Zinc in PDB 8uqc: Crystal Structure of RNF168 (Ring)-UBCH5C Fused to H2A-H2B Via A 20- Residue Linker (Crystallization Condition 2)

All present enzymatic activity of Crystal Structure of RNF168 (Ring)-UBCH5C Fused to H2A-H2B Via A 20- Residue Linker (Crystallization Condition 2):
2.3.2.23; 2.3.2.24; 2.3.2.27;

The structure of Crystal Structure of RNF168 (Ring)-UBCH5C Fused to H2A-H2B Via A 20- Residue Linker (Crystallization Condition 2), PDB code: 8uqc was solved by Q.Hu, M.V.Botuyan, G.Mer, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	43.94 / 2.61
Space group	P 32 2 1
Cell size a, b, c (Å), α, β, γ (°)	101.981, 101.981, 86.569, 90, 90, 120
R / Rfree (%)	21.9 / 25.2

The binding sites of Zinc atom in the Crystal Structure of RNF168 (Ring)-UBCH5C Fused to H2A-H2B Via A 20- Residue Linker (Crystallization Condition 2) (pdb code 8uqc). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Crystal Structure of RNF168 (Ring)-UBCH5C Fused to H2A-H2B Via A 20- Residue Linker (Crystallization Condition 2), PDB code: 8uqc:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Crystal Structure of RNF168 (Ring)-UBCH5C Fused to H2A-H2B Via A 20- Residue Linker (Crystallization Condition 2)


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Crystal Structure of RNF168 (Ring)-UBCH5C Fused to H2A-H2B Via A 20- Residue Linker (Crystallization Condition 2) within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn4001 b:45.4 occ:1.00 ND1 A:HIS33 2.1 42.8 1.0 SG A:CYS31 2.3 43.6 1.0 SG A:CYS54 2.3 47.9 1.0 SG A:CYS51 2.3 41.2 1.0 HB2 A:HIS33 2.7 53.0 1.0 HB2 A:CYS31 2.9 54.0 1.0 CE1 A:HIS33 3.0 44.7 1.0 CG A:HIS33 3.1 44.6 1.0 CB A:CYS31 3.1 45.0 1.0 CB A:CYS51 3.2 47.1 1.0 HB3 A:CYS51 3.2 56.6 1.0 H A:CYS54 3.2 52.6 1.0 HB2 A:CYS51 3.2 56.6 1.0 HE1 A:HIS33 3.2 53.7 1.0 CB A:HIS33 3.4 44.1 1.0 HB2 A:PHE53 3.5 50.4 1.0 HB3 A:CYS31 3.5 54.0 1.0 CB A:CYS54 3.7 45.2 1.0 HB3 A:CYS54 3.7 54.3 1.0 H A:HIS33 3.7 52.5 1.0 N A:CYS54 3.9 43.8 1.0 HB3 A:HIS33 3.9 53.0 1.0 HD12 A:LEU29 4.1 55.9 1.0 NE2 A:HIS33 4.2 43.1 1.0 CD2 A:HIS33 4.2 43.7 1.0 HG3 A:ARG56 4.2 69.1 1.0 HD2 A:PHE53 4.2 51.2 1.0 CA A:CYS54 4.4 46.6 1.0 H A:PHE53 4.4 51.1 1.0 HB2 A:ARG56 4.4 64.0 1.0 N A:HIS33 4.4 43.7 1.0 H A:ARG56 4.4 61.9 1.0 HB2 A:CYS54 4.4 54.3 1.0 CB A:PHE53 4.4 42.0 1.0 CA A:CYS31 4.5 43.5 1.0 CA A:HIS33 4.5 43.1 1.0 H A:ARG55 4.6 60.1 1.0 HB2 A:LEU29 4.6 56.1 1.0 CA A:CYS51 4.7 50.2 1.0 C A:CYS31 4.7 40.9 1.0 HD13 A:LEU29 4.7 55.9 1.0 O A:CYS31 4.7 42.2 1.0 H A:CYS31 4.8 54.1 1.0 HB3 A:PHE53 4.8 50.4 1.0 CD1 A:LEU29 4.9 46.5 1.0 C A:PHE53 4.9 44.0 1.0 HE2 A:HIS33 4.9 51.7 1.0 HA A:CYS51 4.9 60.2 1.0 C A:CYS54 5.0 49.2 1.0

Zinc binding site 2 out of 2 in the Crystal Structure of RNF168 (Ring)-UBCH5C Fused to H2A-H2B Via A 20- Residue Linker (Crystallization Condition 2)


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Crystal Structure of RNF168 (Ring)-UBCH5C Fused to H2A-H2B Via A 20- Residue Linker (Crystallization Condition 2) within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn4002 b:49.6 occ:1.00 SG A:CYS39 2.3 53.5 1.0 SG A:CYS36 2.3 61.8 1.0 SG A:CYS19 2.3 60.5 1.0 SG A:CYS16 2.3 57.0 1.0 HH22 A:ARG91 2.6 102.5 1.0 HB2 A:CYS39 2.8 63.3 1.0 H A:CYS19 3.0 70.5 1.0 CB A:CYS39 3.1 52.7 1.0 HB3 A:CYS19 3.2 72.8 1.0 HB3 A:CYS16 3.3 74.9 1.0 CB A:CYS19 3.4 60.7 1.0 CB A:CYS16 3.4 62.4 1.0 HH12 A:ARG91 3.4 100.0 1.0 HB A:ILE18 3.5 65.4 1.0 NH2 A:ARG91 3.5 85.3 1.0 H A:CYS36 3.5 62.9 1.0 HB2 A:CYS16 3.5 74.9 1.0 HB3 A:CYS36 3.7 71.2 1.0 H A:CYS39 3.7 67.0 1.0 CB A:CYS36 3.7 59.3 1.0 HB3 A:CYS39 3.7 63.3 1.0 N A:CYS19 3.8 58.7 1.0 HH21 A:ARG91 4.0 102.5 1.0 NH1 A:ARG91 4.1 83.3 1.0 N A:CYS36 4.1 52.4 1.0 HB2 A:CYS19 4.1 72.8 1.0 CA A:CYS19 4.2 62.2 1.0 CZ A:ARG91 4.3 79.5 1.0 CA A:CYS39 4.3 53.5 1.0 N A:CYS39 4.3 55.8 1.0 H A:ILE18 4.3 66.3 1.0 HB2 A:CYS36 4.4 71.2 1.0 HB2 A:LEU35 4.4 60.4 1.0 CB A:ILE18 4.4 54.5 1.0 CA A:CYS36 4.4 54.7 1.0 H A:GLU21 4.5 85.5 1.0 HA A:CYS39 4.6 64.2 1.0 H A:MET20 4.7 76.0 1.0 HG22 A:ILE18 4.7 61.5 1.0 O A:CYS36 4.8 55.0 1.0 O A:GLU21 4.8 77.0 1.0 HB2 A:PRO38 4.8 67.6 1.0 HB2 A:GLU21 4.8 95.8 1.0 CA A:CYS16 4.8 61.9 1.0 C A:ILE18 4.8 56.2 1.0 HD23 A:LEU23 4.9 73.5 1.0 HH11 A:ARG91 4.9 100.0 1.0 HA A:CYS19 4.9 74.7 1.0 C A:CYS36 4.9 54.1 1.0 C A:CYS19 5.0 62.5 1.0 HG13 A:ILE18 5.0 65.6 1.0 N A:ILE18 5.0 55.2 1.0 CA A:ILE18 5.0 57.0 1.0

Q.Hu, M.V.Botuyan, G.Mer. Crystal Structure of RNF168 (Ring)-UBCH5C Fused to H2A-H2B Via A 20-Residue Linker (Crystallization Condition 2) To Be Published.