Zinc in PDB 9b0z: Structure of Optineurin Bound to Hoip NZF1 Domain and M1-Linked Diubiquitin, Crystal Form 2

All present enzymatic activity of Structure of Optineurin Bound to Hoip NZF1 Domain and M1-Linked Diubiquitin, Crystal Form 2:
2.3.2.31;

The structure of Structure of Optineurin Bound to Hoip NZF1 Domain and M1-Linked Diubiquitin, Crystal Form 2, PDB code: 9b0z was solved by M.A.Michel, S.Scutts, D.Komander, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	44.18 / 2.41
Space group	C 1 2 1
Cell size a, b, c (Å), α, β, γ (°)	247.573, 72.313, 56.345, 90, 95.07, 90
R / Rfree (%)	22.1 / 27

The binding sites of Zinc atom in the Structure of Optineurin Bound to Hoip NZF1 Domain and M1-Linked Diubiquitin, Crystal Form 2 (pdb code 9b0z). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Structure of Optineurin Bound to Hoip NZF1 Domain and M1-Linked Diubiquitin, Crystal Form 2, PDB code: 9b0z:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Structure of Optineurin Bound to Hoip NZF1 Domain and M1-Linked Diubiquitin, Crystal Form 2


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Structure of Optineurin Bound to Hoip NZF1 Domain and M1-Linked Diubiquitin, Crystal Form 2 within 5.0Å range: probe atom residue distance (Å) B Occ F:Zn401 b:95.0 occ:1.00 SG F:CYS370 2.3 106.7 1.0 SG F:CYS359 2.3 164.6 1.0 SG F:CYS373 2.3 105.9 1.0 CG2 F:ILE372 3.0 119.2 1.0 CB F:CYS370 3.2 98.2 1.0 SG F:CYS356 3.3 118.8 1.0 OG F:SER358 3.3 131.8 1.0 CB F:CYS359 3.4 123.8 1.0 CB F:CYS356 3.6 123.2 1.0 CB F:CYS373 3.8 110.1 1.0 N F:CYS373 3.9 100.8 1.0 N F:CYS359 4.0 119.5 1.0 CA F:CYS359 4.3 125.9 1.0 CB F:ILE372 4.3 114.8 1.0 CA F:CYS373 4.5 103.4 1.0 CB F:PHE361 4.6 136.4 1.0 CA F:CYS370 4.7 95.7 1.0 CB F:SER358 4.7 118.5 1.0 N F:ILE372 4.8 110.5 1.0 C F:ILE372 4.9 103.0 1.0 CA F:ILE372 4.9 115.1 1.0

Zinc binding site 2 out of 2 in the Structure of Optineurin Bound to Hoip NZF1 Domain and M1-Linked Diubiquitin, Crystal Form 2


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Structure of Optineurin Bound to Hoip NZF1 Domain and M1-Linked Diubiquitin, Crystal Form 2 within 5.0Å range: probe atom residue distance (Å) B Occ E:Zn401 b:46.0 occ:1.00 SG E:CYS373 2.3 39.9 1.0 SG E:CYS370 2.3 48.5 1.0 SG E:CYS359 2.3 37.1 1.0 SG E:CYS356 2.3 46.8 1.0 CB E:CYS356 2.9 43.4 1.0 CB E:CYS370 3.0 55.4 1.0 CB E:CYS359 3.4 32.0 1.0 N E:CYS359 3.7 49.9 1.0 CB E:CYS373 3.8 39.2 1.0 N E:CYS373 3.9 46.0 1.0 CA E:CYS359 4.0 34.5 1.0 CA E:CYS356 4.4 52.9 1.0 CA E:CYS373 4.4 36.9 1.0 CA E:CYS370 4.5 38.1 1.0 C E:CYS359 4.6 27.3 1.0 CB E:ILE372 4.6 38.4 1.0 CB E:PHE361 4.8 40.3 1.0 N E:THR360 4.8 32.4 1.0 N E:PHE361 4.8 46.5 1.0 C E:SER358 4.8 39.2 1.0 CB E:SER358 4.8 38.9 1.0 N E:SER358 5.0 45.0 1.0 N E:GLU374 5.0 44.1 1.0 N E:ILE372 5.0 40.6 1.0 C E:CYS356 5.0 48.5 1.0 C E:ILE372 5.0 38.9 1.0

M.A.Michel, S.Scutts, D.Komander. Linkage and Substrate Specificity Conferred By Nzf Ubiquitin Binding Domains To Be Published.