Zinc in PDB 9j3p: Human Pigment Epithelium-Derived Factor with Zinc Ions Crystallized in P2(1)2(1)2(1) Space Group
Protein crystallography data
The structure of Human Pigment Epithelium-Derived Factor with Zinc Ions Crystallized in P2(1)2(1)2(1) Space Group, PDB code: 9j3p
was solved by
A.S.Belousov,
D.V.Chistyakov,
V.E.Baksheeva,
T.K.Bulgakov,
S.S.Bukhdruker,
A.A.Zamyatnin,
D.V.Zinchenko,
P.O.Tsvetkov,
S.E.Permyakov,
E.Y.Zernii,
V.I.Borshchevskiy,
with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:
Resolution Low / High (Å)
|
19.95 /
2.10
|
Space group
|
P 21 21 21
|
Cell size a, b, c (Å), α, β, γ (°)
|
74.234,
131.567,
227.831,
90,
90,
90
|
R / Rfree (%)
|
21.7 /
25.1
|
Zinc Binding Sites:
The binding sites of Zinc atom in the Human Pigment Epithelium-Derived Factor with Zinc Ions Crystallized in P2(1)2(1)2(1) Space Group
(pdb code 9j3p). This binding sites where shown within
5.0 Angstroms radius around Zinc atom.
In total 7 binding sites of Zinc where determined in the
Human Pigment Epithelium-Derived Factor with Zinc Ions Crystallized in P2(1)2(1)2(1) Space Group, PDB code: 9j3p:
Jump to Zinc binding site number:
1;
2;
3;
4;
5;
6;
7;
Zinc binding site 1 out
of 7 in 9j3p
Go back to
Zinc Binding Sites List in 9j3p
Zinc binding site 1 out
of 7 in the Human Pigment Epithelium-Derived Factor with Zinc Ions Crystallized in P2(1)2(1)2(1) Space Group
 Mono view
 Stereo pair view
|
A full contact list of Zinc with other atoms in the Zn binding
site number 1 of Human Pigment Epithelium-Derived Factor with Zinc Ions Crystallized in P2(1)2(1)2(1) Space Group within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Zn501
b:59.2
occ:0.96
|
ND1
|
A:HIS381
|
2.0
|
55.6
|
1.0
|
O
|
A:HOH680
|
2.2
|
63.1
|
1.0
|
HB3
|
A:HIS381
|
2.8
|
57.4
|
1.0
|
CG
|
A:HIS381
|
3.0
|
60.1
|
1.0
|
CE1
|
A:HIS381
|
3.0
|
56.3
|
1.0
|
HA
|
A:HIS381
|
3.2
|
56.5
|
1.0
|
HE1
|
A:HIS381
|
3.2
|
56.3
|
1.0
|
CB
|
A:HIS381
|
3.2
|
57.4
|
1.0
|
CA
|
A:HIS381
|
3.7
|
56.5
|
1.0
|
O
|
A:HIS381
|
3.9
|
56.7
|
1.0
|
CD2
|
A:HIS381
|
4.1
|
57.0
|
1.0
|
NE2
|
A:HIS381
|
4.1
|
57.1
|
1.0
|
HB2
|
A:HIS381
|
4.1
|
57.4
|
1.0
|
C
|
A:HIS381
|
4.3
|
61.6
|
1.0
|
HE2
|
A:HIS381
|
4.9
|
57.1
|
1.0
|
HD2
|
A:HIS381
|
4.9
|
57.0
|
1.0
|
N
|
A:HIS381
|
5.0
|
59.5
|
1.0
|
|
Zinc binding site 2 out
of 7 in 9j3p
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Zinc Binding Sites List in 9j3p
Zinc binding site 2 out
of 7 in the Human Pigment Epithelium-Derived Factor with Zinc Ions Crystallized in P2(1)2(1)2(1) Space Group
 Mono view
 Stereo pair view
|
A full contact list of Zinc with other atoms in the Zn binding
site number 2 of Human Pigment Epithelium-Derived Factor with Zinc Ions Crystallized in P2(1)2(1)2(1) Space Group within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
C:Zn501
b:75.7
occ:1.00
|
OD2
|
C:ASP44
|
2.0
|
70.7
|
1.0
|
NE2
|
C:HIS299
|
2.0
|
72.9
|
1.0
|
OE2
|
B:GLU236
|
2.0
|
83.4
|
1.0
|
OE1
|
B:GLU236
|
2.6
|
80.2
|
1.0
|
OD1
|
C:ASP44
|
2.6
|
74.5
|
1.0
|
CG
|
C:ASP44
|
2.6
|
72.8
|
1.0
|
CD
|
B:GLU236
|
2.6
|
81.3
|
1.0
|
CD2
|
C:HIS299
|
2.8
|
71.8
|
1.0
|
HD2
|
C:HIS299
|
2.9
|
71.7
|
1.0
|
HD3
|
C:ARG303
|
3.1
|
74.8
|
1.0
|
CE1
|
C:HIS299
|
3.1
|
70.9
|
1.0
|
HD2
|
C:ARG303
|
3.2
|
74.8
|
1.0
|
HE1
|
C:HIS299
|
3.4
|
70.9
|
1.0
|
CD
|
C:ARG303
|
3.6
|
74.8
|
1.0
|
HB3
|
C:PHE46
|
3.7
|
67.8
|
1.0
|
HB2
|
C:PHE46
|
3.8
|
67.8
|
1.0
|
HD2
|
C:PRO45
|
3.9
|
86.1
|
1.0
|
H
|
C:PHE46
|
3.9
|
72.5
|
1.0
|
CG
|
C:HIS299
|
4.1
|
70.7
|
1.0
|
CB
|
C:ASP44
|
4.1
|
73.9
|
1.0
|
CG
|
B:GLU236
|
4.1
|
77.2
|
1.0
|
ND1
|
C:HIS299
|
4.1
|
70.8
|
1.0
|
NE
|
C:ARG303
|
4.1
|
79.3
|
1.0
|
HE
|
C:ARG303
|
4.2
|
79.3
|
1.0
|
CB
|
C:PHE46
|
4.2
|
67.8
|
1.0
|
HB2
|
C:ASP44
|
4.4
|
73.9
|
1.0
|
HG2
|
B:GLU236
|
4.4
|
77.2
|
1.0
|
HG3
|
B:GLU236
|
4.5
|
77.2
|
1.0
|
HA
|
C:ASP44
|
4.6
|
77.8
|
1.0
|
HB3
|
C:ASP44
|
4.6
|
73.9
|
1.0
|
N
|
C:PHE46
|
4.7
|
72.6
|
1.0
|
CD
|
C:PRO45
|
4.7
|
86.1
|
1.0
|
CA
|
C:ASP44
|
4.8
|
77.8
|
1.0
|
HG3
|
C:ARG303
|
4.8
|
68.5
|
1.0
|
HB3
|
B:GLU236
|
4.8
|
78.8
|
1.0
|
CG
|
C:ARG303
|
4.9
|
68.5
|
1.0
|
HD1
|
C:HIS299
|
4.9
|
70.8
|
1.0
|
N
|
C:PRO45
|
5.0
|
82.3
|
1.0
|
H
|
C:PHE47
|
5.0
|
66.6
|
1.0
|
CZ
|
C:ARG303
|
5.0
|
79.2
|
1.0
|
|
Zinc binding site 3 out
of 7 in 9j3p
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Zinc Binding Sites List in 9j3p
Zinc binding site 3 out
of 7 in the Human Pigment Epithelium-Derived Factor with Zinc Ions Crystallized in P2(1)2(1)2(1) Space Group
 Mono view
 Stereo pair view
|
A full contact list of Zinc with other atoms in the Zn binding
site number 3 of Human Pigment Epithelium-Derived Factor with Zinc Ions Crystallized in P2(1)2(1)2(1) Space Group within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
C:Zn502
b:73.6
occ:0.72
|
NE2
|
C:HIS381
|
2.0
|
77.4
|
1.0
|
HE1
|
C:HIS381
|
2.4
|
77.3
|
1.0
|
CE1
|
C:HIS381
|
2.4
|
77.3
|
1.0
|
CD2
|
C:HIS381
|
3.2
|
75.8
|
1.0
|
ND1
|
C:HIS381
|
3.6
|
77.5
|
1.0
|
HD2
|
C:HIS381
|
3.7
|
75.8
|
1.0
|
CG
|
C:HIS381
|
4.0
|
74.3
|
1.0
|
HD1
|
C:HIS381
|
4.2
|
77.5
|
1.0
|
HD23
|
C:LEU382
|
4.9
|
81.9
|
1.0
|
|
Zinc binding site 4 out
of 7 in 9j3p
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Zinc Binding Sites List in 9j3p
Zinc binding site 4 out
of 7 in the Human Pigment Epithelium-Derived Factor with Zinc Ions Crystallized in P2(1)2(1)2(1) Space Group
 Mono view
 Stereo pair view
|
A full contact list of Zinc with other atoms in the Zn binding
site number 4 of Human Pigment Epithelium-Derived Factor with Zinc Ions Crystallized in P2(1)2(1)2(1) Space Group within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
D:Zn501
b:71.3
occ:1.00
|
ND1
|
D:HIS381
|
2.0
|
75.7
|
1.0
|
ND1
|
B:HIS381
|
2.2
|
69.7
|
1.0
|
HA
|
D:HIS381
|
2.9
|
88.8
|
1.0
|
CG
|
D:HIS381
|
2.9
|
78.2
|
1.0
|
CE1
|
D:HIS381
|
2.9
|
72.5
|
1.0
|
HB3
|
D:HIS381
|
3.0
|
80.4
|
1.0
|
CE1
|
B:HIS381
|
3.1
|
70.3
|
1.0
|
HE1
|
D:HIS381
|
3.2
|
72.5
|
1.0
|
HE1
|
B:HIS381
|
3.2
|
70.3
|
1.0
|
CG
|
B:HIS381
|
3.2
|
69.2
|
1.0
|
CB
|
D:HIS381
|
3.3
|
80.4
|
1.0
|
HB2
|
B:HIS381
|
3.4
|
66.4
|
1.0
|
HB3
|
B:HIS381
|
3.4
|
66.4
|
1.0
|
CA
|
D:HIS381
|
3.5
|
88.8
|
1.0
|
CB
|
B:HIS381
|
3.6
|
66.4
|
1.0
|
O
|
D:ALA380
|
3.9
|
84.7
|
1.0
|
NE2
|
D:HIS381
|
4.0
|
78.2
|
1.0
|
CD2
|
D:HIS381
|
4.0
|
87.9
|
1.0
|
NE2
|
B:HIS381
|
4.2
|
69.3
|
1.0
|
H
|
D:LEU382
|
4.2
|
96.2
|
0.5
|
H
|
D:LEU382
|
4.2
|
96.2
|
0.5
|
HB2
|
D:HIS381
|
4.2
|
80.4
|
1.0
|
CD2
|
B:HIS381
|
4.3
|
67.4
|
1.0
|
N
|
D:HIS381
|
4.5
|
99.7
|
1.0
|
C
|
D:HIS381
|
4.6
|
90.5
|
1.0
|
N
|
D:LEU382
|
4.7
|
96.2
|
1.0
|
C
|
D:ALA380
|
4.7
|
90.7
|
1.0
|
HE2
|
D:HIS381
|
4.8
|
78.2
|
1.0
|
HD2
|
D:HIS381
|
4.9
|
87.9
|
1.0
|
HE2
|
B:HIS381
|
4.9
|
69.3
|
1.0
|
HG
|
B:LEU382
|
5.0
|
62.1
|
1.0
|
|
Zinc binding site 5 out
of 7 in 9j3p
Go back to
Zinc Binding Sites List in 9j3p
Zinc binding site 5 out
of 7 in the Human Pigment Epithelium-Derived Factor with Zinc Ions Crystallized in P2(1)2(1)2(1) Space Group
 Mono view
 Stereo pair view
|
A full contact list of Zinc with other atoms in the Zn binding
site number 5 of Human Pigment Epithelium-Derived Factor with Zinc Ions Crystallized in P2(1)2(1)2(1) Space Group within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
E:Zn501
b:74.5
occ:1.00
|
NE2
|
E:HIS299
|
2.1
|
76.4
|
1.0
|
OD1
|
E:ASP44
|
2.1
|
75.6
|
1.0
|
O
|
E:HOH615
|
2.2
|
75.3
|
1.0
|
OD2
|
E:ASP44
|
2.3
|
79.7
|
1.0
|
CG
|
E:ASP44
|
2.5
|
74.1
|
1.0
|
HD2
|
E:HIS299
|
2.6
|
78.8
|
1.0
|
CD2
|
E:HIS299
|
2.7
|
78.8
|
1.0
|
CE1
|
E:HIS299
|
3.3
|
73.5
|
1.0
|
HG3
|
E:ARG303
|
3.6
|
68.6
|
1.0
|
HB3
|
E:PHE46
|
3.7
|
67.6
|
1.0
|
HD2
|
E:PRO45
|
3.7
|
81.2
|
1.0
|
HE1
|
E:HIS299
|
3.8
|
73.5
|
1.0
|
H
|
E:PHE46
|
3.8
|
72.6
|
1.0
|
HB2
|
E:PHE46
|
3.8
|
67.6
|
1.0
|
CG
|
E:HIS299
|
4.0
|
77.4
|
1.0
|
CB
|
E:ASP44
|
4.0
|
77.0
|
1.0
|
HD3
|
E:ARG303
|
4.1
|
66.9
|
1.0
|
HE
|
E:ARG303
|
4.1
|
67.9
|
1.0
|
CB
|
E:PHE46
|
4.2
|
67.6
|
1.0
|
ND1
|
E:HIS299
|
4.3
|
74.7
|
1.0
|
NE
|
E:ARG303
|
4.3
|
67.9
|
1.0
|
CG
|
E:ARG303
|
4.4
|
68.6
|
1.0
|
HB2
|
E:ASP44
|
4.5
|
77.0
|
1.0
|
CD
|
E:ARG303
|
4.5
|
66.9
|
1.0
|
HA
|
E:ASP44
|
4.5
|
82.3
|
1.0
|
HB3
|
E:ASP44
|
4.5
|
77.0
|
1.0
|
N
|
E:PHE46
|
4.6
|
72.6
|
1.0
|
CD
|
E:PRO45
|
4.6
|
81.2
|
1.0
|
CA
|
E:ASP44
|
4.7
|
82.3
|
1.0
|
HG2
|
E:ARG303
|
4.8
|
68.6
|
1.0
|
HE2
|
E:PHE47
|
4.9
|
68.7
|
1.0
|
N
|
E:PRO45
|
4.9
|
79.4
|
1.0
|
C
|
E:ASP44
|
5.0
|
76.7
|
1.0
|
|
Zinc binding site 6 out
of 7 in 9j3p
Go back to
Zinc Binding Sites List in 9j3p
Zinc binding site 6 out
of 7 in the Human Pigment Epithelium-Derived Factor with Zinc Ions Crystallized in P2(1)2(1)2(1) Space Group
 Mono view
 Stereo pair view
|
A full contact list of Zinc with other atoms in the Zn binding
site number 6 of Human Pigment Epithelium-Derived Factor with Zinc Ions Crystallized in P2(1)2(1)2(1) Space Group within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
E:Zn502
b:85.8
occ:0.52
|
NE2
|
E:HIS381
|
2.1
|
92.3
|
1.0
|
O
|
E:HOH640
|
2.2
|
82.9
|
1.0
|
HD2
|
E:HIS381
|
2.8
|
85.3
|
1.0
|
CD2
|
E:HIS381
|
2.8
|
85.3
|
1.0
|
CE1
|
E:HIS381
|
3.3
|
85.5
|
1.0
|
HE1
|
E:HIS381
|
3.6
|
85.5
|
1.0
|
CG
|
E:HIS381
|
4.0
|
83.8
|
1.0
|
ND1
|
E:HIS381
|
4.2
|
84.5
|
1.0
|
|
Zinc binding site 7 out
of 7 in 9j3p
Go back to
Zinc Binding Sites List in 9j3p
Zinc binding site 7 out
of 7 in the Human Pigment Epithelium-Derived Factor with Zinc Ions Crystallized in P2(1)2(1)2(1) Space Group
 Mono view
 Stereo pair view
|
A full contact list of Zinc with other atoms in the Zn binding
site number 7 of Human Pigment Epithelium-Derived Factor with Zinc Ions Crystallized in P2(1)2(1)2(1) Space Group within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
E:Zn503
b:82.1
occ:0.57
|
ND1
|
E:HIS105
|
2.1
|
72.5
|
1.0
|
O
|
E:HOH651
|
2.2
|
79.8
|
1.0
|
O
|
E:HOH661
|
2.2
|
94.2
|
1.0
|
CE1
|
E:HIS105
|
3.0
|
76.5
|
1.0
|
HE1
|
E:HIS105
|
3.1
|
76.5
|
1.0
|
CG
|
E:HIS105
|
3.1
|
67.3
|
1.0
|
HB3
|
E:HIS105
|
3.3
|
65.1
|
1.0
|
CB
|
E:HIS105
|
3.6
|
65.1
|
1.0
|
HB2
|
E:HIS105
|
3.7
|
65.1
|
1.0
|
NE2
|
E:HIS105
|
4.1
|
74.2
|
1.0
|
HG2
|
E:GLU101
|
4.2
|
72.9
|
1.0
|
CD2
|
E:HIS105
|
4.2
|
68.1
|
1.0
|
HB3
|
E:TYR110
|
4.3
|
65.3
|
1.0
|
HD2
|
E:TYR110
|
4.3
|
65.0
|
1.0
|
HB2
|
E:TYR110
|
4.6
|
65.3
|
1.0
|
HA
|
E:SER102
|
4.7
|
70.7
|
1.0
|
OE1
|
E:GLU101
|
4.9
|
75.8
|
1.0
|
HE2
|
E:HIS105
|
4.9
|
74.2
|
1.0
|
CB
|
E:TYR110
|
4.9
|
65.3
|
1.0
|
|
Reference:
D.V.Chistyakov,
M.P.Shevelyova,
E.N.Iomdina,
V.E.Baksheeva,
L.Wu,
N.G.Shebardina,
A.M.Moysenovich,
T.K.Bulgakov,
S.Y.Petrov,
V.V.Tiulina,
E.I.Pogodina,
O.S.Gancharova,
A.S.Belousov,
O.M.Filippova,
A.V.Baldin,
S.V.Goriainov,
A.I.Nikolskaya,
A.O.Zalevsky,
A.A.Deviatkin,
A.A.Vologzhannikova,
N.V.Gorokhovets,
E.A.Litus,
A.A.Zamyatnin,
S.V.Komarov,
F.Devred,
M.G.Sergeeva,
A.A.Zamyatnin Jr,
I.I.Senin,
D.V.Zinchenko,
P.O.Tsvetkov,
V.I.Borshchevskiy,
S.E.Permyakov,
E.Y.Zernii.
Zinc-Mediated Mechanism of Neurodegeneration in Glaucoma: A Role of Pigment Epithelium-Derived Factor To Be Published.
Page generated: Fri Aug 22 18:19:19 2025
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