
|
1.0
|
CG
|
B:HIS486
|
4.5
|
241.8
|
1.0
|
C
|
B:HIS486
|
4.5
|
241.8
|
1.0
|
N
|
B:HIS487
|
4.5
|
223.6
|
1.0
|
CA
|
B:HIS486
|
4.8
|
241.8
|
1.0
|
CA
|
B:CYS477
|
4.8
|
281.1
|
1.0
|
O
|
B:GLU453
|
4.8
|
278.6
|
1.0
|
O
|
B:HIS486
|
4.8
|
241.8
|
1.0
|
CA
|
B:CYS484
|
4.9
|
256.4
|
1.0
|
N
|
B:CYS477
|
4.9
|
281.1
|
1.0
|
C
|
B:PRO451
|
4.9
|
252.4
|
1.0
|
|
Reference:
J.Wang,
Y.Pan,
Y.Liu,
B.Huang,
G.Jin,
L.Zhang,
F.Zhou,
X.Chang,
Y.Huang,
L.Yan,
Y.Dong,
Z.Rao,
D.Liu,
Z.Lou.
Structural Basis and Rational Design of Nucleotide Analogue Inhibitor Evading the Sars-Cov-2 Proofreading Enzyme. J.Am.Chem.Soc. V. 147 21896 2025.
ISSN: ESSN 1520-5126
PubMed: 40509573
DOI: 10.1021/JACS.5C05014
Page generated: Fri Aug 22 18:59:33 2025
|